Integration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatus
Samo za registrovane korisnike
2020
Autori
Cvetković, Mirjana
Damjanović, Ana
Stanojković, Tatjana

Đorđević, Iris

Tešević, Vele

Milosavljević, Slobodan

Gođevac, Dejan

Članak u časopisu (Objavljena verzija)

Elsevier
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Metabolomics generate a profile of small molecules from plant extracts, which could be directly responsible for bioactivity effects. Using dry-column flash chromatography enabled a rapid and inexpensive method for the very efficient separation of plant extract with a high resolution. This separation method coupled to NMR and FTIR based metabolomics is applied to identify bioactive natural products. OPLS multivariate analysis method, was used for correlation the chemical composition of the plant extracts, Amphoricarpos autariatus, with the results of cytotoxic activity against Human cervical adenocarcinoma cell line (HeLa) and epithelial lung cancer cell line
(A549). In this way, the highest contribution to the cytotoxic activity was recorded for the guaianolide sesquiterpene lactones named amphoricarpolides. The compounds indicated as bioactive after metabolomics analysis were tested, and their cytotoxic activity were confirmed.
Ključne reči:
Amphoricarpos autariatus / NMR / FTIR / Metabolomics / Cytotoxic activity / Dry-column flash chromatographyIzvor:
Talanta, 2020, 206, 120248-Izdavač:
- Elsevier
Finansiranje / projekti:
- Bioaktivni prirodni proizvodi samoniklih, gajenih i jestivih biljaka: određivanje struktura i aktivnosti (RS-172053)
DOI: 10.1016/j.talanta.2019.120248
ISSN: 0039-9140
WoS: 000488416200070
Scopus: 2-s2.0-85070705746
Institucija/grupa
IHTMTY - JOUR AU - Cvetković, Mirjana AU - Damjanović, Ana AU - Stanojković, Tatjana AU - Đorđević, Iris AU - Tešević, Vele AU - Milosavljević, Slobodan AU - Gođevac, Dejan PY - 2020 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/3054 AB - Metabolomics generate a profile of small molecules from plant extracts, which could be directly responsible for bioactivity effects. Using dry-column flash chromatography enabled a rapid and inexpensive method for the very efficient separation of plant extract with a high resolution. This separation method coupled to NMR and FTIR based metabolomics is applied to identify bioactive natural products. OPLS multivariate analysis method, was used for correlation the chemical composition of the plant extracts, Amphoricarpos autariatus, with the results of cytotoxic activity against Human cervical adenocarcinoma cell line (HeLa) and epithelial lung cancer cell line (A549). In this way, the highest contribution to the cytotoxic activity was recorded for the guaianolide sesquiterpene lactones named amphoricarpolides. The compounds indicated as bioactive after metabolomics analysis were tested, and their cytotoxic activity were confirmed. PB - Elsevier T2 - Talanta T1 - Integration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatus VL - 206 SP - 120248 DO - 10.1016/j.talanta.2019.120248 ER -
@article{ author = "Cvetković, Mirjana and Damjanović, Ana and Stanojković, Tatjana and Đorđević, Iris and Tešević, Vele and Milosavljević, Slobodan and Gođevac, Dejan", year = "2020", abstract = "Metabolomics generate a profile of small molecules from plant extracts, which could be directly responsible for bioactivity effects. Using dry-column flash chromatography enabled a rapid and inexpensive method for the very efficient separation of plant extract with a high resolution. This separation method coupled to NMR and FTIR based metabolomics is applied to identify bioactive natural products. OPLS multivariate analysis method, was used for correlation the chemical composition of the plant extracts, Amphoricarpos autariatus, with the results of cytotoxic activity against Human cervical adenocarcinoma cell line (HeLa) and epithelial lung cancer cell line (A549). In this way, the highest contribution to the cytotoxic activity was recorded for the guaianolide sesquiterpene lactones named amphoricarpolides. The compounds indicated as bioactive after metabolomics analysis were tested, and their cytotoxic activity were confirmed.", publisher = "Elsevier", journal = "Talanta", title = "Integration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatus", volume = "206", pages = "120248", doi = "10.1016/j.talanta.2019.120248" }
Cvetković, M., Damjanović, A., Stanojković, T., Đorđević, I., Tešević, V., Milosavljević, S.,& Gođevac, D.. (2020). Integration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatus. in Talanta Elsevier., 206, 120248. https://doi.org/10.1016/j.talanta.2019.120248
Cvetković M, Damjanović A, Stanojković T, Đorđević I, Tešević V, Milosavljević S, Gođevac D. Integration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatus. in Talanta. 2020;206:120248. doi:10.1016/j.talanta.2019.120248 .
Cvetković, Mirjana, Damjanović, Ana, Stanojković, Tatjana, Đorđević, Iris, Tešević, Vele, Milosavljević, Slobodan, Gođevac, Dejan, "Integration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatus" in Talanta, 206 (2020):120248, https://doi.org/10.1016/j.talanta.2019.120248 . .