Evaluation of genotoxic potential of tert-butylquinone and its derivatives in prokaryotic and eukaryotic test models
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2018
Authors
Đorđević, J.Kolarević, S.
Jovanović, J.
Kostić-Vuković, J.
Novaković, Irena

Jeremić, M.
Sladić, Dušan

Vuković-Gačić, Branka

Article (Published version)

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Tert-butylquinone (TBQ) and its alkylamino and aralkylamino derivatives are of high interest as a potential antitumor agent. Therefore, it was necessary to investigate if the compounds exert undesirable activities such as interaction with DNA molecule which could result in negative side effects in the case of their use in the diseases treatment. The major aim of this study was to investigate genotoxic potential of TBQ and selected derivatives in an acellular model by using plasmid DNA, in the prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002 and in eukaryotic models by using comet assay in human fetal lung cell line (MRC-5) and human liver cancer cell line (HepG2). Results indicated that in the acellular model TBQ and its derivatives do not interact with plasmid pUC19. In the prokaryotic model, only TBQ exerted weak genotoxic potential and only at highly cytotoxic concentrations. In eukaryotic models, genotoxic potential was detected mainly at the highest... concentrations of the tested substances but the effect was lower in both cell lines in comparison with benzo[a]pyrene and etoposide which were used as positive controls. Weak genotoxic potential of tested compounds recommends them as good candidates for further testing in development of new antitumor agents.
Keywords:
alkylamino and aralkylamino derivatives / comet assay / genotoxicity / plasmid relaxation / SOS/umuC / Tert-butylquinoneSource:
Drug and Chemical Toxicology, 2018, 522-530Publisher:
- Taylor and Francis Ltd
Funding / projects:
- Interactions of natural products, their derivatives and coordination compounds with proteins and nucleic acids (RS-172055)
- Bioactive natural products as potential sources of new pharmaceuticals and food supplements (RS-172058)
DOI: 10.1080/01480545.2018.1514043
ISSN: 0148-0545
WoS: 000551592600008
Scopus: 2-s2.0-85053908360
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IHTMTY - JOUR AU - Đorđević, J. AU - Kolarević, S. AU - Jovanović, J. AU - Kostić-Vuković, J. AU - Novaković, Irena AU - Jeremić, M. AU - Sladić, Dušan AU - Vuković-Gačić, Branka PY - 2018 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/2415 AB - Tert-butylquinone (TBQ) and its alkylamino and aralkylamino derivatives are of high interest as a potential antitumor agent. Therefore, it was necessary to investigate if the compounds exert undesirable activities such as interaction with DNA molecule which could result in negative side effects in the case of their use in the diseases treatment. The major aim of this study was to investigate genotoxic potential of TBQ and selected derivatives in an acellular model by using plasmid DNA, in the prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002 and in eukaryotic models by using comet assay in human fetal lung cell line (MRC-5) and human liver cancer cell line (HepG2). Results indicated that in the acellular model TBQ and its derivatives do not interact with plasmid pUC19. In the prokaryotic model, only TBQ exerted weak genotoxic potential and only at highly cytotoxic concentrations. In eukaryotic models, genotoxic potential was detected mainly at the highest concentrations of the tested substances but the effect was lower in both cell lines in comparison with benzo[a]pyrene and etoposide which were used as positive controls. Weak genotoxic potential of tested compounds recommends them as good candidates for further testing in development of new antitumor agents. PB - Taylor and Francis Ltd T2 - Drug and Chemical Toxicology T1 - Evaluation of genotoxic potential of tert-butylquinone and its derivatives in prokaryotic and eukaryotic test models SP - 522 EP - 530 DO - 10.1080/01480545.2018.1514043 ER -
@article{ author = "Đorđević, J. and Kolarević, S. and Jovanović, J. and Kostić-Vuković, J. and Novaković, Irena and Jeremić, M. and Sladić, Dušan and Vuković-Gačić, Branka", year = "2018", abstract = "Tert-butylquinone (TBQ) and its alkylamino and aralkylamino derivatives are of high interest as a potential antitumor agent. Therefore, it was necessary to investigate if the compounds exert undesirable activities such as interaction with DNA molecule which could result in negative side effects in the case of their use in the diseases treatment. The major aim of this study was to investigate genotoxic potential of TBQ and selected derivatives in an acellular model by using plasmid DNA, in the prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002 and in eukaryotic models by using comet assay in human fetal lung cell line (MRC-5) and human liver cancer cell line (HepG2). Results indicated that in the acellular model TBQ and its derivatives do not interact with plasmid pUC19. In the prokaryotic model, only TBQ exerted weak genotoxic potential and only at highly cytotoxic concentrations. In eukaryotic models, genotoxic potential was detected mainly at the highest concentrations of the tested substances but the effect was lower in both cell lines in comparison with benzo[a]pyrene and etoposide which were used as positive controls. Weak genotoxic potential of tested compounds recommends them as good candidates for further testing in development of new antitumor agents.", publisher = "Taylor and Francis Ltd", journal = "Drug and Chemical Toxicology", title = "Evaluation of genotoxic potential of tert-butylquinone and its derivatives in prokaryotic and eukaryotic test models", pages = "522-530", doi = "10.1080/01480545.2018.1514043" }
Đorđević, J., Kolarević, S., Jovanović, J., Kostić-Vuković, J., Novaković, I., Jeremić, M., Sladić, D.,& Vuković-Gačić, B.. (2018). Evaluation of genotoxic potential of tert-butylquinone and its derivatives in prokaryotic and eukaryotic test models. in Drug and Chemical Toxicology Taylor and Francis Ltd., 522-530. https://doi.org/10.1080/01480545.2018.1514043
Đorđević J, Kolarević S, Jovanović J, Kostić-Vuković J, Novaković I, Jeremić M, Sladić D, Vuković-Gačić B. Evaluation of genotoxic potential of tert-butylquinone and its derivatives in prokaryotic and eukaryotic test models. in Drug and Chemical Toxicology. 2018;:522-530. doi:10.1080/01480545.2018.1514043 .
Đorđević, J., Kolarević, S., Jovanović, J., Kostić-Vuković, J., Novaković, Irena, Jeremić, M., Sladić, Dušan, Vuković-Gačić, Branka, "Evaluation of genotoxic potential of tert-butylquinone and its derivatives in prokaryotic and eukaryotic test models" in Drug and Chemical Toxicology (2018):522-530, https://doi.org/10.1080/01480545.2018.1514043 . .