The retention behaviour of some cholic acid derivatives on different adsorbents
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2002
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The thin-layer chromatographic behavior of cholic acid and fourteen of its synthetic derivatives has been studied in six reversed-phase and three normal-phase systems. Four significantly different adsorbents were used - RP-18-silica, CN-silica, polyacrylonitrile adsorbent, and unmodified silica gel. Reversed-phase chromatography was performed with water-organic modifier (methanol, dioxane, or acetone) binary mobile phases of widely variable composition. An approximately linear relationship was obtained between R-M values and the amount of organic modifier in the mobile phases investigated. The effect of mobile-phase composition on retention has been considered and the selectivity of the chromatographic systems is also discussed. Separation mechanisms are proposed on the basis of the results obtained. A positive solvation effect was proposed as a predominant factor determining retention under normal-phase conditions whereas hydrophobic interactions of the substances with non-polar parts... of the adsorbents were assumed to be predominant under reversed-phase conditions.
Ključne reči:
RP-18 silica / CN-silica polyacrylonitrile adsorbent / unmodified silica / retention behavior / cholic acid derivativesIzvor:
Journal of Planar Chromatography: Modern TLC / Thin Layer Chromatography, 2002, 15, 4, 299-305Izdavač:
- Research Inst Medicinal Plants, Budakalasz
DOI: 10.1556/JPC.15.2002.4.11
ISSN: 0933-4173
WoS: 000178677900012
Scopus: 2-s2.0-0036660390
Institucija/grupa
IHTMTY - JOUR AU - Gaica, Sandra AU - Opsenica, Dejan AU - Šolaja, Bogdan AU - Tešić, Živoslav AU - Milojković-Opsenica, Dušanka PY - 2002 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/2834 AB - The thin-layer chromatographic behavior of cholic acid and fourteen of its synthetic derivatives has been studied in six reversed-phase and three normal-phase systems. Four significantly different adsorbents were used - RP-18-silica, CN-silica, polyacrylonitrile adsorbent, and unmodified silica gel. Reversed-phase chromatography was performed with water-organic modifier (methanol, dioxane, or acetone) binary mobile phases of widely variable composition. An approximately linear relationship was obtained between R-M values and the amount of organic modifier in the mobile phases investigated. The effect of mobile-phase composition on retention has been considered and the selectivity of the chromatographic systems is also discussed. Separation mechanisms are proposed on the basis of the results obtained. A positive solvation effect was proposed as a predominant factor determining retention under normal-phase conditions whereas hydrophobic interactions of the substances with non-polar parts of the adsorbents were assumed to be predominant under reversed-phase conditions. PB - Research Inst Medicinal Plants, Budakalasz T2 - Journal of Planar Chromatography: Modern TLC / Thin Layer Chromatography T1 - The retention behaviour of some cholic acid derivatives on different adsorbents VL - 15 IS - 4 SP - 299 EP - 305 DO - 10.1556/JPC.15.2002.4.11 ER -
@article{ author = "Gaica, Sandra and Opsenica, Dejan and Šolaja, Bogdan and Tešić, Živoslav and Milojković-Opsenica, Dušanka", year = "2002", abstract = "The thin-layer chromatographic behavior of cholic acid and fourteen of its synthetic derivatives has been studied in six reversed-phase and three normal-phase systems. Four significantly different adsorbents were used - RP-18-silica, CN-silica, polyacrylonitrile adsorbent, and unmodified silica gel. Reversed-phase chromatography was performed with water-organic modifier (methanol, dioxane, or acetone) binary mobile phases of widely variable composition. An approximately linear relationship was obtained between R-M values and the amount of organic modifier in the mobile phases investigated. The effect of mobile-phase composition on retention has been considered and the selectivity of the chromatographic systems is also discussed. Separation mechanisms are proposed on the basis of the results obtained. A positive solvation effect was proposed as a predominant factor determining retention under normal-phase conditions whereas hydrophobic interactions of the substances with non-polar parts of the adsorbents were assumed to be predominant under reversed-phase conditions.", publisher = "Research Inst Medicinal Plants, Budakalasz", journal = "Journal of Planar Chromatography: Modern TLC / Thin Layer Chromatography", title = "The retention behaviour of some cholic acid derivatives on different adsorbents", volume = "15", number = "4", pages = "299-305", doi = "10.1556/JPC.15.2002.4.11" }
Gaica, S., Opsenica, D., Šolaja, B., Tešić, Ž.,& Milojković-Opsenica, D.. (2002). The retention behaviour of some cholic acid derivatives on different adsorbents. in Journal of Planar Chromatography: Modern TLC / Thin Layer Chromatography Research Inst Medicinal Plants, Budakalasz., 15(4), 299-305. https://doi.org/10.1556/JPC.15.2002.4.11
Gaica S, Opsenica D, Šolaja B, Tešić Ž, Milojković-Opsenica D. The retention behaviour of some cholic acid derivatives on different adsorbents. in Journal of Planar Chromatography: Modern TLC / Thin Layer Chromatography. 2002;15(4):299-305. doi:10.1556/JPC.15.2002.4.11 .
Gaica, Sandra, Opsenica, Dejan, Šolaja, Bogdan, Tešić, Živoslav, Milojković-Opsenica, Dušanka, "The retention behaviour of some cholic acid derivatives on different adsorbents" in Journal of Planar Chromatography: Modern TLC / Thin Layer Chromatography, 15, no. 4 (2002):299-305, https://doi.org/10.1556/JPC.15.2002.4.11 . .