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Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan

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2017
18_0907_10.1016j.ijbiomac.2017.06.034.pdf (1.306Mb)
Authors
Đurić, Aleksandra
Gojgić-Cvijović, Gordana
Jakovljević, Dragica
Kekez, Branka
Kojic, Jovana Stefanovic
Mattinen, Maija-Liisa
Harju, Inka Elina
Vrvić, Miroslav
Beškoski, Vladimir
Article (Accepted Version)
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Abstract
Various microorganisms isolated from polluted environments, such as Pseudomonas sp. and Micrococcus sp. can synthesize exopolysaccharides (EPSs) which are natural, non-toxic and biodegradable polymers. EPSs play a key role in protection of microbial cells under various external influences. For humans, these substances have potential use in many industries. EPSs can be applied as a flavor or a fragrance carrier, an emulsifier, a stabilizer, a prebiotic, an antioxidant or an antitumor agent. In this study, we characterized an environmental microorganism that produces EPS, optimized EPS production by this strain and characterized the EPS produced. Isolate CH-KOV3 was identified as Brachybacterium paraconglomeratum. The sucrose level in the growth medium greatly influenced EPS production, and the highest yield was when the microorganism was incubated in media with 500 g/L, of sucrose. The optimal temperature and pH were 28 degrees C and 7.0, respectively. The nuclear magnetic resonance (NM...R) results and GC MS analysis confirmed that the residues were D-fructofuranosyl residues with beta-configuration, where fructose units are linked by beta-2,6-glycosidic bonds, with beta-2,1-linked branches. All these data indicate that the investigated EPS is a levan-type polysaccharide. Thus, it was concluded that Brachybacterium sp. CH-KOV3 could constitute a new source for production of the bioactive polysaccharide, levan.

Keywords:
Polluted environments / Exopolysaccharides / Brachybacterium paraconglomeratum
Source:
International Journal of Biological Macromolecules, 2017, 104, 311-321
Publisher:
  • Elsevier Science Bv, Amsterdam
Projects:
  • Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-43004)
  • FCUB ERA
  • Japan International Cooperation Agency (JICA)
Note:
  • This is peer-reviewed version of the article: Aleksandra Djurić, Gordana Gojgić-Cvijović, Dragica Jakovljević, Branka Kekez, Jovana Stefanović Kojić, Maija-Liisa Mattinen, Inka Elina Harju, Miroslav M.Vrvić, Vladimir P.Beškoski, Brachybacterium sp.CH-KOV3 isolated from an oil-polluted environment–a new producer of levan, International Journal of Biological Macromolecules, 2017, 104, 311-321, http://dx.doi.org/10.1016/j.ijbiomac.2017.06.034
  • http://cer.ihtm.bg.ac.rs/handle/123456789/2097

DOI: 10.1016/j.ijbiomac.2017.06.034

ISSN: 0141-8130

PubMed: 28602989

WoS: 000412959300035

Scopus: 2-s2.0-85020640842
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URI
http://cer.ihtm.bg.ac.rs/handle/123456789/3017
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