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Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes

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2017
10.1016j.jhazmat.2016.09.037.pdf (4.537Mb)
Authors
Janović, Barbara
Collins, Andrew R.
Vujčić, Zoran
Vujčić, Miroslava
Article (Accepted Version)
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Abstract
The aim of this study was to investigate the impact of dyes on DNA before and after enzymatic decolorization by acidic horseradish peroxidase (HRP-A). The comet assay is easy and feasible method widely used to measure DNA damage and repair. The medium-throughput comet assay was employed for assessment of genotoxic effects of 8 dyes in BEAS-2B cells. We have incorporated a digestion with bacterial endonuclease (formamidopyrimidine DNA glycosylase, FPG) to detect oxidized bases in the case of single and double azo dyes, Orange II (OR2) and Amido Black 10B (AB), respectively. This allowed detection 8-oxo7,8-dihydroguanine, one of most abundant oxidized bases in nuclear DNA. In the case of AB there was no indication of DNA damage, either strand brakes or FPG-sensitive sites before and after decolorization. The OR2 induced DNA damage (in terms of percentage of DNA in comet tails). Also, the frequency of FPG-sensitive sites increased with OR2 concentration. After decolorization no DNA damagi...ng effects was seen at all. The interaction studies of OR2 and AB, before and after decolorization, with calf thymus DNA has been investigated by absorption and fluorescence spectroscopy. The results provide support for the idea that in some cases enzymatic decolorization contributes to lower genotoxicity potential.

Keywords:
DNA damage / Comet assay / Horseradish peroxidase a / Decolorization / Dye degradation / DNA interactions
Source:
Journal of Hazardous Materials, 2017, 321, 576-585
Publisher:
  • Elsevier Science Bv, Amsterdam
Projects:
  • Production, purification and characterization of enzymes and small molecules and their application as soluble or immobilized in food biotechnology, biofuels production and environmental protection (RS-172048)
  • FEBS Collaborative Experimental Scholarship, Oslo, Norway
Note:
  • This is peer-reviewed version of the artcle: B.S. Janović, A.R. Collins, Z.M. Vujčić, M.T. Vujčić, Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes, J. Hazard. Mater. 321 (2017) 576–585. https://doi.org/10.1016/j.jhazmat.2016.09.037
  • The published version: http://cer.ihtm.bg.ac.rs/handle/123456789/2070

DOI: 10.1016/j.jhazmat.2016.09.037

ISSN: 0304-3894

PubMed: 27694021

WoS: 000388777300061

Scopus: 2-s2.0-84988963283
[ Google Scholar ]
4
3
URI
http://cer.ihtm.bg.ac.rs/handle/123456789/3045
Collections
  • Radovi istraživača / Researchers' publications
Institution
IHTM
TY  - JOUR
AU  - Janović, Barbara
AU  - Collins, Andrew R.
AU  - Vujčić, Zoran
AU  - Vujčić, Miroslava
PY  - 2017
UR  - http://cer.ihtm.bg.ac.rs/handle/123456789/3045
AB  - The aim of this study was to investigate the impact of dyes on DNA before and after enzymatic decolorization by acidic horseradish peroxidase (HRP-A). The comet assay is easy and feasible method widely used to measure DNA damage and repair. The medium-throughput comet assay was employed for assessment of genotoxic effects of 8 dyes in BEAS-2B cells. We have incorporated a digestion with bacterial endonuclease (formamidopyrimidine DNA glycosylase, FPG) to detect oxidized bases in the case of single and double azo dyes, Orange II (OR2) and Amido Black 10B (AB), respectively. This allowed detection 8-oxo7,8-dihydroguanine, one of most abundant oxidized bases in nuclear DNA. In the case of AB there was no indication of DNA damage, either strand brakes or FPG-sensitive sites before and after decolorization. The OR2 induced DNA damage (in terms of percentage of DNA in comet tails). Also, the frequency of FPG-sensitive sites increased with OR2 concentration. After decolorization no DNA damaging effects was seen at all. The interaction studies of OR2 and AB, before and after decolorization, with calf thymus DNA has been investigated by absorption and fluorescence spectroscopy. The results provide support for the idea that in some cases enzymatic decolorization contributes to lower genotoxicity potential.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Hazardous Materials
T1  - Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes
VL  - 321
SP  - 576
EP  - 585
DO  - 10.1016/j.jhazmat.2016.09.037
ER  - 
@article{
author = "Janović, Barbara and Collins, Andrew R. and Vujčić, Zoran and Vujčić, Miroslava",
year = "2017",
url = "http://cer.ihtm.bg.ac.rs/handle/123456789/3045",
abstract = "The aim of this study was to investigate the impact of dyes on DNA before and after enzymatic decolorization by acidic horseradish peroxidase (HRP-A). The comet assay is easy and feasible method widely used to measure DNA damage and repair. The medium-throughput comet assay was employed for assessment of genotoxic effects of 8 dyes in BEAS-2B cells. We have incorporated a digestion with bacterial endonuclease (formamidopyrimidine DNA glycosylase, FPG) to detect oxidized bases in the case of single and double azo dyes, Orange II (OR2) and Amido Black 10B (AB), respectively. This allowed detection 8-oxo7,8-dihydroguanine, one of most abundant oxidized bases in nuclear DNA. In the case of AB there was no indication of DNA damage, either strand brakes or FPG-sensitive sites before and after decolorization. The OR2 induced DNA damage (in terms of percentage of DNA in comet tails). Also, the frequency of FPG-sensitive sites increased with OR2 concentration. After decolorization no DNA damaging effects was seen at all. The interaction studies of OR2 and AB, before and after decolorization, with calf thymus DNA has been investigated by absorption and fluorescence spectroscopy. The results provide support for the idea that in some cases enzymatic decolorization contributes to lower genotoxicity potential.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Hazardous Materials",
title = "Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes",
volume = "321",
pages = "576-585",
doi = "10.1016/j.jhazmat.2016.09.037"
}
Janović B, Collins AR, Vujčić Z, Vujčić M. Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes. Journal of Hazardous Materials. 2017;321:576-585
Janović, B., Collins, A. R., Vujčić, Z.,& Vujčić, M. (2017). Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes.
Journal of Hazardous MaterialsElsevier Science Bv, Amsterdam., 321, 576-585.
https://doi.org/10.1016/j.jhazmat.2016.09.037
Janović Barbara, Collins Andrew R., Vujčić Zoran, Vujčić Miroslava, "Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes" 321 (2017):576-585,
https://doi.org/10.1016/j.jhazmat.2016.09.037 .

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