Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species
Authors
Aljančić, IvanaStanković, Miroslava

Tešević, Vele

Vujisić, Ljubodrag V.

Vajs, Vlatka
Milosavljević, Slobodan

Article (Published version)
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This study was conducted to elucidate the in vitro protective effect of five flavonoids [apigenin (1), apigenin-7-O-glucoside (2), centaureidin (3), jaceidin (4) and quercetin (5)] against chromosomal damage in mitogen-induced human lymphocytes. Using the Cytochalasin-B blocked micronucleus (CBMN) assay, in which the biomarker of chromosome breakage and/or chromosome loss is the elevated frequency of micronucleus (MN) in binucleated (BN) cells, the presence of flavonoid 2 in minimal concentration (3 mu g/mL) gave a 35.5% decrease M the frequency of MN when compared with control human lymphocytes. The same concentration of flavonoids 1, 3 and 4, reduced the MN frequency by 24.4%, 28.0% and 28.0%, respectively. Higher concentrations (6 mu g/mL and 10 mu g/mL) seemed less effective. Flavonoid 5 (3 mu g/mL.) induced a slight decrease in MN frequency (5%), while higher doses (6 mu g/mL and 10 mu g/mL) provoked an increase of DNA damage. The comparable values for the cytokinesis-block prolif...eration index (CBPI) of the tested flavonoids and positive control suggested an inhibitory effect on lymphocyte proliferation. In the DPPH scavenging assay, flavonoids 1-4 demonstrated modest activity, in a dose-dependent manner, compared with the synthetic antioxidants BHT and Trolox, while 5 exhibited comparably high antioxidative activity.
Keywords:
Flavonoids / CBMN / DPPH / BHTSource:
Natural Product Communications, 2010, 5, 5, 729-732Publisher:
- Natural Products Inc, Westerville
Funding / projects:
- Sekundarni metaboliti samoniklih, lekovitih biljaka: izolovanje, karakterizacija i biloška aktivnost (RS-142053)
DOI: 10.1177/1934578X1000500511
ISSN: 1934-578X
PubMed: 20521537
WoS: 000277750200012
Scopus: 2-s2.0-77954115563
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IHTMTY - JOUR AU - Aljančić, Ivana AU - Stanković, Miroslava AU - Tešević, Vele AU - Vujisić, Ljubodrag V. AU - Vajs, Vlatka AU - Milosavljević, Slobodan PY - 2010 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/2738 AB - This study was conducted to elucidate the in vitro protective effect of five flavonoids [apigenin (1), apigenin-7-O-glucoside (2), centaureidin (3), jaceidin (4) and quercetin (5)] against chromosomal damage in mitogen-induced human lymphocytes. Using the Cytochalasin-B blocked micronucleus (CBMN) assay, in which the biomarker of chromosome breakage and/or chromosome loss is the elevated frequency of micronucleus (MN) in binucleated (BN) cells, the presence of flavonoid 2 in minimal concentration (3 mu g/mL) gave a 35.5% decrease M the frequency of MN when compared with control human lymphocytes. The same concentration of flavonoids 1, 3 and 4, reduced the MN frequency by 24.4%, 28.0% and 28.0%, respectively. Higher concentrations (6 mu g/mL and 10 mu g/mL) seemed less effective. Flavonoid 5 (3 mu g/mL.) induced a slight decrease in MN frequency (5%), while higher doses (6 mu g/mL and 10 mu g/mL) provoked an increase of DNA damage. The comparable values for the cytokinesis-block proliferation index (CBPI) of the tested flavonoids and positive control suggested an inhibitory effect on lymphocyte proliferation. In the DPPH scavenging assay, flavonoids 1-4 demonstrated modest activity, in a dose-dependent manner, compared with the synthetic antioxidants BHT and Trolox, while 5 exhibited comparably high antioxidative activity. PB - Natural Products Inc, Westerville T2 - Natural Product Communications T1 - Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species VL - 5 IS - 5 SP - 729 EP - 732 DO - 10.1177/1934578X1000500511 ER -
@article{ author = "Aljančić, Ivana and Stanković, Miroslava and Tešević, Vele and Vujisić, Ljubodrag V. and Vajs, Vlatka and Milosavljević, Slobodan", year = "2010", abstract = "This study was conducted to elucidate the in vitro protective effect of five flavonoids [apigenin (1), apigenin-7-O-glucoside (2), centaureidin (3), jaceidin (4) and quercetin (5)] against chromosomal damage in mitogen-induced human lymphocytes. Using the Cytochalasin-B blocked micronucleus (CBMN) assay, in which the biomarker of chromosome breakage and/or chromosome loss is the elevated frequency of micronucleus (MN) in binucleated (BN) cells, the presence of flavonoid 2 in minimal concentration (3 mu g/mL) gave a 35.5% decrease M the frequency of MN when compared with control human lymphocytes. The same concentration of flavonoids 1, 3 and 4, reduced the MN frequency by 24.4%, 28.0% and 28.0%, respectively. Higher concentrations (6 mu g/mL and 10 mu g/mL) seemed less effective. Flavonoid 5 (3 mu g/mL.) induced a slight decrease in MN frequency (5%), while higher doses (6 mu g/mL and 10 mu g/mL) provoked an increase of DNA damage. The comparable values for the cytokinesis-block proliferation index (CBPI) of the tested flavonoids and positive control suggested an inhibitory effect on lymphocyte proliferation. In the DPPH scavenging assay, flavonoids 1-4 demonstrated modest activity, in a dose-dependent manner, compared with the synthetic antioxidants BHT and Trolox, while 5 exhibited comparably high antioxidative activity.", publisher = "Natural Products Inc, Westerville", journal = "Natural Product Communications", title = "Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species", volume = "5", number = "5", pages = "729-732", doi = "10.1177/1934578X1000500511" }
Aljančić, I., Stanković, M., Tešević, V., Vujisić, L. V., Vajs, V.,& Milosavljević, S.. (2010). Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species. in Natural Product Communications Natural Products Inc, Westerville., 5(5), 729-732. https://doi.org/10.1177/1934578X1000500511
Aljančić I, Stanković M, Tešević V, Vujisić LV, Vajs V, Milosavljević S. Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species. in Natural Product Communications. 2010;5(5):729-732. doi:10.1177/1934578X1000500511 .
Aljančić, Ivana, Stanković, Miroslava, Tešević, Vele, Vujisić, Ljubodrag V., Vajs, Vlatka, Milosavljević, Slobodan, "Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species" in Natural Product Communications, 5, no. 5 (2010):729-732, https://doi.org/10.1177/1934578X1000500511 . .