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Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater

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Authors
Beškoski, Vladimir
Miletić, Srđan
Ilić, Mila
Gojgić-Cvijović, Gordana
Papić, Petar
Marić, Nenad
Šolević Knudsen, Tatjana
Jovančićević, Branimir
Nakano, Takeshi
Vrvić, Miroslav
Article (Accepted Version)
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Abstract
The objective of this study was to stimulate microbial biodegradation of petroleum pollution in groundwater and to analyze changes in the abundance and distribution of organic compounds detectable in petroleum. Bioremediation was conducted in a closed bipolar system, by bioaugmentation with consortia of hydrocarbon degrading microorganisms (HD) and biostimulation with nutrients. Comprehensive twodimensional gas chromatography-mass spectrometry (GC X GC-MS) was used to visualize all fractions simultaneously. During the study, the content of total petroleum hydrocarbon (TPH) in groundwater decreased by 92.7% of the initial level, and the average rate of biodegradation was 0.1 mg/L per day. Increased numbers of HD were observed and the dominant genera were Pseudomonas, Rhodococcus, Achromobacter, Bacillus, and Micromonospora. In the first 30 days of bioremediation, there was no significant biodegradation of n-alkanes and petroleum biomarkers - isoprenoids such as pristane and phytane, and... polycyclic-saturated hydrocarbons such as terpanes and steranes. However, after 60 days of bioremediation, more than 95% of n-alkanes, terpanes and steranes were biodegraded. Phenanthrene and its methyl-,dimethyl-, and trimethyl-isomers were biodegraded and reduced by more than 99% of their initial levels. However, their decomposition had clearly commenced after just 30 days. This is a somewhat surprising result since it follows that the phenanthrenes were more susceptible to biodegradation than the n-alkanes and isoprenoids. Depending on the microbial community used for bioaugmentation, biodegradation of phenanthrene can precede biodegradation of saturated hydrocarbons.

Keywords:
Closed bipolar systems / Petroleum biomarkers / Polycyclic aromatic hydrocarbons / Two-dimensional gas chromatography–mass spectrometry / Zymogenous microorganisms
Source:
Clean - Soil, Air, Water, 2017, 45, 2
Publisher:
  • Wiley, Hoboken
Funding / projects:
  • Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-43004)
  • Geochemical investigations of sedimentary rocks - fossil fuels and environmental pollutants (RS-176006)
  • Japan International Cooperation Agency (JICA)
Note:
  • This is the peer-reviewed version of the following article: Beškoski, V. P.; Miletić, S.; Ilić, M.; Gojgić-Cvijović, G.; Papić, P.; Marić, N.; Šolević-Knudsen, T.; Jovančićević, B. S.; Nakano, T.; Vrvić, M. M. Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater. Clean - Soil, Air, Water 2017, 45 (2). https://doi.org/10.1002/clen.201600023
  • http://cer.ihtm.bg.ac.rs/handle/123456789/2183

DOI: 10.1002/clen.201600023

ISSN: 1863-0650

WoS: 000397036000010

Scopus: 2-s2.0-85010284829
[ Google Scholar ]
10
9
URI
https://cer.ihtm.bg.ac.rs/handle/123456789/2925
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - JOUR
AU  - Beškoski, Vladimir
AU  - Miletić, Srđan
AU  - Ilić, Mila
AU  - Gojgić-Cvijović, Gordana
AU  - Papić, Petar
AU  - Marić, Nenad
AU  - Šolević Knudsen, Tatjana
AU  - Jovančićević, Branimir
AU  - Nakano, Takeshi
AU  - Vrvić, Miroslav
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2925
AB  - The objective of this study was to stimulate microbial biodegradation of petroleum pollution in groundwater and to analyze changes in the abundance and distribution of organic compounds detectable in petroleum. Bioremediation was conducted in a closed bipolar system, by bioaugmentation with consortia of hydrocarbon degrading microorganisms (HD) and biostimulation with nutrients. Comprehensive twodimensional gas chromatography-mass spectrometry (GC X GC-MS) was used to visualize all fractions simultaneously. During the study, the content of total petroleum hydrocarbon (TPH) in groundwater decreased by 92.7% of the initial level, and the average rate of biodegradation was 0.1 mg/L per day. Increased numbers of HD were observed and the dominant genera were Pseudomonas, Rhodococcus, Achromobacter, Bacillus, and Micromonospora. In the first 30 days of bioremediation, there was no significant biodegradation of n-alkanes and petroleum biomarkers - isoprenoids such as pristane and phytane, and polycyclic-saturated hydrocarbons such as terpanes and steranes. However, after 60 days of bioremediation, more than 95% of n-alkanes, terpanes and steranes were biodegraded. Phenanthrene and its methyl-,dimethyl-, and trimethyl-isomers were biodegraded and reduced by more than 99% of their initial levels. However, their decomposition had clearly commenced after just 30 days. This is a somewhat surprising result since it follows that the phenanthrenes were more susceptible to biodegradation than the n-alkanes and isoprenoids. Depending on the microbial community used for bioaugmentation, biodegradation of phenanthrene can precede biodegradation of saturated hydrocarbons.
PB  - Wiley, Hoboken
T2  - Clean - Soil, Air, Water
T1  - Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater
VL  - 45
IS  - 2
DO  - 10.1002/clen.201600023
ER  - 
@article{
author = "Beškoski, Vladimir and Miletić, Srđan and Ilić, Mila and Gojgić-Cvijović, Gordana and Papić, Petar and Marić, Nenad and Šolević Knudsen, Tatjana and Jovančićević, Branimir and Nakano, Takeshi and Vrvić, Miroslav",
year = "2017",
abstract = "The objective of this study was to stimulate microbial biodegradation of petroleum pollution in groundwater and to analyze changes in the abundance and distribution of organic compounds detectable in petroleum. Bioremediation was conducted in a closed bipolar system, by bioaugmentation with consortia of hydrocarbon degrading microorganisms (HD) and biostimulation with nutrients. Comprehensive twodimensional gas chromatography-mass spectrometry (GC X GC-MS) was used to visualize all fractions simultaneously. During the study, the content of total petroleum hydrocarbon (TPH) in groundwater decreased by 92.7% of the initial level, and the average rate of biodegradation was 0.1 mg/L per day. Increased numbers of HD were observed and the dominant genera were Pseudomonas, Rhodococcus, Achromobacter, Bacillus, and Micromonospora. In the first 30 days of bioremediation, there was no significant biodegradation of n-alkanes and petroleum biomarkers - isoprenoids such as pristane and phytane, and polycyclic-saturated hydrocarbons such as terpanes and steranes. However, after 60 days of bioremediation, more than 95% of n-alkanes, terpanes and steranes were biodegraded. Phenanthrene and its methyl-,dimethyl-, and trimethyl-isomers were biodegraded and reduced by more than 99% of their initial levels. However, their decomposition had clearly commenced after just 30 days. This is a somewhat surprising result since it follows that the phenanthrenes were more susceptible to biodegradation than the n-alkanes and isoprenoids. Depending on the microbial community used for bioaugmentation, biodegradation of phenanthrene can precede biodegradation of saturated hydrocarbons.",
publisher = "Wiley, Hoboken",
journal = "Clean - Soil, Air, Water",
title = "Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater",
volume = "45",
number = "2",
doi = "10.1002/clen.201600023"
}
Beškoski, V., Miletić, S., Ilić, M., Gojgić-Cvijović, G., Papić, P., Marić, N., Šolević Knudsen, T., Jovančićević, B., Nakano, T.,& Vrvić, M.. (2017). Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater. in Clean - Soil, Air, Water
Wiley, Hoboken., 45(2).
https://doi.org/10.1002/clen.201600023
Beškoski V, Miletić S, Ilić M, Gojgić-Cvijović G, Papić P, Marić N, Šolević Knudsen T, Jovančićević B, Nakano T, Vrvić M. Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater. in Clean - Soil, Air, Water. 2017;45(2).
doi:10.1002/clen.201600023 .
Beškoski, Vladimir, Miletić, Srđan, Ilić, Mila, Gojgić-Cvijović, Gordana, Papić, Petar, Marić, Nenad, Šolević Knudsen, Tatjana, Jovančićević, Branimir, Nakano, Takeshi, Vrvić, Miroslav, "Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater" in Clean - Soil, Air, Water, 45, no. 2 (2017),
https://doi.org/10.1002/clen.201600023 . .

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