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dc.creatorDojnov, Biljana
dc.creatorGrujić, Marica
dc.creatorVujčić, Zoran
dc.date.accessioned2019-10-14T20:51:32Z
dc.date.available2016-04-02
dc.date.available2019-10-14T20:51:32Z
dc.date.issued2015
dc.identifier.issn0173-0835
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/3408
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/3144
dc.description.abstractA method for zymographic detection of specific cellulases in a complex (endocellulase, exocellulase, and cellobiase) from crude fermentation extracts, after a single electrophoretic separation, is described in this paper. Cellulases were printed onto a membrane and, subsequently, substrate gel. Cellobiase isoforms were detected on the membrane using esculine as substrate, endocellulase isoforms on substrate gel with copolymerized carboxymethyl cellulose (CMC), while exocellulase isoforms were detected in electrophoresis gel with 4-methylumbelliferyl--d-cellobioside (MUC). This can be a useful additional tool for monitoring and control of fungal cellulase production in industrial processes and fundamental research, screening for particular cellulase producers, or testing of new lignocellulose substrates.en
dc.publisherWiley-Blackwell, Hoboken
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172048/RS//
dc.rightsembargoedAccess
dc.sourceElectrophoresis
dc.subjectbeta -Glucosidaseen
dc.subjectCellulaseen
dc.subjectEndocellulaseen
dc.subjectExocellulaseen
dc.subjectSSFen
dc.subjectTrichodermaen
dc.subjectZymogramen
dc.titleReliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complexen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractДојнов, Биљана; Вујчић, Зоран; Грујић, Марица;
dc.citation.volume36
dc.citation.issue15
dc.citation.spage1724
dc.citation.epage1727
dc.citation.other36(15): 1724-1727
dc.citation.rankM22
dc.description.otherThis is the peer-reviewed version of the following article: Dojnov, B.; Grujić, M.; Vujčić, Z. Reliable Simultaneous Zymographic Method of Characterization of Cellulolytic Enzymes from Fungal Cellulase Complex. Electrophoresis 2015, 36 (15), 1724–1727. [https://doi.org/10.1002/elps.201400541]
dc.description.other[http://cer.ihtm.bg.ac.rs/handle/123456789/1713]
dc.identifier.pmid25873475
dc.identifier.doi10.1002/elps.201400541
dc.identifier.rcubKon_2894
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs/bitstream/id/14200/Reliable_simultaneous_zymographic_acc_2015.pdf
dc.identifier.scopus2-s2.0-84938380974
dc.identifier.wos000359057600008
dc.type.versionacceptedVersionen


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