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dc.creatorPešić, Milja
dc.creatorBožić, Nataša
dc.creatorLopez, Carmen
dc.creatorLončar, Nikola
dc.creatorAlvaro, Gregorio
dc.creatorVujčić, Zoran
dc.date.accessioned2019-10-23T13:54:13Z
dc.date.available2015-06-09
dc.date.issued2014
dc.identifier.issn1359-5113
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/3162
dc.description.abstractChloroperoxidase from Caldariomyces fumago (CPO, EC 1.11.1.10) is one of the most interesting enzymes from the group of heme peroxidases and has been extensively applied in synthetic processes. Nevertheless, the practical application of CPO is limited due to its very low operational stability, especially in the presence of peroxidative compounds. For this reason, effect of chemical modifications of CPO in the stability of the enzyme was studied. Side-chain selective modifications of amino groups of Lys residues, and carboxyl groups of Asp and Glu residues, as well as crosslinking and periodate oxidation of sugar moiety were carried out. The stability of modified CPOs was evaluated at elevated pH and temperature, and in the presence of tert-butyl hydroperoxide. Effect of modification of CPO on the performance of the reaction of Cbz-ethanolamine oxidation was studied as well. Those modifications that involved carboxyl groups via carbodiimide coupled method and the periodate oxidation of the sugar moiety produced better catalysts than native CPO in terms of stability and activity at elevated pH values and temperatures.en
dc.publisherElsevier Sci Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172048/RS//
dc.relationSpanish MICINN [CTQ2011-28398-CO2-01]
dc.relationJoint Serbian-Spanish Action [A IB2010 SE-00122]
dc.rightsembargoedAccess
dc.sourceProcess Biochemistry
dc.subjectChemical modificationsen
dc.subjectChloroperoxidase from Caldariomyces fumagoen
dc.subjectCbz-ethanolamine oxidationen
dc.subjectCbz-glycinal synthesisen
dc.subjectPeroxide dependent inactivationen
dc.titleChemical modification of chloroperoxidase for enhanced stability and activityen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractПесиц, Миља; Божић, Наташа; Лопез, Цармен; Aлваро, Грегорио; Вујчић, Зоран; Лонцар, Никола;
dc.citation.volume49
dc.citation.issue9
dc.citation.spage1472
dc.citation.epage1479
dc.citation.other49(9): 1472-1479
dc.citation.rankM21
dc.description.otherThis is the peer-reviewed version of the article: : Pesic M, Bozic N, Lopez C, Loncar N, Alvaro G, Vujcic Z, Chemical modification of chloroperoxidase for enhanced stability and activity, Process Biochemistry (2014), [http://dx.doi.org/10.1016/j.procbio.2014.05.025]
dc.description.other[http://cer.ihtm.bg.ac.rs/handle/123456789/1428]
dc.identifier.doi10.1016/j.procbio.2014.05.025
dc.identifier.rcubConv_3216
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs/bitstream/id/14355/j.procbio.2014.05.025.pdf
dc.identifier.scopus2-s2.0-84906787968
dc.identifier.wos000341549600013
dc.type.versionacceptedVersion


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