High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals
Authorized Users Only
2019
Authors
Izrael‑Živković, LidijaBeškoski, Vladimir

Rikalović, Milena

Kazazić, Snježana
Shapiro, Nicole
Woyke, Tanja
Gojgić-Cvijović, Gordana

Vrvić, Miroslav

Maksimović, Nela
Karadžić, Ivanka

Article (Published version)

Springer
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Show full item recordAbstract
The strain Pseudomonas aeruginosa san ai, isolated from an extreme environment (industrial mineral cutting oil, pH 10), is able to survive and persist in the presence of a variety of pollutants such as heavy metals and organic chemicals. The genome of P. aeruginosa san ai is 6.98 Mbp long with a GC content of 66.08% and 6485 protein encoding genes. A large number of genes associated with proteins, responsible for microbial resistance to heavy metal ions andinvolved in catabolism of toxic aromatic organic compounds were identified. P.aeruginosa san ai is a highly cadmium-resistant strain. Proteome analysis of biomass after cadmium exposal confirmed a high tolerance to sublethal concentrations of cadmium (100mg/L), based on: extracellular biosorption, bioaccumulation, biofilm formation, controlled siderophore production and a pronounced metalloprotein synthesis. Proteins responsible for survival in osmostress conditions during exposure to elevated concentrations of cadmium (200mg/L) demo...nstrate a strong genetic potential of P. aeruginosa san ai for survival and adaptation. Sequencing of P. aeruginosa san ai genome provides valuable insights into the evolution and adaptation of this microbe to environmental extremes at the whole-genome level, as well as how to optimally use the strain in bioremediation of chemically polluted sites.
Keywords:
Pseudomonas aeruginosa / Genome / Environmental isolate / Chemical pollution / Cleaning polluted areasSource:
Extremophiles, 2019, 23, 4, 399-405Publisher:
- Springer
Funding / projects:
- JGI, CSP741
- Office of Science of the US Department of Energy, DE-AC02-05CH11231
- Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-43004)
DOI: 10.1007/s00792-019-01092-w
ISSN: 1431-0651; 1433-4909
PubMed: 30949775
WoS: 000471018700004
Scopus: 2-s2.0-85064264636
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Institution/Community
IHTMTY - JOUR AU - Izrael‑Živković, Lidija AU - Beškoski, Vladimir AU - Rikalović, Milena AU - Kazazić, Snježana AU - Shapiro, Nicole AU - Woyke, Tanja AU - Gojgić-Cvijović, Gordana AU - Vrvić, Miroslav AU - Maksimović, Nela AU - Karadžić, Ivanka PY - 2019 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/3026 AB - The strain Pseudomonas aeruginosa san ai, isolated from an extreme environment (industrial mineral cutting oil, pH 10), is able to survive and persist in the presence of a variety of pollutants such as heavy metals and organic chemicals. The genome of P. aeruginosa san ai is 6.98 Mbp long with a GC content of 66.08% and 6485 protein encoding genes. A large number of genes associated with proteins, responsible for microbial resistance to heavy metal ions andinvolved in catabolism of toxic aromatic organic compounds were identified. P.aeruginosa san ai is a highly cadmium-resistant strain. Proteome analysis of biomass after cadmium exposal confirmed a high tolerance to sublethal concentrations of cadmium (100mg/L), based on: extracellular biosorption, bioaccumulation, biofilm formation, controlled siderophore production and a pronounced metalloprotein synthesis. Proteins responsible for survival in osmostress conditions during exposure to elevated concentrations of cadmium (200mg/L) demonstrate a strong genetic potential of P. aeruginosa san ai for survival and adaptation. Sequencing of P. aeruginosa san ai genome provides valuable insights into the evolution and adaptation of this microbe to environmental extremes at the whole-genome level, as well as how to optimally use the strain in bioremediation of chemically polluted sites. PB - Springer T2 - Extremophiles T1 - High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals VL - 23 IS - 4 SP - 399 EP - 405 DO - 10.1007/s00792-019-01092-w ER -
@article{ author = "Izrael‑Živković, Lidija and Beškoski, Vladimir and Rikalović, Milena and Kazazić, Snježana and Shapiro, Nicole and Woyke, Tanja and Gojgić-Cvijović, Gordana and Vrvić, Miroslav and Maksimović, Nela and Karadžić, Ivanka", year = "2019", abstract = "The strain Pseudomonas aeruginosa san ai, isolated from an extreme environment (industrial mineral cutting oil, pH 10), is able to survive and persist in the presence of a variety of pollutants such as heavy metals and organic chemicals. The genome of P. aeruginosa san ai is 6.98 Mbp long with a GC content of 66.08% and 6485 protein encoding genes. A large number of genes associated with proteins, responsible for microbial resistance to heavy metal ions andinvolved in catabolism of toxic aromatic organic compounds were identified. P.aeruginosa san ai is a highly cadmium-resistant strain. Proteome analysis of biomass after cadmium exposal confirmed a high tolerance to sublethal concentrations of cadmium (100mg/L), based on: extracellular biosorption, bioaccumulation, biofilm formation, controlled siderophore production and a pronounced metalloprotein synthesis. Proteins responsible for survival in osmostress conditions during exposure to elevated concentrations of cadmium (200mg/L) demonstrate a strong genetic potential of P. aeruginosa san ai for survival and adaptation. Sequencing of P. aeruginosa san ai genome provides valuable insights into the evolution and adaptation of this microbe to environmental extremes at the whole-genome level, as well as how to optimally use the strain in bioremediation of chemically polluted sites.", publisher = "Springer", journal = "Extremophiles", title = "High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals", volume = "23", number = "4", pages = "399-405", doi = "10.1007/s00792-019-01092-w" }
Izrael‑Živković, L., Beškoski, V., Rikalović, M., Kazazić, S., Shapiro, N., Woyke, T., Gojgić-Cvijović, G., Vrvić, M., Maksimović, N.,& Karadžić, I.. (2019). High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals. in Extremophiles Springer., 23(4), 399-405. https://doi.org/10.1007/s00792-019-01092-w
Izrael‑Živković L, Beškoski V, Rikalović M, Kazazić S, Shapiro N, Woyke T, Gojgić-Cvijović G, Vrvić M, Maksimović N, Karadžić I. High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals. in Extremophiles. 2019;23(4):399-405. doi:10.1007/s00792-019-01092-w .
Izrael‑Živković, Lidija, Beškoski, Vladimir, Rikalović, Milena, Kazazić, Snježana, Shapiro, Nicole, Woyke, Tanja, Gojgić-Cvijović, Gordana, Vrvić, Miroslav, Maksimović, Nela, Karadžić, Ivanka, "High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals" in Extremophiles, 23, no. 4 (2019):399-405, https://doi.org/10.1007/s00792-019-01092-w . .