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Biodegradation of poly(ε-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil

Authorized Users Only
2019
Authors
Mandic, Mina
Spasic, Jelena
Ponjavic, Marijana
Nikolic, Marija S.
Ćosović, Vladan
O'Connor, Kevin E.
Nikodinović-Runić, Jasmina
Đokić, Lidija
Jeremić, Sanja
Article (Published version)
,
Elsevier BV
Metadata
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Abstract
Petrochemical plastics are generally recalcitrant to microbial degradation and accumulate in the environment. Biodegradable polymers obtained synthetically like poly(ε-caprolactone) (PCL) or polyhydroxyalkanoates (PHA), obtained biotechnologically, have shown great potential as a replacement for petroleum-based plastics. Nevertheless, their biodegradation and environmental faith have been less examined. In this study, thin films of PCL (200 μm) and medium chain length PHA (mcl-PHA, 70 M fraction of 3-hydroxyoctanoate and 30 M fraction of 3-hydroxydecanoate, 600 μm) were exposed to total protein preparations (extracellular proteins combined with a crude cell extract) of soil isolates Pseudomonas chlororaphis B-561 and Streptomyces sp. BV315 that had been grown on waste cooking oil as a sole carbon source. Biodegradation potential of two polyesters was evaluated in buffer with total protein preparations and in a laboratory compost model system augmented with selected bacteria. Overall, P...CL showed better biodegradation properties in comparison to mcl-PHA. Both materials showed surface erosion after 4-weeks of exposure to total protein preparations of both strains, with a moderate weight loss of 1.3% when P. chlororaphis B-561 was utilized. In laboratory compost model system PCL and mcl-PHA showed significant weight loss ranging from 13 to 17% when Streptomyces sp. BV315 culture was used. Similar weight loss of PCL and mcl-PHA was achieved for 4 and 8 weeks, respectively indicating slower degradation of mcl-PHA. Growth on waste cooking oil as a sole carbon source increased the potential of both tested strains to degrade PCL and mcl-PHA, making them good candidates for augmentation of compost cultures in waste management of both waste cooking oils and biodegradable polymers.

Keywords:
Biopolymers / Enzymes / Pseudomonas / Streptomyces / Biodegradation / Compost
Source:
Polymer Degradation and Stability, 2019, 162, 160-168
Publisher:
  • Elsevier BV
Projects:
  • Microbial diversity study and characterization of beneficial environmental microorganisms (RS-173048)
  • Synthesis and characterization of novel functional polymers and polymeric nanocomposites (RS-172062)
Note:
  • Peeer-reviewed version: http://cer.ihtm.bg.ac.rs/handle/123456789/2956

DOI: 10.1016/j.polymdegradstab.2019.02.012

ISSN: 0141-3910

WoS: 000465055500019

Scopus: 2-s2.0-85062047760
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URI
http://cer.ihtm.bg.ac.rs/handle/123456789/2955
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