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dc.creatorJovanovic, Jovana
dc.creatorKolarevic, Stoimir
dc.creatorMiloskovic, Aleksandra
dc.creatorRadojkovic, Natasa
dc.creatorSimić, Vladica
dc.creatorDojčinović, Biljana
dc.creatorKracun-Kolarevic, Margareta
dc.creatorPaunović, Momir
dc.creatorKostic, Jovana
dc.creatorSunjog, Karolina
dc.creatorTimilijic, Jovana
dc.creatorĐorđević, Jelena B.
dc.creatorGačić, Zoran
dc.creatorZegura, Bojana
dc.creatorVukovic-Gacic, Branka
dc.date.accessioned2019-01-30T18:01:08Z
dc.date.available2019-01-30T18:01:08Z
dc.date.issued2018
dc.identifier.issn0048-9697
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/2468
dc.description.abstractThe Velika Morava River is the greatest national Serbian river and the significant tributary of the Danube River. The major problems in the Velika Morava River Basin (VMRB) represent untreated industrial and municipal wastewaters. In this study, the level of genotoxic potential at the sites along the VMRB was evaluated by parallel in vitro and in situ approach. Within in vitro testing, genotoxicity of native water samples collected from the sites in VMRB was evaluated by SOS/umuC test on Salmonella typhimurium TA1535/pSK1002 and by the comet assay on HepG2 cells. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet (alkaline and Fpg-modified comet) and micronucleus assays. Additionally, the concentration of heavy metals in fish tissue was measured and this data, compiled with the data of the physico-chemical parameters measured in water, was used as a measure of the pollution pressure at the sites. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests and should be taken with precaution when making predictions on the status of the ecosystem. Within applied battery of in situ assays differential sensitivity of assays was observed where alkaline comet assay showed the highest potential in differentiation of the sites based on genotoxic potential. Integrated biomarker response showed that usage of the battery of bioassays provides better insight in a genotoxic effects in animals, and consequently, that the holistic approach is more suitable for this type of study.en
dc.publisherElsevier Science Bv, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31011/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/37009/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173045/RS//
dc.rightsrestrictedAccess
dc.sourceScience of the Total Environment
dc.subjectEco/genotoxicityen
dc.subjectSOS/umuCen
dc.subjectHepG2 cellsen
dc.subjectComet assayen
dc.subjectThe Velika Morava Riveren
dc.subjectAlburnus alburnusen
dc.titleEvaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractПауновић, Момир; Гациц, Зоран; Дјордјевиц, Јелена; Симиц, Владица; Милосковиц, Aлександра; Дојчиновић, Биљана; Јовановиц, Јована; Тимилијиц, Јована; Зегура, Бојана; Вуковиц-Гациц, Бранка; Суњог, Каролина; Коларевиц, Стоимир; Крацун-Коларевиц, Маргарета; Костиц, Јована; Радојковиц, Натаса;
dc.citation.volume621
dc.citation.spage1289
dc.citation.epage1299
dc.citation.other621: 1289-1299
dc.citation.rankaM21
dc.identifier.pmid29056382
dc.identifier.doi10.1016/j.scitotenv.2017.10.099
dc.identifier.rcubConv_3867
dc.identifier.scopus2-s2.0-85031791196
dc.identifier.wos000424196800127
dc.type.versionpublishedVersion


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