The strain Pseudomonas aeruginosa san ai, isolated from an extreme environment (industrial mineral cutting oil, pH 10), is able to survive and persist in the presence of a variety of pollutants such as heavy metals and organic chemicals. The genome of P. aeruginosa san ai is 6.98 Mbp long with a GC content of 66.08% and 6485 protein encoding genes. A large number of genes associated with proteins, responsible for microbial resistance to heavy metal ions andinvolved in catabolism of toxic aromatic organic compounds were identified. P.aeruginosa san ai is a highly cadmium-resistant strain. Proteome analysis of biomass after cadmium exposal confirmed a high tolerance to sublethal concentrations of cadmium (100mg/L), based on: extracellular biosorption, bioaccumulation, biofilm formation, controlled siderophore production and a pronounced metalloprotein synthesis. Proteins responsible for survival in osmostress conditions during exposure to elevated concentrations of cadmium (200mg/L) demo...nstrate a strong genetic potential of P. aeruginosa san ai for survival and adaptation. Sequencing of P. aeruginosa san ai genome provides valuable insights into the evolution and adaptation of this microbe to environmental extremes at the whole-genome level, as well as how to optimally use the strain in bioremediation of chemically polluted sites.
Keywords:
Pseudomonas aeruginosa / Genome / Environmental isolate / Chemical pollution / Cleaning polluted areas
Source:
Extremophiles, 2019, 23, 4, 399-405
Publisher:
Springer
Funding / projects:
JGI, CSP741
Office of Science of the US Department of Energy, DE-AC02-05CH11231
TY - JOUR
AU - Izrael‑Živković, Lidija
AU - Beškoski, Vladimir
AU - Rikalović, Milena
AU - Kazazić, Snježana
AU - Shapiro, Nicole
AU - Woyke, Tanja
AU - Gojgić-Cvijović, Gordana
AU - Vrvić, Miroslav
AU - Maksimović, Nela
AU - Karadžić, Ivanka
PY - 2019
UR - https://cer.ihtm.bg.ac.rs/handle/123456789/3026
AB - The strain Pseudomonas aeruginosa san ai, isolated from an extreme environment (industrial mineral cutting oil, pH 10), is able to survive and persist in the presence of a variety of pollutants such as heavy metals and organic chemicals. The genome of P. aeruginosa san ai is 6.98 Mbp long with a GC content of 66.08% and 6485 protein encoding genes. A large number of genes associated with proteins, responsible for microbial resistance to heavy metal ions andinvolved in catabolism of toxic aromatic organic compounds were identified. P.aeruginosa san ai is a highly cadmium-resistant strain. Proteome analysis of biomass after cadmium exposal confirmed a high tolerance to sublethal concentrations of cadmium (100mg/L), based on: extracellular biosorption, bioaccumulation, biofilm formation, controlled siderophore production and a pronounced metalloprotein synthesis. Proteins responsible for survival in osmostress conditions during exposure to elevated concentrations of cadmium (200mg/L) demonstrate a strong genetic potential of P. aeruginosa san ai for survival and adaptation. Sequencing of P. aeruginosa san ai genome provides valuable insights into the evolution and adaptation of this microbe to environmental extremes at the whole-genome level, as well as how to optimally use the strain in bioremediation of chemically polluted sites.
PB - Springer
T2 - Extremophiles
T1 - High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals
VL - 23
IS - 4
SP - 399
EP - 405
DO - 10.1007/s00792-019-01092-w
ER -
@article{
author = "Izrael‑Živković, Lidija and Beškoski, Vladimir and Rikalović, Milena and Kazazić, Snježana and Shapiro, Nicole and Woyke, Tanja and Gojgić-Cvijović, Gordana and Vrvić, Miroslav and Maksimović, Nela and Karadžić, Ivanka",
year = "2019",
abstract = "The strain Pseudomonas aeruginosa san ai, isolated from an extreme environment (industrial mineral cutting oil, pH 10), is able to survive and persist in the presence of a variety of pollutants such as heavy metals and organic chemicals. The genome of P. aeruginosa san ai is 6.98 Mbp long with a GC content of 66.08% and 6485 protein encoding genes. A large number of genes associated with proteins, responsible for microbial resistance to heavy metal ions andinvolved in catabolism of toxic aromatic organic compounds were identified. P.aeruginosa san ai is a highly cadmium-resistant strain. Proteome analysis of biomass after cadmium exposal confirmed a high tolerance to sublethal concentrations of cadmium (100mg/L), based on: extracellular biosorption, bioaccumulation, biofilm formation, controlled siderophore production and a pronounced metalloprotein synthesis. Proteins responsible for survival in osmostress conditions during exposure to elevated concentrations of cadmium (200mg/L) demonstrate a strong genetic potential of P. aeruginosa san ai for survival and adaptation. Sequencing of P. aeruginosa san ai genome provides valuable insights into the evolution and adaptation of this microbe to environmental extremes at the whole-genome level, as well as how to optimally use the strain in bioremediation of chemically polluted sites.",
publisher = "Springer",
journal = "Extremophiles",
title = "High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals",
volume = "23",
number = "4",
pages = "399-405",
doi = "10.1007/s00792-019-01092-w"
}
Izrael‑Živković, L., Beškoski, V., Rikalović, M., Kazazić, S., Shapiro, N., Woyke, T., Gojgić-Cvijović, G., Vrvić, M., Maksimović, N.,& Karadžić, I.. (2019). High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals. in Extremophiles
Springer., 23(4), 399-405.
https://doi.org/10.1007/s00792-019-01092-w
Izrael‑Živković L, Beškoski V, Rikalović M, Kazazić S, Shapiro N, Woyke T, Gojgić-Cvijović G, Vrvić M, Maksimović N, Karadžić I. High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals. in Extremophiles. 2019;23(4):399-405.
doi:10.1007/s00792-019-01092-w .
Izrael‑Živković, Lidija, Beškoski, Vladimir, Rikalović, Milena, Kazazić, Snježana, Shapiro, Nicole, Woyke, Tanja, Gojgić-Cvijović, Gordana, Vrvić, Miroslav, Maksimović, Nela, Karadžić, Ivanka, "High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals" in Extremophiles, 23, no. 4 (2019):399-405,
https://doi.org/10.1007/s00792-019-01092-w . .
, 
