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Interaction of Au(iii) and Pt(ii) complexes with Na/K-ATPase: experimental and theoretical study of reaction stoichiometry and binding sites

Authorized Users Only
2018
Authors
Vujačić Nikezić, Ana V.
Janjić, Goran
Bondžić, Aleksandra M.
Zarić, Božidarka
Vasic-Anicijevic, Dragana D.
Momic, Tatjana G.
Vasić, Vesna M.
Article (Published version)
Metadata
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Abstract
The present paper deals with investigation of the interaction between selected simple structure Au(iii) ([AuCl4](-), [AuCl2(dmso)(2)](+), [AuCl2(bipy)](+)) and Pt(ii) ([PtCl2(dmso)(2)]) complexes with Na/K-ATPase as the target enzyme, using an experimental and theoretical approach. Reaction stoichiometries and binding constants for these enzyme/complex systems were determined, while kinetic measurements were used in order to reveal the type of inhibition. Based on the results obtained by quantum mechanical calculations (electrostatic surface potential (ESP), volume and surface of the complexes) the nature of the investigated complexes was characterized. By using the solvent accessible surface area (SASA) applied on specific inhibitory sites (ion channel and intracellular domains) the nature of these sites was described. Docking studies were used to determine the theoretical probability of the non-covalent metal binding site positions. Inhibition studies implied that all the investigate...d complexes decreased the activity of the enzyme while the kinetic analysis indicated an uncompetitive mode of inhibition for the selected complexes. Docking results suggested that the main inhibitory site of all these complexes is located in the ion translocation pathway on the extracellular side in the E2P enzyme conformation, similar to the case of cardiac glycosides, specific Na/K-ATPase inhibitors. Also, based on our knowledge, the hydrolyzed forms of [AuCl4](-) and [PtCl2(dmso)(2)] complexes were investigated for the first time by theoretical calculations in this paper. Thereby, a new inhibitory site situated between the M2 and M4 helices was revealed. Binding in this site induces conformational changes in the enzyme domains and perturbs the E1-E2P conformational equilibrium, causing enzyme inhibition.

Source:
Metallomics, 2018, 10, 7, 1003-1015
Publisher:
  • Royal Soc Chemistry, Cambridge
Funding / projects:
  • Studies of enzyme interactions with toxic and pharmacologically active molecules (RS-172023)
  • Ministry of Education, Science and Technological Development of the Republic of Serbia

DOI: 10.1039/c8mt00111a

ISSN: 1756-5901

PubMed: 29978878

WoS: 000439583800011

Scopus: 2-s2.0-85050547953
[ Google Scholar ]
3
3
URI
https://cer.ihtm.bg.ac.rs/handle/123456789/2452
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - JOUR
AU  - Vujačić Nikezić, Ana V.
AU  - Janjić, Goran
AU  - Bondžić, Aleksandra M.
AU  - Zarić, Božidarka
AU  - Vasic-Anicijevic, Dragana D.
AU  - Momic, Tatjana G.
AU  - Vasić, Vesna M.
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2452
AB  - The present paper deals with investigation of the interaction between selected simple structure Au(iii) ([AuCl4](-), [AuCl2(dmso)(2)](+), [AuCl2(bipy)](+)) and Pt(ii) ([PtCl2(dmso)(2)]) complexes with Na/K-ATPase as the target enzyme, using an experimental and theoretical approach. Reaction stoichiometries and binding constants for these enzyme/complex systems were determined, while kinetic measurements were used in order to reveal the type of inhibition. Based on the results obtained by quantum mechanical calculations (electrostatic surface potential (ESP), volume and surface of the complexes) the nature of the investigated complexes was characterized. By using the solvent accessible surface area (SASA) applied on specific inhibitory sites (ion channel and intracellular domains) the nature of these sites was described. Docking studies were used to determine the theoretical probability of the non-covalent metal binding site positions. Inhibition studies implied that all the investigated complexes decreased the activity of the enzyme while the kinetic analysis indicated an uncompetitive mode of inhibition for the selected complexes. Docking results suggested that the main inhibitory site of all these complexes is located in the ion translocation pathway on the extracellular side in the E2P enzyme conformation, similar to the case of cardiac glycosides, specific Na/K-ATPase inhibitors. Also, based on our knowledge, the hydrolyzed forms of [AuCl4](-) and [PtCl2(dmso)(2)] complexes were investigated for the first time by theoretical calculations in this paper. Thereby, a new inhibitory site situated between the M2 and M4 helices was revealed. Binding in this site induces conformational changes in the enzyme domains and perturbs the E1-E2P conformational equilibrium, causing enzyme inhibition.
PB  - Royal Soc Chemistry, Cambridge
T2  - Metallomics
T1  - Interaction of Au(iii) and Pt(ii) complexes with Na/K-ATPase: experimental and theoretical study of reaction stoichiometry and binding sites
VL  - 10
IS  - 7
SP  - 1003
EP  - 1015
DO  - 10.1039/c8mt00111a
ER  - 
@article{
author = "Vujačić Nikezić, Ana V. and Janjić, Goran and Bondžić, Aleksandra M. and Zarić, Božidarka and Vasic-Anicijevic, Dragana D. and Momic, Tatjana G. and Vasić, Vesna M.",
year = "2018",
abstract = "The present paper deals with investigation of the interaction between selected simple structure Au(iii) ([AuCl4](-), [AuCl2(dmso)(2)](+), [AuCl2(bipy)](+)) and Pt(ii) ([PtCl2(dmso)(2)]) complexes with Na/K-ATPase as the target enzyme, using an experimental and theoretical approach. Reaction stoichiometries and binding constants for these enzyme/complex systems were determined, while kinetic measurements were used in order to reveal the type of inhibition. Based on the results obtained by quantum mechanical calculations (electrostatic surface potential (ESP), volume and surface of the complexes) the nature of the investigated complexes was characterized. By using the solvent accessible surface area (SASA) applied on specific inhibitory sites (ion channel and intracellular domains) the nature of these sites was described. Docking studies were used to determine the theoretical probability of the non-covalent metal binding site positions. Inhibition studies implied that all the investigated complexes decreased the activity of the enzyme while the kinetic analysis indicated an uncompetitive mode of inhibition for the selected complexes. Docking results suggested that the main inhibitory site of all these complexes is located in the ion translocation pathway on the extracellular side in the E2P enzyme conformation, similar to the case of cardiac glycosides, specific Na/K-ATPase inhibitors. Also, based on our knowledge, the hydrolyzed forms of [AuCl4](-) and [PtCl2(dmso)(2)] complexes were investigated for the first time by theoretical calculations in this paper. Thereby, a new inhibitory site situated between the M2 and M4 helices was revealed. Binding in this site induces conformational changes in the enzyme domains and perturbs the E1-E2P conformational equilibrium, causing enzyme inhibition.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Metallomics",
title = "Interaction of Au(iii) and Pt(ii) complexes with Na/K-ATPase: experimental and theoretical study of reaction stoichiometry and binding sites",
volume = "10",
number = "7",
pages = "1003-1015",
doi = "10.1039/c8mt00111a"
}
Vujačić Nikezić, A. V., Janjić, G., Bondžić, A. M., Zarić, B., Vasic-Anicijevic, D. D., Momic, T. G.,& Vasić, V. M.. (2018). Interaction of Au(iii) and Pt(ii) complexes with Na/K-ATPase: experimental and theoretical study of reaction stoichiometry and binding sites. in Metallomics
Royal Soc Chemistry, Cambridge., 10(7), 1003-1015.
https://doi.org/10.1039/c8mt00111a
Vujačić Nikezić AV, Janjić G, Bondžić AM, Zarić B, Vasic-Anicijevic DD, Momic TG, Vasić VM. Interaction of Au(iii) and Pt(ii) complexes with Na/K-ATPase: experimental and theoretical study of reaction stoichiometry and binding sites. in Metallomics. 2018;10(7):1003-1015.
doi:10.1039/c8mt00111a .
Vujačić Nikezić, Ana V., Janjić, Goran, Bondžić, Aleksandra M., Zarić, Božidarka, Vasic-Anicijevic, Dragana D., Momic, Tatjana G., Vasić, Vesna M., "Interaction of Au(iii) and Pt(ii) complexes with Na/K-ATPase: experimental and theoretical study of reaction stoichiometry and binding sites" in Metallomics, 10, no. 7 (2018):1003-1015,
https://doi.org/10.1039/c8mt00111a . .

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