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dc.creatorGojgić-Cvijović, Gordana
dc.creatorJakovljević, Dragica
dc.creatorLončarević, Branka
dc.creatorTodorović, Nina
dc.creatorPergal, Marija
dc.creatorCiric, J.
dc.creatorLoos, K.
dc.creatorBeškoski, Vladimir
dc.creatorVrvić, Miroslav
dc.date.accessioned2019-01-30T18:01:56Z
dc.date.available2019-10-03
dc.date.issued2019
dc.identifier.issn0141-8130
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/2503
dc.description.abstractThe production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100-140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 532 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 x 10(6) Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium with 200 g/L sucrose.en
dc.publisherAmsterdam : Elsevier Science Bv, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43004/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172053/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172062/RS//
dc.rightsembargoedAccess
dc.sourceInternational Journal of Biological Macromolecules
dc.subjectLevanen
dc.subjectMolassesen
dc.subjectMolecular weighten
dc.titleProduction of levan by Bacillus licheniformis NS032 in sugar beet molasses-based mediumen
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractВрвиц, М. М.; Цириц, Ј.; Бескоски, Владимир; Лоос, К.; Пергал, Марија; Лончаревић, Бранка; Јаковљевић, Драгица; Гојгић Цвијовић, Гордана; Тодоровић, Нина;
dc.citation.volume121
dc.citation.spage142
dc.citation.epage151
dc.citation.other121: 142-151
dc.citation.rankaM21~
dc.description.otherThis is peer reviewed version of the following article: Gojgic-Cvijovic, G. D.; Jakovljevic, D. M.; Loncarevic, B. D.; Todorovic, N. M.; Pergal, M. V.; Ciric, J.; Loos, K.; Beškoski, V.; Vrvić, M. M. Production of Levan by Bacillus Licheniformis NS032 in Sugar Beet Molasses-Based Medium. International Journal of Biological Macromolecules 2019, 121, 142–151. [https://doi.org/10.1016/j.ijbiomac.2018.10.019]
dc.description.other[http://cer.ihtm.bg.ac.rs/handle/123456789/2926]
dc.identifier.pmid30291934
dc.identifier.doi10.1016/j.ijbiomac.2018.10.019
dc.identifier.rcubConv_4032
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs/bitstream/id/8860/2501.pdf
dc.identifier.scopus2-s2.0-85054437248
dc.identifier.wos000452346100018
dc.type.versionacceptedVersion


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