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Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium

Authorized Users Only
2019
Authors
Gojgić-Cvijović, Gordana
Jakovljević, Dragica
Lončarević, Branka
Todorović, Nina
Pergal, Marija
Ciric, J.
Loos, K.
Beškoski, Vladimir
Vrvić, Miroslav
Article (Published version)
,
Elsevier B.V.
Metadata
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Abstract
The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100-140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 532 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 x 10(6) Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium... with 200 g/L sucrose.

Keywords:
Levan / Molasses / Molecular weight
Source:
International Journal of Biological Macromolecules, 2019, 121, 142-151
Publisher:
  • Elsevier
Funding / projects:
  • Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-43004)
  • Natural products of wild, cultivated and edible plants: structure and bioactivity determination (RS-172053)
  • Synthesis and characterization of novel functional polymers and polymeric nanocomposites (RS-172062)
Note:
  • Peer-reviewed version: http://cer.ihtm.bg.ac.rs/handle/123456789/2503

DOI: 10.1016/j.ijbiomac.2018.10.019

ISSN: 0141-8130

PubMed: 30291934

WoS: 000452346100018

Scopus: 2-s2.0-85054437248
[ Google Scholar ]
36
26
URI
https://cer.ihtm.bg.ac.rs/handle/123456789/2926
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - JOUR
AU  - Gojgić-Cvijović, Gordana
AU  - Jakovljević, Dragica
AU  - Lončarević, Branka
AU  - Todorović, Nina
AU  - Pergal, Marija
AU  - Ciric, J.
AU  - Loos, K.
AU  - Beškoski, Vladimir
AU  - Vrvić, Miroslav
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2926
AB  - The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100-140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 532 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 x 10(6) Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium with 200 g/L sucrose.
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium
VL  - 121
SP  - 142
EP  - 151
DO  - 10.1016/j.ijbiomac.2018.10.019
ER  - 
@article{
author = "Gojgić-Cvijović, Gordana and Jakovljević, Dragica and Lončarević, Branka and Todorović, Nina and Pergal, Marija and Ciric, J. and Loos, K. and Beškoski, Vladimir and Vrvić, Miroslav",
year = "2019",
abstract = "The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100-140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 532 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 x 10(6) Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium with 200 g/L sucrose.",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium",
volume = "121",
pages = "142-151",
doi = "10.1016/j.ijbiomac.2018.10.019"
}
Gojgić-Cvijović, G., Jakovljević, D., Lončarević, B., Todorović, N., Pergal, M., Ciric, J., Loos, K., Beškoski, V.,& Vrvić, M.. (2019). Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium. in International Journal of Biological Macromolecules
Elsevier., 121, 142-151.
https://doi.org/10.1016/j.ijbiomac.2018.10.019
Gojgić-Cvijović G, Jakovljević D, Lončarević B, Todorović N, Pergal M, Ciric J, Loos K, Beškoski V, Vrvić M. Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium. in International Journal of Biological Macromolecules. 2019;121:142-151.
doi:10.1016/j.ijbiomac.2018.10.019 .
Gojgić-Cvijović, Gordana, Jakovljević, Dragica, Lončarević, Branka, Todorović, Nina, Pergal, Marija, Ciric, J., Loos, K., Beškoski, Vladimir, Vrvić, Miroslav, "Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium" in International Journal of Biological Macromolecules, 121 (2019):142-151,
https://doi.org/10.1016/j.ijbiomac.2018.10.019 . .

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