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Effect of α-tocopherol on free thiol content and SOD activity in K562 cells

Efekat α-tokoferola na količinu slobodnih tiola i SOD aktivnost kod K562 ćelija

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2007
2007_leskovac_325.pdf (935.9Kb)
Authors
Spasić, Snežana
Martinov, Olga B.
Bogdanović, Višnja
Miletić, Srđan
Vrvić, Miroslav
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Abstract
We examined effect of α-tocopherol addition and influence of nitric oxide from sodium nitroprusside (SNP) on free thiol content and SOD activity in exponentially growing K562 cells (human erythroleukaemia) in RPMI 1640 (Sigma) supplemented with 2 mM glutamine, 10 % heat-inactivated fetal calf serum and antibiotics at 37 oC in a humidified 5 % carbon dioxide atmosphere. Cells concentration was of 0.85 x 106 per flask. Our results indicate potential dual role of α-tocopherol: induction of more reduced cell state (increased free -SH) and increased resistance of cancer cells to treatment which induce oxidative stress (increased SOD). α-Tocopherol pretreated K562 cells do not expected respond on the SNP treatment. .
Ispitivali smo efekat dodatka α-tokoferola i uticaj azot-oksida iz natrijum-nitroprusida (NNP) na sadržaj slobodnih tiol grupa i SOD aktivnost kod K562 ćelija (humana eritroleukemija) u eksponencijalnoj fazi rasta, koje su gajene u RPMI 1640 (Sigma) sa dodatkom 2 mM glutamina, 10 % toplotom-inaktiviranog fetalnog govedjeg seruma i antibiotika, na 37 oC u vlažnoj atmosferi sa 5 % ugljen-dioksida. Koncentracija ćelija bila je 0,85 x 106 po posudi. Naši rezultati ukazuju na moguću dvostruku ulogu α-tokoferola kod ovih ćelija: indukciju više redukovanog stanja (porast slobodnih -SH grupa), ali i porast njihove otpornosti na tretmane koji indukuju oksidacioni stres (porast SOD aktivnosti). α-Tokoferolom pretretirane K562 ćelije ne daju očekivani odgovor na tretman sa NNP. .
Source:
Zbornik radova Tehnološkog fakulteta, Leskovac, 2007, 16, 51-56

ISSN: 0352-6542

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http://cer.ihtm.bg.ac.rs/handle/123456789/325
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