Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study
Authors
Bolic, BojanaMijuskovic, Ana
Popovic-Bijelic, Ana
Nikolić-Kokić, Aleksandra

Spasić, Snežana

Blagojevic, Dusko
Spasić, Mihajlo

Spasojević, Ivan

Article (Accepted Version)
Metadata
Show full item recordAbstract
Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.
Keywords:
Superoxide dismutase / EPR / H2S / Iron / Manganese / CopperSource:
Nitric Oxide-Biology and Chemistry, 2015, 51, 19-23Publisher:
- Academic Press Inc Elsevier Science, San Diego
Funding / projects:
- Molecular mechanisms of redox signalling in homeostasis: adaptation and pathology (RS-173014)
- Biomarkers in neurodegenerative and malignant processes (RS-41005)
Note:
- This is peer-reviewed version of the artcle: B. Bolić, A. Mijušković, A. Popović-Bijelić, A. Nikolić-Kokić, S. Spasić, D. Blagojević, M.B. Spasić, I. Spasojević, Reactions of superoxide dismutases with HS−/H2S and superoxide radical anion: An in vitro EPR study, Nitric Oxide. 51 (2015) 19–23. https://doi.org/10.1016/j.niox.2015.09.008
- Published version: http://cer.ihtm.bg.ac.rs/handle/123456789/1787
DOI: 10.1016/j.niox.2015.09.008
ISSN: 1089-8603
PubMed: 26436856
WoS: 000366065100003
Scopus: 2-s2.0-84944719548
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Institution/Community
IHTMTY - JOUR AU - Bolic, Bojana AU - Mijuskovic, Ana AU - Popovic-Bijelic, Ana AU - Nikolić-Kokić, Aleksandra AU - Spasić, Snežana AU - Blagojevic, Dusko AU - Spasić, Mihajlo AU - Spasojević, Ivan PY - 2015 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/3187 AB - Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S. PB - Academic Press Inc Elsevier Science, San Diego T2 - Nitric Oxide-Biology and Chemistry T1 - Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study VL - 51 SP - 19 EP - 23 DO - 10.1016/j.niox.2015.09.008 ER -
@article{ author = "Bolic, Bojana and Mijuskovic, Ana and Popovic-Bijelic, Ana and Nikolić-Kokić, Aleksandra and Spasić, Snežana and Blagojevic, Dusko and Spasić, Mihajlo and Spasojević, Ivan", year = "2015", abstract = "Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.", publisher = "Academic Press Inc Elsevier Science, San Diego", journal = "Nitric Oxide-Biology and Chemistry", title = "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study", volume = "51", pages = "19-23", doi = "10.1016/j.niox.2015.09.008" }
Bolic, B., Mijuskovic, A., Popovic-Bijelic, A., Nikolić-Kokić, A., Spasić, S., Blagojevic, D., Spasić, M.,& Spasojević, I.. (2015). Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry Academic Press Inc Elsevier Science, San Diego., 51, 19-23. https://doi.org/10.1016/j.niox.2015.09.008
Bolic B, Mijuskovic A, Popovic-Bijelic A, Nikolić-Kokić A, Spasić S, Blagojevic D, Spasić M, Spasojević I. Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry. 2015;51:19-23. doi:10.1016/j.niox.2015.09.008 .
Bolic, Bojana, Mijuskovic, Ana, Popovic-Bijelic, Ana, Nikolić-Kokić, Aleksandra, Spasić, Snežana, Blagojevic, Dusko, Spasić, Mihajlo, Spasojević, Ivan, "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study" in Nitric Oxide-Biology and Chemistry, 51 (2015):19-23, https://doi.org/10.1016/j.niox.2015.09.008 . .