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dc.creatorAvramov Ivić, Milka
dc.creatorLović, Jelena
dc.creatorStevanović, Sanja
dc.creatorNikolić, Nebojša
dc.creatorTrišović, Nemanja
dc.creatorLađarević, Jelena
dc.creatorVuković, Dragan
dc.creatorDrmanić, Saša Ž.
dc.creatorMladenović, Aleksandar R.
dc.creatorJadranin, Milka
dc.creatorPetrović, Slobodan D.
dc.creatorMijin, Dušan
dc.date.accessioned2019-09-05T11:12:43Z
dc.date.available2019-09-05T11:12:43Z
dc.date.issued2019
dc.identifier.issn1572-6657
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/3062
dc.description.abstractEsomeprazole is the most effective of the proton-pump inhibitors for the acid-related diseases and at first was examined for the electroanalytical purposes. The drug standard and as a content of injection powder was investigated by cyclic voltammetry (CV) and quantitatively determined using square wave voltammetry (SWV) via its electrooxidation at Au electrode in 0.05 M NaHCO3. SWV showed a linear dependency of the anodic peak currents vs. esomeprazole standard concentrations in the range from 3.0 to 500 μg mL−1 with the values of limit of detection (LOD) and limit of quantification (LOQ): 1.4 and 4.6 μg mL−1, respectively. Using the constructed and validated calibration curve, the values of unknown esomeprazole concentrations in injection powder and in human serum spiked with standard were determined. Before the electrochemical oxidation, it was shown by atomic force microscopy (AFM) that the small esomeprazole islands formed inside holes were visible and their diameter was about 200 nm attributed to physico-chemical characteristics of esomeprazole. After the electrochemical oxidation, the morphology of esomeprazole standard on Au surface was completely changed and composed of spherical particles in a diameter between 200 and 600 nm. With esomeprazole suspended in human serum, the process of crystallization partly occurred in the form of spherical grains with the average size of these grains was about 4 μm. The analysis at the macro level done by the optical microscopy (OM) confirmed this opinion. The study of esomeprazole degradation showed that at Au electrode, after 3 h of cycling, a neglectable amount of the esomeprazole was changed. Using IrOx electrode under directed stress conditions, its almost complete degradation was realized after 3 h confirmed by high performance liquid chromatography (HPLC). Total organic carbon (TOC) analysis showed that 95% of esomeprazole was mineralized. The HPLC and Liquid chromatography-mass spectrometry (LC-MS) study revealed the formation of 4-hydroxy omeprazole sulphide, 4-hydroxy omeprazole sulphone, esomeprazole sulphone and methylated esomeprazole.sr
dc.language.isoensr
dc.publisherElseviersr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172013/RS//sr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172060/RS//sr
dc.rightsrestrictedAccesssr
dc.sourceJournal of Electroanalytical Chemistrysr
dc.subjectEsomeprazolesr
dc.subjectSWV determination at Ausr
dc.subjectAu surface analysis by AFMsr
dc.subjectOM Degradation at IrOxsr
dc.subjectHPLCsr
dc.subjectLC-MSsr
dc.titleElectrochemical behavior of esomeprazole: Its determination at Au electrode as standard and in injection powder combined with the study of its degradationsr
dc.typearticlesr
dc.rights.licenseARRsr
dcterms.abstractЛовић, Јелена Д.; Aврамов Ивић, Милка; Јадранин, Милка Б.; Петровић, Слободан Д.; Николић, Небојша Д.; Стевановић, Сања И.; Мијин, Душан Ж.; Вуковић, Драган; Дрманић, Саша Ж.; Младеновић, Aлександар Р.; Лађаревић, Јелена М.; Тришовић, Немања П.;
dc.rights.holderElseviersr
dc.citation.volume848
dc.citation.spage113303
dc.citation.rankM21~
dc.identifier.doi10.1016/j.jelechem.2019.113303
dc.identifier.scopus2-s2.0-85069593343
dc.identifier.wos000504505400031
dc.type.versionpublishedVersionsr


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