Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study

Bolic, Bojana; Mijuskovic, Ana; Popovic-Bijelic, Ana; Nikolić-Kokić, Aleksandra; Spasić, Snežana; Blagojevic, Dusko; Spasic, Mihajlo B; Spasojević, Ivan

doi:10.1016/j.niox.2015.09.008

2015

10.1016j.niox.2015.09.008.pdf (739.7Kb)

Authors

Bolic, Bojana
Mijuskovic, Ana
Popovic-Bijelic, Ana
Nikolić-Kokić, Aleksandra

Spasić, Snežana

Blagojevic, Dusko
Spasic, Mihajlo B
Spasojević, Ivan

Article (Accepted Version)

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Abstract

Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.

Keywords:

Superoxide dismutase / EPR / H2S / Iron / Manganese / Copper

Source:
Nitric Oxide-Biology and Chemistry, 2015, 51, 19-23

Publisher:

Academic Press Inc Elsevier Science, San Diego

Projects:

Molecular mechanisms of redox signalling in homeostasis: adaptation and pathology (RS-173014)
Biomarkers in neurodegenerative and malignant processes (RS-41005)

Note:

This is peer-reviewed version of the artcle: B. Bolić, A. Mijušković, A. Popović-Bijelić, A. Nikolić-Kokić, S. Spasić, D. Blagojević, M.B. Spasić, I. Spasojević, Reactions of superoxide dismutases with HS−/H2S and superoxide radical anion: An in vitro EPR study, Nitric Oxide. 51 (2015) 19–23. https://doi.org/10.1016/j.niox.2015.09.008
Published version: http://cer.ihtm.bg.ac.rs/handle/123456789/1787

DOI: 10.1016/j.niox.2015.09.008

ISSN: 1089-8603

PubMed: 26436856

WoS: 000366065100003

Scopus: 2-s2.0-84944719548

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	5
	4

TY  - JOUR
AU  - Bolic, Bojana
AU  - Mijuskovic, Ana
AU  - Popovic-Bijelic, Ana
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Snežana
AU  - Blagojevic, Dusko
AU  - Spasic, Mihajlo B
AU  - Spasojević, Ivan
PY  - 2015
UR  - http://cer.ihtm.bg.ac.rs/handle/123456789/3187
AB  - Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Nitric Oxide-Biology and Chemistry
T1  - Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study
VL  - 51
SP  - 19
EP  - 23
DO  - 10.1016/j.niox.2015.09.008
ER  -

@article{
author = "Bolic, Bojana and Mijuskovic, Ana and Popovic-Bijelic, Ana and Nikolić-Kokić, Aleksandra and Spasić, Snežana and Blagojevic, Dusko and Spasic, Mihajlo B and Spasojević, Ivan",
year = "2015",
url = "http://cer.ihtm.bg.ac.rs/handle/123456789/3187",
abstract = "Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Nitric Oxide-Biology and Chemistry",
title = "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study",
volume = "51",
pages = "19-23",
doi = "10.1016/j.niox.2015.09.008"
}

Bolic, B., Mijuskovic, A., Popovic-Bijelic, A., Nikolić-Kokić, A., Spasić, S., Blagojevic, D., Spasic, M. B.,& Spasojević, I. (2015). Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study.
Nitric Oxide-Biology and Chemistry
Academic Press Inc Elsevier Science, San Diego., 51, 19-23.
https://doi.org/10.1016/j.niox.2015.09.008

Bolic B, Mijuskovic A, Popovic-Bijelic A, Nikolić-Kokić A, Spasić S, Blagojevic D, Spasic MB, Spasojević I. Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study. Nitric Oxide-Biology and Chemistry. 2015;51:19-23

Bolic Bojana, Mijuskovic Ana, Popovic-Bijelic Ana, Nikolić-Kokić Aleksandra, Spasić Snežana, Blagojevic Dusko, Spasic Mihajlo B, Spasojević Ivan, "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study" Nitric Oxide-Biology and Chemistry, 51 (2015):19-23,
https://doi.org/10.1016/j.niox.2015.09.008 .