Characterization and effects of binding of food-derived bioactive phycocyanobilin to bovine serum albumin
Authors
Minic, SimeonStanić-Vučinić, Dragana

Radomirović, Mirjana

Radibratović, Milica

Milčić, Miloš

Nikolić, Milan

Ćirković Veličković, Tanja

Article (Accepted Version)
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Show full item recordAbstract
Phycocyanobilin (PCB) is a blue tetrapyrrole chromophore of C-phycocyanin, the main protein of the microalga Spirulina, with numerous proven health-related benefits. We examined binding of PCB to bovine serum albumin (BSA) and how it affects protein and ligand stability. Protein fluorescence quenching and microscale thermophoresis demonstrated high-affinity binding (K-a = 2 x 10(6) M-1). Spectroscopic titration with molecular docking analysis revealed two binding sites on BSA, at the inter-domain cleft and at subdomain IB, while CD spectroscopy indicated stereo-selective binding of the P conformer of the pigment to the protein. The PCB protein complex showed increased thermal stability. Although complex formation partly masked the antioxidant properties of PCB and BSA, a mutually protective effect against free radical-induced oxidation was found. BSA could be suitable for delivery of PCB as a food colorant or bioactive component. Our results also highlight subtle differences between PC...B binding to bovine vs. human serum albumin.
Keywords:
Spirulina / Phycocyanobilin / Bovine serum albumin / Binding / Stability / AntioxidantSource:
Food Chemistry, 2018, 239, 1090-1099Publisher:
- Elsevier Sci Ltd, Oxford
Projects:
- Molecular properties and modifications of some respiratory and nutritional allergens (RS-172024)
- Reinforcement of the Faculty of Chemistry, University of Belgrade, towards becoming a Center of Excellence in the region of WB for Molecular Biotechnology and Food research (EU-256716)
Note:
- This is peer-reviewed version of the following article: Minic, S.; Stanic-Vucinic, D.; Radomirovic, M.; Radibratovic, M.; Milcic, M.; Nikolic, M.; Cirkovic Velickovic, T. Characterization and Effects of Binding of Food-Derived Bioactive Phycocyanobilin to Bovine Serum Albumin. Food Chemistry 2018, 239, 1090–1099, https://doi.org/10.1016/j.foodchem.2017.07.066
- http://cer.ihtm.bg.ac.rs/handle/123456789/2478
DOI: 10.1016/j.foodchem.2017.07.066
ISSN: 0308-8146
PubMed: 28873526