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Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium

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2019
2501.pdf (3.484Mb)
Authors
Gojgić-Cvijović, Gordana
Jakovljević, Dragica
Lončarević, Branka
Todorović, Nina
Pergal, Marija
Ciric, J.
Loos, K.
Beškoski, Vladimir
Vrvić, Miroslav
Article (Accepted Version)
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Abstract
The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100-140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 532 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 x 10(6) Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium... with 200 g/L sucrose.

Keywords:
Levan / Molasses / Molecular weight
Source:
International Journal of Biological Macromolecules, 2019, 121, 142-151
Publisher:
  • Amsterdam : Elsevier Science Bv, Amsterdam
Projects:
  • Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-43004)
  • Natural products of wild, cultivated and edible plants: structure and bioactivity determination (RS-172053)
  • Synthesis and characterization of novel functional polymers and polymeric nanocomposites (RS-172062)
Note:
  • This is peer reviewed version of the following article: Gojgic-Cvijovic, G. D.; Jakovljevic, D. M.; Loncarevic, B. D.; Todorovic, N. M.; Pergal, M. V.; Ciric, J.; Loos, K.; Beškoski, V.; Vrvić, M. M. Production of Levan by Bacillus Licheniformis NS032 in Sugar Beet Molasses-Based Medium. International Journal of Biological Macromolecules 2019, 121, 142–151. https://doi.org/10.1016/j.ijbiomac.2018.10.019
  • http://cer.ihtm.bg.ac.rs/handle/123456789/2926

DOI: 10.1016/j.ijbiomac.2018.10.019

ISSN: 0141-8130

PubMed: 30291934

WoS: 000452346100018

Scopus: 2-s2.0-85054437248
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URI
http://cer.ihtm.bg.ac.rs/handle/123456789/2503
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