Show simple item record

dc.creatorĐurić, Aleksandra
dc.creatorGojgić-Cvijović, Gordana
dc.creatorJakovljević, Dragica
dc.creatorKekez, Branka
dc.creatorKojic, Jovana Stefanovic
dc.creatorMattinen, Maija-Liisa
dc.creatorHarju, Inka Elina
dc.creatorVrvić, Miroslav
dc.creatorBeškoski, Vladimir
dc.date.accessioned2019-07-09T13:39:03Z
dc.date.available2018-06-08
dc.date.issued2017
dc.identifier.issn0141-8130
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/3017
dc.description.abstractVarious microorganisms isolated from polluted environments, such as Pseudomonas sp. and Micrococcus sp. can synthesize exopolysaccharides (EPSs) which are natural, non-toxic and biodegradable polymers. EPSs play a key role in protection of microbial cells under various external influences. For humans, these substances have potential use in many industries. EPSs can be applied as a flavor or a fragrance carrier, an emulsifier, a stabilizer, a prebiotic, an antioxidant or an antitumor agent. In this study, we characterized an environmental microorganism that produces EPS, optimized EPS production by this strain and characterized the EPS produced. Isolate CH-KOV3 was identified as Brachybacterium paraconglomeratum. The sucrose level in the growth medium greatly influenced EPS production, and the highest yield was when the microorganism was incubated in media with 500 g/L, of sucrose. The optimal temperature and pH were 28 degrees C and 7.0, respectively. The nuclear magnetic resonance (NMR) results and GC MS analysis confirmed that the residues were D-fructofuranosyl residues with beta-configuration, where fructose units are linked by beta-2,6-glycosidic bonds, with beta-2,1-linked branches. All these data indicate that the investigated EPS is a levan-type polysaccharide. Thus, it was concluded that Brachybacterium sp. CH-KOV3 could constitute a new source for production of the bioactive polysaccharide, levan.en
dc.publisherElsevier Science Bv, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43004/RS//
dc.relationFCUB ERA
dc.relationJapan International Cooperation Agency (JICA)
dc.rightsembargoedAccess
dc.sourceInternational Journal of Biological Macromolecules
dc.subjectPolluted environmentsen
dc.subjectExopolysaccharidesen
dc.subjectBrachybacterium paraconglomeratumen
dc.titleBrachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levanen
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractБескоски, Владимир П.; Ђурић, Aлександра; Гојгић Цвијовић, Гордана; Којиц, Јована Стефановиц; Маттинен, Маија-Лииса; Харју, Инка Елина; Јаковљевић, Драгица; Кекез, Бранка; Врвиц, Мирослав М.;
dc.citation.volume104
dc.citation.spage311
dc.citation.epage321
dc.citation.other104: 311-321
dc.citation.rankM21
dc.description.otherThis is peer-reviewed version of the article: Aleksandra Djurić, Gordana Gojgić-Cvijović, Dragica Jakovljević, Branka Kekez, Jovana Stefanović Kojić, Maija-Liisa Mattinen, Inka Elina Harju, Miroslav M.Vrvić, Vladimir P.Beškoski, Brachybacterium sp.CH-KOV3 isolated from an oil-polluted environment–a new producer of levan, International Journal of Biological Macromolecules, 2017, 104, 311-321, [http://dx.doi.org/10.1016/j.ijbiomac.2017.06.034]
dc.description.other[http://cer.ihtm.bg.ac.rs/handle/123456789/2097]
dc.identifier.pmid28602989
dc.identifier.doi10.1016/j.ijbiomac.2017.06.034
dc.identifier.rcubConv_3815
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs/bitstream/id/7442/18_0907_10.1016j.ijbiomac.2017.06.034.pdf
dc.identifier.scopus2-s2.0-85020640842
dc.identifier.wos000412959300035
dc.type.versionacceptedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record