The influence of four low-frequency magnetic field (MF) ranges 10–300 Hz, 10–100 Hz, 10–50 Hz and 50–100 Hz in scanning regime (all frequencies from selected range were scanned during 100 s repetitively during 24 h) on baker's yeast cells Saccharomyces cerevisiae was examined by continuous measurements of cumulative O2 consumption and cumulative CO2 production over 24 h with Micro-Oxymax® respirometer. Besides respiration activity, measurements of cell growth and glucose uptake were performed as well. Statistical analysis indicated that, among all investigated low-frequency MF ranges, range from 10 Hz to 50 Hz had the greatest influence to yeast cell respiration and cell growth. More precisely, for this region, paired two sample one-tail t-test showed statistically significant differences in cumulative O2 consumption, cumulative CO2 production and S. cerevisiae cell number. Moreover samples exposed to MF range from 10 Hz to 50 Hz showed the same behavior in all five replicates: lower c...umulative O2 consumption, higher cumulative CO2 production and higher cell number compared to control sample. This could be important from the application aspect, in industry (food, feed, brewery etc.) and biotechnology, because changes in cells metabolism are not caused by chemical treatment.
Keywords:
Low-frequency magnetic field / Saccharomyces cerevisiae / Cell respiration / Oxygen consumption / Carbon dioxide production / Non-chemical cells treatment
Source:
Chemical Engineering & Processing: Process Intensification, 2019, 143, 107593-
TY - JOUR
AU - Bubanja, Itana Nuša
AU - Lončarević, Branka
AU - Lješević, Marija
AU - Beškoski, Vladimir
AU - Gojgić-Cvijović, Gordana
AU - Velikić, Zoran
AU - Stanisavljev, Dragomir
PY - 2019
UR - http://cer.ihtm.bg.ac.rs/handle/123456789/3059
AB - The influence of four low-frequency magnetic field (MF) ranges 10–300 Hz, 10–100 Hz, 10–50 Hz and 50–100 Hz in scanning regime (all frequencies from selected range were scanned during 100 s repetitively during 24 h) on baker's yeast cells Saccharomyces cerevisiae was examined by continuous measurements of cumulative O2 consumption and cumulative CO2 production over 24 h with Micro-Oxymax® respirometer. Besides respiration activity, measurements of cell growth and glucose uptake were performed as well. Statistical analysis indicated that, among all investigated low-frequency MF ranges, range from 10 Hz to 50 Hz had the greatest influence to yeast cell respiration and cell growth. More precisely, for this region, paired two sample one-tail t-test showed statistically significant differences in cumulative O2 consumption, cumulative CO2 production and S. cerevisiae cell number. Moreover samples exposed to MF range from 10 Hz to 50 Hz showed the same behavior in all five replicates: lower cumulative O2 consumption, higher cumulative CO2 production and higher cell number compared to control sample. This could be important from the application aspect, in industry (food, feed, brewery etc.) and biotechnology, because changes in cells metabolism are not caused by chemical treatment.
PB - Elsevier
T2 - Chemical Engineering & Processing: Process Intensification
T1 - The influence of low-frequency magnetic field regions on the Saccharomyces cerevisiae respiration and growth
VL - 143
SP - 107593
DO - 10.1016/j.cep.2019.107593
ER -
@article{
author = "Bubanja, Itana Nuša and Lončarević, Branka and Lješević, Marija and Beškoski, Vladimir and Gojgić-Cvijović, Gordana and Velikić, Zoran and Stanisavljev, Dragomir",
year = "2019",
url = "http://cer.ihtm.bg.ac.rs/handle/123456789/3059",
abstract = "The influence of four low-frequency magnetic field (MF) ranges 10–300 Hz, 10–100 Hz, 10–50 Hz and 50–100 Hz in scanning regime (all frequencies from selected range were scanned during 100 s repetitively during 24 h) on baker's yeast cells Saccharomyces cerevisiae was examined by continuous measurements of cumulative O2 consumption and cumulative CO2 production over 24 h with Micro-Oxymax® respirometer. Besides respiration activity, measurements of cell growth and glucose uptake were performed as well. Statistical analysis indicated that, among all investigated low-frequency MF ranges, range from 10 Hz to 50 Hz had the greatest influence to yeast cell respiration and cell growth. More precisely, for this region, paired two sample one-tail t-test showed statistically significant differences in cumulative O2 consumption, cumulative CO2 production and S. cerevisiae cell number. Moreover samples exposed to MF range from 10 Hz to 50 Hz showed the same behavior in all five replicates: lower cumulative O2 consumption, higher cumulative CO2 production and higher cell number compared to control sample. This could be important from the application aspect, in industry (food, feed, brewery etc.) and biotechnology, because changes in cells metabolism are not caused by chemical treatment.",
publisher = "Elsevier",
journal = "Chemical Engineering & Processing: Process Intensification",
title = "The influence of low-frequency magnetic field regions on the Saccharomyces cerevisiae respiration and growth",
volume = "143",
pages = "107593",
doi = "10.1016/j.cep.2019.107593"
}
Bubanja, I. N., Lončarević, B., Lješević, M., Beškoski, V., Gojgić-Cvijović, G., Velikić, Z.,& Stanisavljev, D. (2019). The influence of low-frequency magnetic field regions on the Saccharomyces cerevisiae respiration and growth.
Chemical Engineering & Processing: Process Intensification
Elsevier., 143, 107593.
https://doi.org/10.1016/j.cep.2019.107593
Bubanja IN, Lončarević B, Lješević M, Beškoski V, Gojgić-Cvijović G, Velikić Z, Stanisavljev D. The influence of low-frequency magnetic field regions on the Saccharomyces cerevisiae respiration and growth. Chemical Engineering & Processing: Process Intensification. 2019;143:107593
Bubanja Itana Nuša, Lončarević Branka, Lješević Marija, Beškoski Vladimir, Gojgić-Cvijović Gordana, Velikić Zoran, Stanisavljev Dragomir, "The influence of low-frequency magnetic field regions on the Saccharomyces cerevisiae respiration and growth" Chemical Engineering & Processing: Process Intensification, 143 (2019):107593,
https://doi.org/10.1016/j.cep.2019.107593 .
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