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dc.creatorSpasojević, Dragica
dc.creatorProkopijević, Miloš
dc.creatorProdanović, Olivera
dc.creatorZelenović, Nevena
dc.creatorPolović, Natalija
dc.creatorRadotić, Ksenija
dc.creatorProdanović, Radivoje
dc.date.accessioned2019-07-10T10:51:20Z
dc.date.available2019-07-10T10:51:20Z
dc.date.issued2019
dc.identifier.issn1598-5032
dc.identifier.issn2092-7673
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/3028
dc.description.abstractDerivatives of xylans were synthesized from corncob xylan by carboxymethylation, oxidization with different molar ratios of periodate (5, 10 15 and 20 mol%) and by reductive amination with tyramine. Modifications of tyramine carboxymethyl xylans (Tyr-CMX) were confirmed by FTIR, UV and NMR spectra. Concentration of ionizable groups increased from 1.5 mmol/g for carboxymethyl xylan (CMX) to 5.4 mmol/g for Tyr-CMX oxidized with 20 mol% of periodate. All Tyr-CMXs were able to form hydrogels the cross-linking reaction with horseradish peroxidase and peroxide. Tyr-CMXs were tested for amyloglucosidase (AG) encapsulation within hydrogel microbeads obtained in a reaction of emulsion polymerization with peroxidase. Average diameter of Tyr-CMX hydrogel microbeads was 52±25 µm and after encapsulation optimization with respect to the extent of CMX modification with tyramine, the concentration of Tyr-CMX, and the amount of added AG, microbeads with AG specific activity of 2 U/mL and 20% yield of immobilization were obtained. The optimum pH of the immobilized AG was not changed compared to the soluble one, while half-life at 60 °C was increased around 10 times. The Michaelis-Menten constant for the immobilized enzyme, 1.03 mM, was significantly lower than that for the soluble one, 1.54 mM. After 5 cycles of repetitive use in batch reactor, the immobilized AG retained 68% of initial activity.sr
dc.language.isoensr
dc.publisherSpringersr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173017/RS//sr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//sr
dc.rightsrestrictedAccesssr
dc.sourceMacromolecular Research
dc.subjectxylansr
dc.subjecthydrogelsr
dc.subjecttyraminesr
dc.subjectpolymerizationsr
dc.subjectemulsionsr
dc.subjectperoxidesr
dc.titlePeroxidase-Sensitive Tyramine Carboxymethyl Xylan Hydrogels for Enzyme Encapsulationsr
dc.typearticlesr
dc.rights.licenseARRsr
dcterms.abstractПродановић, Радивоје; Половић, Наталија; Зеленовић, Невена; Продановић, Оливера; Прокопијевић, Милош; Спасојевић, Драгица; Радотић, Ксенија;
dc.rights.holderSpringersr
dc.citation.volume27
dc.citation.issue8
dc.citation.spage764
dc.citation.epage771
dc.citation.rankM22~
dc.identifier.doi10.1007/s13233-019-7111-7
dc.identifier.scopus2-s2.0-85065978831
dc.identifier.wos000482406900005
dc.type.versionpublishedVersionsr


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