Herein, we propose a H-1 NMR-based metabolomics method to reveal cytotoxic metabolites from Mahonia aquifolium stem-bark. Primary and secondary metabolites in the Mahonia aquifolium extracts were identified by thorough analysis of H-1 and 2D NMR spectra, without prior isolation. An OPLS multivariate analysis method was used to correlate the chemical composition of the plant extracts with the results of cytotoxic activity against Human cervical adenocarcinoma cell line. Protoberberine alkaloids berberine and palmatine, along with bisbenzylisoquinoline alkaloid berbamine were identified as the most influential in the OPLS model, with the highest cytotoxic activity.
TY - JOUR
AU - Gođevac, Dejan
AU - Damjanovic, Ana
AU - Stanojković, Tatjana
AU - Anđelković, Boban D.
AU - Zdunić, Gordana
PY - 2018
UR - https://cer.ihtm.bg.ac.rs/handle/123456789/2464
AB - Herein, we propose a H-1 NMR-based metabolomics method to reveal cytotoxic metabolites from Mahonia aquifolium stem-bark. Primary and secondary metabolites in the Mahonia aquifolium extracts were identified by thorough analysis of H-1 and 2D NMR spectra, without prior isolation. An OPLS multivariate analysis method was used to correlate the chemical composition of the plant extracts with the results of cytotoxic activity against Human cervical adenocarcinoma cell line. Protoberberine alkaloids berberine and palmatine, along with bisbenzylisoquinoline alkaloid berbamine were identified as the most influential in the OPLS model, with the highest cytotoxic activity.
PB - Elsevier
T2 - Journal of Pharmaceutical and Biomedical Analysis
T1 - Identification of cytotoxic metabolites from Mahonia aquifolium using H-1 NMR-based metabolomics approach
VL - 150
SP - 9
EP - 14
DO - 10.1016/j.jpba.2017.11.075
ER -
@article{
author = "Gođevac, Dejan and Damjanovic, Ana and Stanojković, Tatjana and Anđelković, Boban D. and Zdunić, Gordana",
year = "2018",
abstract = "Herein, we propose a H-1 NMR-based metabolomics method to reveal cytotoxic metabolites from Mahonia aquifolium stem-bark. Primary and secondary metabolites in the Mahonia aquifolium extracts were identified by thorough analysis of H-1 and 2D NMR spectra, without prior isolation. An OPLS multivariate analysis method was used to correlate the chemical composition of the plant extracts with the results of cytotoxic activity against Human cervical adenocarcinoma cell line. Protoberberine alkaloids berberine and palmatine, along with bisbenzylisoquinoline alkaloid berbamine were identified as the most influential in the OPLS model, with the highest cytotoxic activity.",
publisher = "Elsevier",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Identification of cytotoxic metabolites from Mahonia aquifolium using H-1 NMR-based metabolomics approach",
volume = "150",
pages = "9-14",
doi = "10.1016/j.jpba.2017.11.075"
}
Gođevac, D., Damjanovic, A., Stanojković, T., Anđelković, B. D.,& Zdunić, G.. (2018). Identification of cytotoxic metabolites from Mahonia aquifolium using H-1 NMR-based metabolomics approach. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier., 150, 9-14.
https://doi.org/10.1016/j.jpba.2017.11.075
Gođevac D, Damjanovic A, Stanojković T, Anđelković BD, Zdunić G. Identification of cytotoxic metabolites from Mahonia aquifolium using H-1 NMR-based metabolomics approach. in Journal of Pharmaceutical and Biomedical Analysis. 2018;150:9-14.
doi:10.1016/j.jpba.2017.11.075 .
Gođevac, Dejan, Damjanovic, Ana, Stanojković, Tatjana, Anđelković, Boban D., Zdunić, Gordana, "Identification of cytotoxic metabolites from Mahonia aquifolium using H-1 NMR-based metabolomics approach" in Journal of Pharmaceutical and Biomedical Analysis, 150 (2018):9-14,
https://doi.org/10.1016/j.jpba.2017.11.075 . .
