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Expression and Characterization of a Dye-Decolorizing Peroxidase from Pseudomonas Fluorescens Pf0-1
dc.creator | Lončar, Nikola | |
dc.creator | Drašković, Natalija | |
dc.creator | Božić, Nataša | |
dc.creator | Romero, Elvira | |
dc.creator | Simić, Stefan | |
dc.creator | Opsenica, Igor | |
dc.creator | Vujčić, Zoran | |
dc.creator | Fraaije, Marco W. | |
dc.date.accessioned | 2019-07-10T09:29:01Z | |
dc.date.available | 2019-07-10T09:29:01Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 2073-4344 | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/3021 | |
dc.description.abstract | The consumption of dyes is increasing worldwide in line with the increase of population and demand for clothes and other colored products. However, the e ciency of dyeing processes is still poor and results in large amounts of colored e uents. It is desired to develop a portfolio of enzymes which can be used for the treatment of colored wastewaters. Herein, we used genome sequence information to discover a dye-decolorizing peroxidase (DyP) from Pseudomonas fluorescens Pf-01. Two genes putatively encoding for DyPs were identified in the respective genome and cloned for expression in Escherichia coli, of which one (PfDyP B2) could be overexpressed as a soluble protein. PfDyP B2 shows some typical features known for DyPs which includes the ability to convert dyes at the expense of hydrogen peroxide. Interestingly, t-butyl hydroperoxide could be used as an alternative substrate to hydrogen peroxide. Immobilization of PfDyP B2 in calcium-alginate beads resulted in a significant increase in stability: PfDyP B2 retains 80% of its initial activity after 2 h incubation at 50 °C, while the soluble enzyme is inactivated within minutes. PfDyP B2 was also tested with aniline and ethyl diazoacetate as substrates. Based on GC-MS analyses, 30% conversion of the starting material was achieved after 65 h at 30 °C. Importantly, this is the first report of a DyP-catalyzed insertion of a carbene into an N-H bond. | en |
dc.language.iso | en | sr |
dc.publisher | MDPI | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172048/RS// | sr |
dc.rights | openAccess | sr |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Catalysts | sr |
dc.subject | DyP peroxidase | sr |
dc.subject | oxidoreductase | sr |
dc.subject | reactive dye | sr |
dc.subject | decolorization | sr |
dc.title | Expression and Characterization of a Dye-Decolorizing Peroxidase from Pseudomonas Fluorescens Pf0-1 | en |
dc.type | article | sr |
dc.rights.license | BY | sr |
dcterms.abstract | Ромеро, Елвира; Драшковић, Наталија; Лончар, Никола; Вујчић, Зоран; Опсеница, Игор; Фрааије, Марцо W.; Симић, Стефан; Божић, Наташа; | |
dc.rights.holder | Authors | sr |
dc.citation.volume | 9 | |
dc.citation.spage | 463 | |
dc.citation.rank | M22~ | |
dc.identifier.doi | 10.3390/catal9050463 | |
dc.identifier.fulltext | https://cer.ihtm.bg.ac.rs//bitstream/id/7482/catalysts-09-00463.pdf | |
dc.identifier.scopus | 2-s2.0-85066730056 | |
dc.identifier.wos | 000472678500072 | |
dc.type.version | publishedVersion | sr |