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A novel lectin from the sponge Haliclona cratera: isolation, characterization and biological activity

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2002
Authors
Pajic, I
Kljajic, Z
Dogović, Nikola
Sladić, Dušan
Juranić, Zorica
Gašić, Miroslav J.
Article (Published version)
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Abstract
A lectin from the Adriatic sponge Haliclona cratera was purified by ion-exchange and gel chromatography The molecular mass of the lectin is approximately 29 kDa. Purified lectin is rich in hydrophobic and basic amino acids and has an isoelectric point at pH 8.6. H. cratera lectin is relatively heat- and pH-stable. It agglutinates native and trypsinized, papainized and neuraminidase-treated human A, B, O, AB and sheep erythrocytes, and the hemagglutinating activity is independent of Ca2+, Mn2+ and Mg2+ ions; D-galactose and N-acetyl-D-galactosamine are found to be moderate inhibitors of the activity. H. cratera lectin displays cytotoxic effect on HeLa and FemX cells and weak mitogenic effect on human T-lymphocytes pretreated with phytohemagglutinin (PHA). (C) 2002 Elsevier Science Inc. All rights reserved.
Keywords:
sponge / Haliclona cratera / lectin / purification / isolation / stability / cytotoxicity
Source:
Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology, 2002, 132, 2, 213-221
Publisher:
  • Elsevier Science Inc, New York

DOI: 10.1016/S1532-0456(02)00068-6

ISSN: 1532-0456

PubMed: 12106898

WoS: 000177257300010

Scopus: 2-s2.0-0035996841
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URI
http://cer.ihtm.bg.ac.rs/handle/123456789/2837
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IHTM

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