CER - Central Repository
Institute of Chemistry, Technology and Metallurgy
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrilic)
    • Serbian (Latin)
  • Login
View Item 
  •   Central Repository
  • IHTM
  • Radovi istraživača / Researchers' publications
  • View Item
  •   Central Repository
  • IHTM
  • Radovi istraživača / Researchers' publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species

Thumbnail
2010
1934578x1000500511.pdf (287.3Kb)
Authors
Aljančić, Ivana
Stanković, Miroslava
Tešević, Vele
Vujisić, Ljubodrag V.
Vajs, Vlatka
Milosavljević, Slobodan
Article (Published version)
Metadata
Show full item record
Abstract
This study was conducted to elucidate the in vitro protective effect of five flavonoids [apigenin (1), apigenin-7-O-glucoside (2), centaureidin (3), jaceidin (4) and quercetin (5)] against chromosomal damage in mitogen-induced human lymphocytes. Using the Cytochalasin-B blocked micronucleus (CBMN) assay, in which the biomarker of chromosome breakage and/or chromosome loss is the elevated frequency of micronucleus (MN) in binucleated (BN) cells, the presence of flavonoid 2 in minimal concentration (3 mu g/mL) gave a 35.5% decrease M the frequency of MN when compared with control human lymphocytes. The same concentration of flavonoids 1, 3 and 4, reduced the MN frequency by 24.4%, 28.0% and 28.0%, respectively. Higher concentrations (6 mu g/mL and 10 mu g/mL) seemed less effective. Flavonoid 5 (3 mu g/mL.) induced a slight decrease in MN frequency (5%), while higher doses (6 mu g/mL and 10 mu g/mL) provoked an increase of DNA damage. The comparable values for the cytokinesis-block prolif...eration index (CBPI) of the tested flavonoids and positive control suggested an inhibitory effect on lymphocyte proliferation. In the DPPH scavenging assay, flavonoids 1-4 demonstrated modest activity, in a dose-dependent manner, compared with the synthetic antioxidants BHT and Trolox, while 5 exhibited comparably high antioxidative activity.

Keywords:
Flavonoids / CBMN / DPPH / BHT
Source:
Natural Product Communications, 2010, 5, 5, 729-732
Publisher:
  • Natural Products Inc, Westerville
Projects:
  • Sekundarni metaboliti samoniklih, lekovitih biljaka: izolovanje, karakterizacija i biloška aktivnost (RS-142053)

DOI: 10.1177/1934578X1000500511

ISSN: 1934-578X

PubMed: 20521537

WoS: 000277750200012

Scopus: 2-s2.0-77954115563
[ Google Scholar ]
11
9
URI
http://cer.ihtm.bg.ac.rs/handle/123456789/2738
Collections
  • Radovi istraživača / Researchers' publications
Institution
IHTM

DSpace software copyright © 2002-2015  DuraSpace
About CeR – Central Repository | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceInstitutionsAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About CeR – Central Repository | Send Feedback

OpenAIRERCUB