In order to improve the sensitivity of assays for inhibitors of the enzyme acetylcholine esterase (AChE), an effective method was developed for the conversion of the organophosphate pesticides (OPs) diazinon, malathion, chlorpyrifos, azinphos-methyl and phorate into more toxic inhibitors. This was accomplished by converting them from the thio form into their oxo form using the enzyme myeloperoxidase. The oxo forms, which are the only products of conversion, were determined by AChE bioassays, using either the free enzyme, or a flow injection analysis manifold with immobilized AChE and spectrophotometric detection. All modified OPs exhibited inhibitory power at ppb levels and within 10 min. The method is considered to represent an excellent means for improving the sensitivity of assays for determination of OPs.
TY - JOUR
AU - Lazarević-Pašti, Tamara D.
AU - Momić, Tatjana
AU - Onjia, Antonije E.
AU - Vujisić, Ljubodrag V.
AU - Vasić, Vesna
PY - 2010
UR - http://cer.ihtm.bg.ac.rs/handle/123456789/3176
AB - In order to improve the sensitivity of assays for inhibitors of the enzyme acetylcholine esterase (AChE), an effective method was developed for the conversion of the organophosphate pesticides (OPs) diazinon, malathion, chlorpyrifos, azinphos-methyl and phorate into more toxic inhibitors. This was accomplished by converting them from the thio form into their oxo form using the enzyme myeloperoxidase. The oxo forms, which are the only products of conversion, were determined by AChE bioassays, using either the free enzyme, or a flow injection analysis manifold with immobilized AChE and spectrophotometric detection. All modified OPs exhibited inhibitory power at ppb levels and within 10 min. The method is considered to represent an excellent means for improving the sensitivity of assays for determination of OPs.
PB - Springer Science and Business Media LLC
T2 - Microchimica Acta
T1 - Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods
VL - 170
IS - 3-4
SP - 289
EP - 297
DO - 10.1007/s00604-010-0324-2
ER -
@article{
author = "Lazarević-Pašti, Tamara D. and Momić, Tatjana and Onjia, Antonije E. and Vujisić, Ljubodrag V. and Vasić, Vesna",
year = "2010",
url = "http://cer.ihtm.bg.ac.rs/handle/123456789/3176",
abstract = "In order to improve the sensitivity of assays for inhibitors of the enzyme acetylcholine esterase (AChE), an effective method was developed for the conversion of the organophosphate pesticides (OPs) diazinon, malathion, chlorpyrifos, azinphos-methyl and phorate into more toxic inhibitors. This was accomplished by converting them from the thio form into their oxo form using the enzyme myeloperoxidase. The oxo forms, which are the only products of conversion, were determined by AChE bioassays, using either the free enzyme, or a flow injection analysis manifold with immobilized AChE and spectrophotometric detection. All modified OPs exhibited inhibitory power at ppb levels and within 10 min. The method is considered to represent an excellent means for improving the sensitivity of assays for determination of OPs.",
publisher = "Springer Science and Business Media LLC",
journal = "Microchimica Acta",
title = "Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods",
volume = "170",
number = "3-4",
pages = "289-297",
doi = "10.1007/s00604-010-0324-2"
}
Lazarević-Pašti, T. D., Momić, T., Onjia, A. E., Vujisić, L. V.,& Vasić, V. (2010). Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods.
Microchimica Acta
Springer Science and Business Media LLC., 170(3-4), 289-297.
https://doi.org/10.1007/s00604-010-0324-2
Lazarević-Pašti TD, Momić T, Onjia AE, Vujisić LV, Vasić V. Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods. Microchimica Acta. 2010;170(3-4):289-297
Lazarević-Pašti Tamara D., Momić Tatjana, Onjia Antonije E., Vujisić Ljubodrag V., Vasić Vesna, "Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods" Microchimica Acta, 170, no. 3-4 (2010):289-297,
https://doi.org/10.1007/s00604-010-0324-2 .
