Chemical composition, cytotoxic and antioxidative activities of ethanolic extracts of propolis on HCT-116 cell line

Zizic, Jovana B.; Vukovic, Nenad L.; Jadranin, Milka; Anđelković, Boban D.; Tešević, Vele; Kacaniova, Miroslava M.; Sukdolak, Slobodan B.; Marković, Snežana D.

doi:10.1002/jsfa.6132

2013

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Authors

Zizic, Jovana B.
Vukovic, Nenad L.

Jadranin, Milka

Anđelković, Boban D.

Tešević, Vele

Kacaniova, Miroslava M.

Sukdolak, Slobodan B.
Marković, Snežana D.

Article (Accepted Version)

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Abstract

BACKGROUND Propolis is a complex resinous sticky substance that honeybees collect from buds and exudates of various plants. Owing to its versatile biological and pharmacological activities, propolis is widely used in medicines, cosmetics and foods. The aim of this study was to evaluate the cytotoxic and antioxidative effects of various ethanolic extracts of propolis (EEPs) on human colon cancer cell line HCT-116 and compare them with their composition determined by HPLC-DAD. RESULTS The most abundant flavonoids in all samples were chrysin, pinocembrin and galangin (12.697-40.811 mu gmg(-1)), while the main phenolic acids were caffeic acid, ferulic acid and isoferulic acid. Dose- and time-dependent inhibition of growth of HCT-116 cells was observed for all propolis samples, with IC50 values ranging from 26.33 to 143.09 mu gmL(-1). Differences in cytotoxic activity of propolis samples were associated with differences in their composition. All EEP samples reduced both superoxide anion rad...

Keywords:

propolis / polyphenols / cytotoxicity / antioxidants / HCT-116 cell line

Source:
Journal of the Science of Food and Agriculture, 2013, 93, 12, 3001-3009

Publisher:

Wiley, Hoboken

Projects:

Note:

This is the peer-reviewed version of the following article: Žižić, J. B.; Vuković, N. L.; Jadranin, M.; Anđelković, B. D.; Tešević, V.; Kacaniova, M. M.; Sukdolak, S. B.; Markovic, S. D. Chemical Composition, Cytotoxic and Antioxidative Activities of Ethanolic Extracts of Propolis on HCT-116 Cell Line. Journal of the Science of Food and Agriculture 2013, 93 (12), 3001–3009. https://doi.org/10.1002/jsfa.6132
http://cer.ihtm.bg.ac.rs/handle/123456789/1213

DOI: 10.1002/jsfa.6132

ISSN: 0022-5142

PubMed: 23504630

WoS: 000323100600019

Scopus: 2-s2.0-84881025942

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TY - JOUR
AU - Zizic, Jovana B.
AU - Vukovic, Nenad L.
AU - Jadranin, Milka
AU - Anđelković, Boban D.
AU - Tešević, Vele
AU - Kacaniova, Miroslava M.
AU - Sukdolak, Slobodan B.
AU - Marković, Snežana D.
PY - 2013
UR - http://cer.ihtm.bg.ac.rs/handle/123456789/3133
AB - BACKGROUND Propolis is a complex resinous sticky substance that honeybees collect from buds and exudates of various plants. Owing to its versatile biological and pharmacological activities, propolis is widely used in medicines, cosmetics and foods. The aim of this study was to evaluate the cytotoxic and antioxidative effects of various ethanolic extracts of propolis (EEPs) on human colon cancer cell line HCT-116 and compare them with their composition determined by HPLC-DAD. RESULTS The most abundant flavonoids in all samples were chrysin, pinocembrin and galangin (12.697-40.811 mu gmg(-1)), while the main phenolic acids were caffeic acid, ferulic acid and isoferulic acid. Dose- and time-dependent inhibition of growth of HCT-116 cells was observed for all propolis samples, with IC50 values ranging from 26.33 to 143.09 mu gmL(-1). Differences in cytotoxic activity of propolis samples were associated with differences in their composition. All EEP samples reduced both superoxide anion radical and nitrite levels and also had strong DPPH-scavenging activity. CONCLUSION All tested propolis samples had pronounced cytotoxic and antioxidative activities.
PB - Wiley, Hoboken
T2 - Journal of the Science of Food and Agriculture
T1 - Chemical composition, cytotoxic and antioxidative activities of ethanolic extracts of propolis on HCT-116 cell line
VL - 93
IS - 12
SP - 3001
EP - 3009
DO - 10.1002/jsfa.6132
ER -

@article{
author = "Zizic, Jovana B. and Vukovic, Nenad L. and Jadranin, Milka and Anđelković, Boban D. and Tešević, Vele and Kacaniova, Miroslava M. and Sukdolak, Slobodan B. and Marković, Snežana D.",
year = "2013",
url = "http://cer.ihtm.bg.ac.rs/handle/123456789/3133",
abstract = "BACKGROUND Propolis is a complex resinous sticky substance that honeybees collect from buds and exudates of various plants. Owing to its versatile biological and pharmacological activities, propolis is widely used in medicines, cosmetics and foods. The aim of this study was to evaluate the cytotoxic and antioxidative effects of various ethanolic extracts of propolis (EEPs) on human colon cancer cell line HCT-116 and compare them with their composition determined by HPLC-DAD. RESULTS The most abundant flavonoids in all samples were chrysin, pinocembrin and galangin (12.697-40.811 mu gmg(-1)), while the main phenolic acids were caffeic acid, ferulic acid and isoferulic acid. Dose- and time-dependent inhibition of growth of HCT-116 cells was observed for all propolis samples, with IC50 values ranging from 26.33 to 143.09 mu gmL(-1). Differences in cytotoxic activity of propolis samples were associated with differences in their composition. All EEP samples reduced both superoxide anion radical and nitrite levels and also had strong DPPH-scavenging activity. CONCLUSION All tested propolis samples had pronounced cytotoxic and antioxidative activities.",
publisher = "Wiley, Hoboken",
journal = "Journal of the Science of Food and Agriculture",
title = "Chemical composition, cytotoxic and antioxidative activities of ethanolic extracts of propolis on HCT-116 cell line",
volume = "93",
number = "12",
pages = "3001-3009",
doi = "10.1002/jsfa.6132"
}

Zizic, J. B., Vukovic, N. L., Jadranin, M., Anđelković, B. D., Tešević, V., Kacaniova, M. M., Sukdolak, S. B.,& Marković, S. D. (2013). Chemical composition, cytotoxic and antioxidative activities of ethanolic extracts of propolis on HCT-116 cell line.
Journal of the Science of Food and Agriculture
Wiley, Hoboken., 93(12), 3001-3009.
https://doi.org/10.1002/jsfa.6132

Zizic JB, Vukovic NL, Jadranin M, Anđelković BD, Tešević V, Kacaniova MM, Sukdolak SB, Marković SD. Chemical composition, cytotoxic and antioxidative activities of ethanolic extracts of propolis on HCT-116 cell line. Journal of the Science of Food and Agriculture. 2013;93(12):3001-3009

Zizic Jovana B., Vukovic Nenad L., Jadranin Milka, Anđelković Boban D., Tešević Vele, Kacaniova Miroslava M., Sukdolak Slobodan B., Marković Snežana D., "Chemical composition, cytotoxic and antioxidative activities of ethanolic extracts of propolis on HCT-116 cell line" Journal of the Science of Food and Agriculture, 93, no. 12 (2013):3001-3009,
https://doi.org/10.1002/jsfa.6132 .