Changes in electrophoresis pattern after interaction of supercoiled plasmid pBR322 DNA with avarol was studied at a micromolar concentration of reactants under mild reaction conditions. Interactions of avarol with linear high-molecular CT-DNA at millimolar concentrations were analyzed by electrophoresis and UV spectrophotometry. It was observed that avarol is capable of quenching ethidium bromide fluorescence in DNA bands. An increase in the absorbance of DNA was detected. The results indicate the binding of avarol to DNA and/or modification of nucleotide bases.
Proučavane su promene elektroforetskog ponašanja DNA posle interakcija superhelikoidalnog plazmida pBR322 s avarolom pri mikromolarnim koncentracijama reaktanata pod blagim reakcionim uslovima. Interakcije avarola sa linearnom visokomolekulskom CT-DNA pri milimolarnim koncentracijama analizirane su elektroforezom i UV spektrofotometrijom. Uočeno je da je avarol u stanju da gasi fluorescenciju etidijum-bromida u trakama koje potiču od DNA. Detektovan je porast apsorbancije DNA. Rezultati ukazuju na vezivanje avarona za DNA i/ili modifikaciju nukleotidnih baza.
Keywords:
avarol / hydroquinone / anti-tumor activity / pBR322 DNA / calf thymus DNA
Source:
Journal of the Serbian Chemical Society, 2007, 72, 12, 1265-1269
TY - JOUR
AU - Vujčić, Miroslava
AU - Tufegdžić, Srđan
AU - Vujčić, Zoran
AU - Gašić, Miroslav J.
AU - Sladić, Dušan
PY - 2007
UR - https://cer.ihtm.bg.ac.rs/handle/123456789/302
AB - Changes in electrophoresis pattern after interaction of supercoiled plasmid pBR322 DNA with avarol was studied at a micromolar concentration of reactants under mild reaction conditions. Interactions of avarol with linear high-molecular CT-DNA at millimolar concentrations were analyzed by electrophoresis and UV spectrophotometry. It was observed that avarol is capable of quenching ethidium bromide fluorescence in DNA bands. An increase in the absorbance of DNA was detected. The results indicate the binding of avarol to DNA and/or modification of nucleotide bases.
AB - Proučavane su promene elektroforetskog ponašanja DNA posle interakcija superhelikoidalnog plazmida pBR322 s avarolom pri mikromolarnim koncentracijama reaktanata pod blagim reakcionim uslovima. Interakcije avarola sa linearnom visokomolekulskom CT-DNA pri milimolarnim koncentracijama analizirane su elektroforezom i UV spektrofotometrijom. Uočeno je da je avarol u stanju da gasi fluorescenciju etidijum-bromida u trakama koje potiču od DNA. Detektovan je porast apsorbancije DNA. Rezultati ukazuju na vezivanje avarona za DNA i/ili modifikaciju nukleotidnih baza.
PB - Serbian Chemical Society
T2 - Journal of the Serbian Chemical Society
T1 - Interactions of the anti-tumor sesquiterpene hydroquinone avarol with DNA in vitro
T1 - Interakcije antitumorskog seskviterpenskog hidrohinona avarola sa DNA in vitro
VL - 72
IS - 12
SP - 1265
EP - 1269
DO - 10.2298/JSC0712265V
ER -
@article{
author = "Vujčić, Miroslava and Tufegdžić, Srđan and Vujčić, Zoran and Gašić, Miroslav J. and Sladić, Dušan",
year = "2007",
abstract = "Changes in electrophoresis pattern after interaction of supercoiled plasmid pBR322 DNA with avarol was studied at a micromolar concentration of reactants under mild reaction conditions. Interactions of avarol with linear high-molecular CT-DNA at millimolar concentrations were analyzed by electrophoresis and UV spectrophotometry. It was observed that avarol is capable of quenching ethidium bromide fluorescence in DNA bands. An increase in the absorbance of DNA was detected. The results indicate the binding of avarol to DNA and/or modification of nucleotide bases., Proučavane su promene elektroforetskog ponašanja DNA posle interakcija superhelikoidalnog plazmida pBR322 s avarolom pri mikromolarnim koncentracijama reaktanata pod blagim reakcionim uslovima. Interakcije avarola sa linearnom visokomolekulskom CT-DNA pri milimolarnim koncentracijama analizirane su elektroforezom i UV spektrofotometrijom. Uočeno je da je avarol u stanju da gasi fluorescenciju etidijum-bromida u trakama koje potiču od DNA. Detektovan je porast apsorbancije DNA. Rezultati ukazuju na vezivanje avarona za DNA i/ili modifikaciju nukleotidnih baza.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Interactions of the anti-tumor sesquiterpene hydroquinone avarol with DNA in vitro, Interakcije antitumorskog seskviterpenskog hidrohinona avarola sa DNA in vitro",
volume = "72",
number = "12",
pages = "1265-1269",
doi = "10.2298/JSC0712265V"
}
Vujčić, M., Tufegdžić, S., Vujčić, Z., Gašić, M. J.,& Sladić, D.. (2007). Interactions of the anti-tumor sesquiterpene hydroquinone avarol with DNA in vitro. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 72(12), 1265-1269.
https://doi.org/10.2298/JSC0712265V
Vujčić M, Tufegdžić S, Vujčić Z, Gašić MJ, Sladić D. Interactions of the anti-tumor sesquiterpene hydroquinone avarol with DNA in vitro. in Journal of the Serbian Chemical Society. 2007;72(12):1265-1269.
doi:10.2298/JSC0712265V .
Vujčić, Miroslava, Tufegdžić, Srđan, Vujčić, Zoran, Gašić, Miroslav J., Sladić, Dušan, "Interactions of the anti-tumor sesquiterpene hydroquinone avarol with DNA in vitro" in Journal of the Serbian Chemical Society, 72, no. 12 (2007):1265-1269,
https://doi.org/10.2298/JSC0712265V . .
