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Omics methods as a tool for investigation of food allergies

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2017
0406_3.pdf (1.228Mb)
Authors
Anđelković, Uroš
Gavrović-Jankulović, Marija
Martinovic, Tamara
Josić, Djuro
Article (Accepted Version)
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Abstract
Use of foodomics, mostly proteomic and genomic based methods, for study of allergens in food is presented. Immunological methods and nucleic acid-based methods are still most frequently used for diagnosis of allergies and for qualitative and quantitative determination of food allergens. They are sensitive, and can be used for the determination of allergens in trace concentrations. However, lack of specificity and cross-reaction of some antibodies can still be a relevant source of bias. The epitopes of protein allergens with posttranslational modifications and their changes originated during food processing cannot be traced by use of nucleic acid-based strategies. Recent developments of both antibody and nucleic acid-based biosensors, their miniaturization and increasing application of nanotechnology, significantly supported further use of both strategies. Regarding accuracy, reliability and sensitivity, mass spectrometry-based methods bring important advantage over both above presented... strategies. Furthermore, the increasing use of mass spectrometry (MS) is discussed. Combined with proper sample preparation, liquid chromatography (LC) and/or different electrophoretic methods, targeted approach in mass spectrometry-based allergen analysis brings an additional strategic advance. However, MS is still rarely used for high-throughput analyses and detection and quantification of allergens for the reasons of price and relatively long time necessary for analysis. Recent developments of new high-resolution instruments are encouraging and enable development in the direction of a high-throughput strategy. Consequently, fast, very sensitive, reliable and accurate detection and quantification of allergens in highly complex samples such as food matrices, and the use of MS in routine determination of allergens can be reached in near future.

Keywords:
Proteomics / Allergen / Food allergy / Mass spectrometry / Biosensors
Source:
Trac-Trends in Analytical Chemistry, 2017, 96, 107-115
Publisher:
  • Elsevier Sci Ltd, Oxford
Projects:
  • Production, purification and characterization of enzymes and small molecules and their application as soluble or immobilized in food biotechnology, biofuels production and environmental protection (RS-172048)
  • Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance (RS-172049)
  • Interactions of natural products, their derivatives and coordination compounds with proteins and nucleic acids (RS-172055)
  • University of Rijeka [13.11.1.2.03]
  • Ministry of Sciences of Croatia
  • COST action [FA1402 ImpARAS]
Note:
  • This is a peer-reviewed version of article: https://doi.org/10.1016/j.trac.2017.07.011
  • http://cer.ihtm.bg.ac.rs/handle/123456789/2113

DOI: 10.1016/j.trac.2017.07.011

ISSN: 0165-9936

WoS: 000415910300012

Scopus: 2-s2.0-85026556738
[ Google Scholar ]
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URI
http://cer.ihtm.bg.ac.rs/handle/123456789/2937
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  • Radovi istraživača / Researchers' publications
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IHTM

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