dc.creator | Ignjatović, Nenad | |
dc.creator | Penov Gaši, Katarina | |
dc.creator | Wu, Victoria | |
dc.creator | Ajduković, Jovana | |
dc.creator | Kojić, Vesna V. | |
dc.creator | Vasiljević-Radović, Dana | |
dc.creator | Kuzmanović, Maja | |
dc.creator | Uskoković, Vuk | |
dc.creator | Uskoković, Dragan P. | |
dc.date.accessioned | 2019-04-01T14:14:05Z | |
dc.date.available | 2018-09-28 | |
dc.date.available | 2019-04-01T14:14:05Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 0927-7765 (Print) | |
dc.identifier.issn | 1873-4367 (Online) | |
dc.identifier.uri | http://dais.sanu.ac.rs/123456789/15984 | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/2644 | |
dc.description.abstract | In an earlier study we demonstrated that hydroxyapatite nanoparticles coated with chitosan-poly(d,l)-lactide-co-glycolide (HAp/Ch-PLGA) target lungs following their intravenous injection into mice. In this study we utilize an emulsification process and freeze drying to load the composite HAp/Ch-PLGA particles with 17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (A), a chemotherapeutic derivative of androstane and a novel compound with a selective anticancer activity against lung cancer cells. 1H NMR and 13C NMR techniques confirmed the intact structure of the derivative A following its entrapment within HAp/Ch-PLGA particles. The thermogravimetric and differential thermal analyses coupled with mass spectrometry were used to assess the thermal degradation products and properties of A-loaded HAp/Ch-PLGA. The loading efficiency, as indicated by the comparison of enthalpies of phase transitions in pure A and A-loaded HAp/Ch-PLGA, equaled 7.47 wt.%. The release of A from HAp/Ch-PLGA was sustained, neither exhibiting a burst release nor plateauing after three weeks. Atomic force microscopy and particle size distribution analyses were used to confirm that the particles were spherical with a uniform size distribution of d50 = 168 nm. In vitro cytotoxicity testing of A-loaded HAp/Ch-PLGA using MTT and trypan blue dye exclusion assays demonstrated that the particles were cytotoxic to the A549 human lung carcinoma cell line (46 ± 2%), while simultaneously preserving high viability (83 ± 3%) of regular MRC5 human lung fibroblasts and causing no harm to primary mouse lung fibroblasts. In conclusion, composite A-loaded HAp/Ch-PLGA particles could be seen as promising drug delivery platforms for selective cancer therapies, targeting malignant cells for destruction, while having a significantly lesser cytotoxic effect on the healthy cells. | eng |
dc.language | en | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45004/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172021/RS// | |
dc.relation | United States National Institutes of Health (NIH), Grant R00-DE021416 | |
dc.rights | embargoedAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Colloids and Surfaces B: Biointerfaces | eng |
dc.subject | androstane | |
dc.subject | chitosan | |
dc.subject | hydroxyapatite | |
dc.subject | lung cancer | |
dc.subject | PLGA | |
dc.title | Selective anticancer activity of hydroxyapatite/chitosan-poly(D,L)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor | en |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dcterms.abstract | Игњатовић, Ненад Л.; Ускоковић, Драган П.; Пенов Гаши, Катарина; Ускоковић, Вук; Кузмановић, Маја; Васиљевић Радовић, Дана; Којић, Весна; Aјдуковић, Јована; Wу, Вицториа; | |
dc.citation.volume | 148 | |
dc.citation.spage | 629 | |
dc.citation.epage | 639 | |
dc.description.other | This is the peer-reviewed version of the articleIgnjatović, N.L., Penov-Gaši, K.M., Wu, V.M., Ajduković, J.J., Kojić, V.V., Vasiljević-Radović, D., Kuzmanović, M., Uskoković, V., Uskoković, D.P., 2016. Selective anticancer activity of hydroxyapatite/chitosan-poly(d,l)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor. Colloids and Surfaces B: Biointerfaces 148, 629–639. [https://doi.org/10.1016/j.colsurfb.2016.09.041] | |
dc.identifier.pmid | 27694053 | |
dc.identifier.doi | 10.1016/j.colsurfb.2016.09.041 | |
dc.identifier.fulltext | https://cer.ihtm.bg.ac.rs/bitstream/id/6236/10.1016-j.colsurfb.2016.09.041.pdf | |
dc.identifier.scopus | 2-s2.0-84989184184 | |
dc.identifier.wos | 000388248500073 | |
dc.type.version | acceptedVersion | |