Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium
Само за регистроване кориснике
2019
Аутори
Gojgić-Cvijović, GordanaJakovljević, Dragica
Lončarević, Branka
Todorović, Nina
Pergal, Marija
Ciric, J.
Loos, K.
Beškoski, Vladimir
Vrvić, Miroslav
Чланак у часопису (Објављена верзија)
,
Elsevier B.V.
Метаподаци
Приказ свих података о документуАпстракт
The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100-140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 532 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 x 10(6) Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium... with 200 g/L sucrose.
Кључне речи:
Levan / Molasses / Molecular weightИзвор:
International Journal of Biological Macromolecules, 2019, 121, 142-151Издавач:
- Elsevier
Финансирање / пројекти:
- Симултана биоремедијација и соилификација деградираних простора, за очување природних ресурса биолошки активних супстанци и развој и производњу биоматеријала и дијететских производа (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43004)
- Биоактивни природни производи самониклих, гајених и јестивих биљака: одређивање структура и активности (RS-MESTD-Basic Research (BR or ON)-172053)
- Синтеза и карактеризација нових функционалних полимера и полимерних нанокомпозита (RS-MESTD-Basic Research (BR or ON)-172062)
Напомена:
- Peer-reviewed version: http://cer.ihtm.bg.ac.rs/handle/123456789/2503
DOI: 10.1016/j.ijbiomac.2018.10.019
ISSN: 0141-8130
PubMed: 30291934
WoS: 000452346100018
Scopus: 2-s2.0-85054437248
Институција/група
IHTMTY - JOUR AU - Gojgić-Cvijović, Gordana AU - Jakovljević, Dragica AU - Lončarević, Branka AU - Todorović, Nina AU - Pergal, Marija AU - Ciric, J. AU - Loos, K. AU - Beškoski, Vladimir AU - Vrvić, Miroslav PY - 2019 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/2926 AB - The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100-140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 532 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 x 10(6) Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium with 200 g/L sucrose. PB - Elsevier T2 - International Journal of Biological Macromolecules T1 - Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium VL - 121 SP - 142 EP - 151 DO - 10.1016/j.ijbiomac.2018.10.019 ER -
@article{ author = "Gojgić-Cvijović, Gordana and Jakovljević, Dragica and Lončarević, Branka and Todorović, Nina and Pergal, Marija and Ciric, J. and Loos, K. and Beškoski, Vladimir and Vrvić, Miroslav", year = "2019", abstract = "The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100-140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 532 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 x 10(6) Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium with 200 g/L sucrose.", publisher = "Elsevier", journal = "International Journal of Biological Macromolecules", title = "Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium", volume = "121", pages = "142-151", doi = "10.1016/j.ijbiomac.2018.10.019" }
Gojgić-Cvijović, G., Jakovljević, D., Lončarević, B., Todorović, N., Pergal, M., Ciric, J., Loos, K., Beškoski, V.,& Vrvić, M.. (2019). Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium. in International Journal of Biological Macromolecules Elsevier., 121, 142-151. https://doi.org/10.1016/j.ijbiomac.2018.10.019
Gojgić-Cvijović G, Jakovljević D, Lončarević B, Todorović N, Pergal M, Ciric J, Loos K, Beškoski V, Vrvić M. Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium. in International Journal of Biological Macromolecules. 2019;121:142-151. doi:10.1016/j.ijbiomac.2018.10.019 .
Gojgić-Cvijović, Gordana, Jakovljević, Dragica, Lončarević, Branka, Todorović, Nina, Pergal, Marija, Ciric, J., Loos, K., Beškoski, Vladimir, Vrvić, Miroslav, "Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium" in International Journal of Biological Macromolecules, 121 (2019):142-151, https://doi.org/10.1016/j.ijbiomac.2018.10.019 . .