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dc.creatorIgnjatović, Nenad
dc.creatorPenov Gaši, Katarina
dc.creatorWu, Victoria
dc.creatorAjduković, Jovana
dc.creatorKojić, Vesna V.
dc.creatorVasiljević-Radović, Dana
dc.creatorKuzmanović, Maja
dc.creatorUskoković, Vuk
dc.creatorUskoković, Dragan P.
dc.date.accessioned2019-04-01T14:14:05Z
dc.date.available2018-09-28
dc.date.available2019-04-01T14:14:05Z
dc.date.issued2016
dc.identifier.issn0927-7765 (Print)
dc.identifier.issn1873-4367 (Online)
dc.identifier.urihttp://dais.sanu.ac.rs/123456789/15984
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/2644
dc.description.abstractIn an earlier study we demonstrated that hydroxyapatite nanoparticles coated with chitosan-poly(d,l)-lactide-co-glycolide (HAp/Ch-PLGA) target lungs following their intravenous injection into mice. In this study we utilize an emulsification process and freeze drying to load the composite HAp/Ch-PLGA particles with 17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (A), a chemotherapeutic derivative of androstane and a novel compound with a selective anticancer activity against lung cancer cells. 1H NMR and 13C NMR techniques confirmed the intact structure of the derivative A following its entrapment within HAp/Ch-PLGA particles. The thermogravimetric and differential thermal analyses coupled with mass spectrometry were used to assess the thermal degradation products and properties of A-loaded HAp/Ch-PLGA. The loading efficiency, as indicated by the comparison of enthalpies of phase transitions in pure A and A-loaded HAp/Ch-PLGA, equaled 7.47 wt.%. The release of A from HAp/Ch-PLGA was sustained, neither exhibiting a burst release nor plateauing after three weeks. Atomic force microscopy and particle size distribution analyses were used to confirm that the particles were spherical with a uniform size distribution of d50 = 168 nm. In vitro cytotoxicity testing of A-loaded HAp/Ch-PLGA using MTT and trypan blue dye exclusion assays demonstrated that the particles were cytotoxic to the A549 human lung carcinoma cell line (46 ± 2%), while simultaneously preserving high viability (83 ± 3%) of regular MRC5 human lung fibroblasts and causing no harm to primary mouse lung fibroblasts. In conclusion, composite A-loaded HAp/Ch-PLGA particles could be seen as promising drug delivery platforms for selective cancer therapies, targeting malignant cells for destruction, while having a significantly lesser cytotoxic effect on the healthy cells.eng
dc.languageen
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45004/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172021/RS//
dc.relationUnited States National Institutes of Health (NIH), Grant R00-DE021416
dc.rightsembargoedAccess
dc.sourceColloids and Surfaces B: Biointerfaceseng
dc.subjectandrostane
dc.subjectchitosan
dc.subjecthydroxyapatite
dc.subjectlung cancer
dc.subjectPLGA
dc.titleSelective anticancer activity of hydroxyapatite/chitosan-poly(D,L)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitoren
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractИгњатовић, Ненад Л.; Ускоковић, Драган П.; Пенов Гаши, Катарина; Ускоковић, Вук; Кузмановић, Маја; Васиљевић Радовић, Дана; Којић, Весна; Aјдуковић, Јована; Wу, Вицториа;
dc.citation.volume148
dc.citation.spage629
dc.citation.epage639
dc.description.otherThis is the peer-reviewed version of the articleIgnjatović, N.L., Penov-Gaši, K.M., Wu, V.M., Ajduković, J.J., Kojić, V.V., Vasiljević-Radović, D., Kuzmanović, M., Uskoković, V., Uskoković, D.P., 2016. Selective anticancer activity of hydroxyapatite/chitosan-poly(d,l)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor. Colloids and Surfaces B: Biointerfaces 148, 629–639. [https://doi.org/10.1016/j.colsurfb.2016.09.041]
dc.identifier.pmid27694053
dc.identifier.doi10.1016/j.colsurfb.2016.09.041
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs/bitstream/id/6236/10.1016-j.colsurfb.2016.09.041.pdf
dc.identifier.scopus2-s2.0-84989184184
dc.identifier.wos000388248500073
dc.type.versionacceptedVersion


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