Show simple item record

dc.creatorBeškoski, Vladimir
dc.creatorMiletić, Srđan
dc.creatorIlić, Mila
dc.creatorGojgić-Cvijović, Gordana
dc.creatorPapić, Petar
dc.creatorMarić, Nenad
dc.creatorŠolević Knudsen, Tatjana
dc.creatorJovančićević, Branimir
dc.creatorNakano, Takeshi
dc.creatorVrvić, Miroslav
dc.date.accessioned2019-05-31T07:55:00Z
dc.date.available2017-12-06
dc.date.issued2017
dc.identifier.issn1863-0650
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/2925
dc.description.abstractThe objective of this study was to stimulate microbial biodegradation of petroleum pollution in groundwater and to analyze changes in the abundance and distribution of organic compounds detectable in petroleum. Bioremediation was conducted in a closed bipolar system, by bioaugmentation with consortia of hydrocarbon degrading microorganisms (HD) and biostimulation with nutrients. Comprehensive twodimensional gas chromatography-mass spectrometry (GC X GC-MS) was used to visualize all fractions simultaneously. During the study, the content of total petroleum hydrocarbon (TPH) in groundwater decreased by 92.7% of the initial level, and the average rate of biodegradation was 0.1 mg/L per day. Increased numbers of HD were observed and the dominant genera were Pseudomonas, Rhodococcus, Achromobacter, Bacillus, and Micromonospora. In the first 30 days of bioremediation, there was no significant biodegradation of n-alkanes and petroleum biomarkers - isoprenoids such as pristane and phytane, and polycyclic-saturated hydrocarbons such as terpanes and steranes. However, after 60 days of bioremediation, more than 95% of n-alkanes, terpanes and steranes were biodegraded. Phenanthrene and its methyl-,dimethyl-, and trimethyl-isomers were biodegraded and reduced by more than 99% of their initial levels. However, their decomposition had clearly commenced after just 30 days. This is a somewhat surprising result since it follows that the phenanthrenes were more susceptible to biodegradation than the n-alkanes and isoprenoids. Depending on the microbial community used for bioaugmentation, biodegradation of phenanthrene can precede biodegradation of saturated hydrocarbons.
dc.publisherWiley, Hoboken
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43004/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/176006/RS//
dc.relationJapan International Cooperation Agency (JICA)
dc.rightsembargoedAccess
dc.sourceClean - Soil, Air, Water
dc.subjectClosed bipolar systems
dc.subjectPetroleum biomarkers
dc.subjectPolycyclic aromatic hydrocarbons
dc.subjectTwo-dimensional gas chromatography–mass spectrometry
dc.subjectZymogenous microorganisms
dc.titleBiodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractНакано, Такесхи; Јованчићевић, Бранимир; Марић, Ненад; Милетић, Срђан Б.; Шолевић-Кнудсен, Т.; Папић, Петар; Илић, Мила В.; Бешкоски, Владимир; Гојгић Цвијовић, Гордана; Врвић, Мирослав М.;
dc.citation.volume45
dc.citation.issue2
dc.citation.rankM22
dc.description.otherThis is the peer-reviewed version of the following article: Beškoski, V. P.; Miletić, S.; Ilić, M.; Gojgić-Cvijović, G.; Papić, P.; Marić, N.; Šolević-Knudsen, T.; Jovančićević, B. S.; Nakano, T.; Vrvić, M. M. Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater. Clean - Soil, Air, Water 2017, 45 (2). [https://doi.org/10.1002/clen.201600023]
dc.description.other[http://cer.ihtm.bg.ac.rs/handle/123456789/2183]
dc.identifier.doi10.1002/clen.201600023
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs/bitstream/id/7104/bitstream_7104.pdf
dc.identifier.scopus2-s2.0-85010284829
dc.identifier.wos000397036000010
dc.type.versionacceptedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record