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dc.creatorAnđelković, Uroš
dc.creatorTufegdžić, Srđan
dc.creatorPopović, Milica
dc.date.accessioned2019-07-01T06:24:48Z
dc.date.available2018-09-14
dc.date.issued2017
dc.identifier.issn0173-0835
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/2985
dc.description.abstractThe exclusive properties of monolithic supports enable fast mass transfer, high porosity, low back pressure, easy preparation process and miniaturisation, and the availability of different chemistries make them particularly suitable materials for high-throughput (HTP) protein and peptide separation. In this review recent advances in monolith-based chromatographic supports for HTP screening of protein and peptide samples are presented and their application in HTP sample preparation (separation, enrichment, depletion, proteolytic digestion) for HTP proteomics is discussed. Development and applications of different monolithic capillary columns in HTP MS-based bottom-up and top-down proteomics are overviewed. By discussing the chromatographic conditions and the mass spectrometric data acquisition conditions an attempt is made to present currently demonstrated capacities of monolithic capillary columns for HTP identification and quantification of proteins and peptides from complex biological samples by MS-based proteomics. Some recent advances in basic monolith technology of importance for proteomics are also discussed.en
dc.publisherWiley, Hoboken
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172048/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172055/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/324400/EU//
dc.rightsembargoedAccess
dc.sourceElectrophoresis
dc.subjectHigh-throughputen
dc.subjectLiquid chromatographyen
dc.subjectMass spectrometryen
dc.subjectMonolithsen
dc.subjectProteomicsen
dc.titleUse of monolithic supports for high-throughput protein and peptide separation in proteomicsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractТуфегджић, Срђан; Aнђелковић, Урош; Поповиц, Милица;
dc.citation.volume38
dc.citation.issue22-23
dc.citation.spage2851
dc.citation.epage2869
dc.citation.other38(22-23): 2851-2869
dc.citation.rankM22
dc.description.otherThis is the peer-reviewed version of the following article:Andjelkovic, U.; Tufegdzic, S.; Popović, M. Use of Monolithic Supports for High-Throughput Protein and Peptide Separation in Proteomics. Electrophoresis 2017, 38 (22–23), 2851–2869, [https://dx.doi.org/10.1002/elps.201700260]
dc.description.other[http://cer.ihtm.bg.ac.rs/handle/123456789/2122]
dc.identifier.pmid28906564
dc.identifier.doi10.1002/elps.201700260
dc.identifier.rcubConv_3830
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs/bitstream/id/7325/Andjelkovi-_et_al-2017-ELECTROPHORESIS.pdf
dc.identifier.scopus2-s2.0-85036597546
dc.identifier.wos000416867300006
dc.type.versionacceptedVersion


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