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Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods

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2010
Authors
Lazarević-Pašti, Tamara D.
Momić, Tatjana
Onjia, Antonije E.
Vujisić, Ljubodrag V.
Vasić, Vesna
Article (Published version)
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Abstract
In order to improve the sensitivity of assays for inhibitors of the enzyme acetylcholine esterase (AChE), an effective method was developed for the conversion of the organophosphate pesticides (OPs) diazinon, malathion, chlorpyrifos, azinphos-methyl and phorate into more toxic inhibitors. This was accomplished by converting them from the thio form into their oxo form using the enzyme myeloperoxidase. The oxo forms, which are the only products of conversion, were determined by AChE bioassays, using either the free enzyme, or a flow injection analysis manifold with immobilized AChE and spectrophotometric detection. All modified OPs exhibited inhibitory power at ppb levels and within 10 min. The method is considered to represent an excellent means for improving the sensitivity of assays for determination of OPs.
Keywords:
Organophosphate pesticides / Myeloperoxidase / Oxidation / Bioanalytics / Acethylcholinesterase
Source:
Microchimica Acta, 2010, 170, 3-4, 289-297
Publisher:
  • Springer Science and Business Media LLC

DOI: 10.1007/s00604-010-0324-2

ISSN: 0026-3672; 1436-5073

WoS: 000281384000011

Scopus: 2-s2.0-77956180737
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11
URI
http://cer.ihtm.bg.ac.rs/handle/123456789/3176
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IHTM

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