Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study
Authors
Bolic, BojanaMijuskovic, Ana
Popovic-Bijelic, Ana
Nikolić-Kokić, Aleksandra

Spasić, Snežana

Blagojevic, Dusko
Spasic, Mihajlo B
Spasojević, Ivan

Article (Accepted Version)
Metadata
Show full item recordAbstract
Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.
Keywords:
Superoxide dismutase / EPR / H2S / Iron / Manganese / CopperSource:
Nitric Oxide-Biology and Chemistry, 2015, 51, 19-23Publisher:
- Academic Press Inc Elsevier Science, San Diego
Projects:
- Molecular mechanisms of redox signalling in homeostasis: adaptation and pathology (RS-173014)
- Biomarkers in neurodegenerative and malignant processes (RS-41005)
Note:
- This is peer-reviewed version of the artcle: B. Bolić, A. Mijušković, A. Popović-Bijelić, A. Nikolić-Kokić, S. Spasić, D. Blagojević, M.B. Spasić, I. Spasojević, Reactions of superoxide dismutases with HS−/H2S and superoxide radical anion: An in vitro EPR study, Nitric Oxide. 51 (2015) 19–23. https://doi.org/10.1016/j.niox.2015.09.008
- Published version: http://cer.ihtm.bg.ac.rs/handle/123456789/1787
DOI: 10.1016/j.niox.2015.09.008
ISSN: 1089-8603
PubMed: 26436856