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Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode

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2018
131212340.pdf (737.0Kb)
Authors
Lović, Jelena
Stevanović, Sanja
Anđelković, Boban D.
Petrovic, S.
Vukovic, D.
Prlainović, Nevena Ž.
Mijin, Dušan
Nikolić, Nebojša D.
Avramov Ivić, Milka
Article (Published version)
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Abstract
Glucose biosensor containing cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is constructed and already electrochemically tested. Now, the electrochemical behavior of biosensor in human serum is further investigated and supported by morphological characterization of layers for the first time. The morphology and microstructure of layers was examined by Fourier transformed infra red spectroscopy (FTIR), atomic force (AFM) and optical microscopy (OM). The electrochemical indication that the Cys-GA-GOx film on Au surface is not compact and that there were some bare regions which remain catalytically active is supported by AFM and OM results. The construction and the nature of bonding of Au-Cys-GA-GOx biosensor layers is confirmed by the FTIR study.
Keywords:
Biosensor / Electrochemical glucose sensing / Microstructural characterization / FTIR / AFM / OM microstructural characterization
Source:
International Journal of Electrochemical Science, 2018, 13, 12, 12340-12348
Publisher:
  • Esg, Belgrade
Funding / projects:
  • Study of the Synthesis, Structure and Activity of Natural and Synthetic Organic Compounds (RS-172013)
  • New approach in designing materials for energy conversion and energy storage systems (RS-172060)

DOI: 10.20964/2018.12.59

ISSN: 1452-3981

WoS: 000452744500080

Scopus: 2-s2.0-85058157122
[ Google Scholar ]
2
1
URI
https://cer.ihtm.bg.ac.rs/handle/123456789/2364
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - JOUR
AU  - Lović, Jelena
AU  - Stevanović, Sanja
AU  - Anđelković, Boban D.
AU  - Petrovic, S.
AU  - Vukovic, D.
AU  - Prlainović, Nevena Ž.
AU  - Mijin, Dušan
AU  - Nikolić, Nebojša D.
AU  - Avramov Ivić, Milka
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2364
AB  - Glucose biosensor containing cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is constructed and already electrochemically tested. Now, the electrochemical behavior of biosensor in human serum is further investigated and supported by morphological characterization of layers for the first time. The morphology and microstructure of layers was examined by Fourier transformed infra red spectroscopy (FTIR), atomic force (AFM) and optical microscopy (OM). The electrochemical indication that the Cys-GA-GOx film on Au surface is not compact and that there were some bare regions which remain catalytically active is supported by AFM and OM results. The construction and the nature of bonding of Au-Cys-GA-GOx biosensor layers is confirmed by the FTIR study.
PB  - Esg, Belgrade
T2  - International Journal of Electrochemical Science
T1  - Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode
VL  - 13
IS  - 12
SP  - 12340
EP  - 12348
DO  - 10.20964/2018.12.59
ER  - 
@article{
author = "Lović, Jelena and Stevanović, Sanja and Anđelković, Boban D. and Petrovic, S. and Vukovic, D. and Prlainović, Nevena Ž. and Mijin, Dušan and Nikolić, Nebojša D. and Avramov Ivić, Milka",
year = "2018",
abstract = "Glucose biosensor containing cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is constructed and already electrochemically tested. Now, the electrochemical behavior of biosensor in human serum is further investigated and supported by morphological characterization of layers for the first time. The morphology and microstructure of layers was examined by Fourier transformed infra red spectroscopy (FTIR), atomic force (AFM) and optical microscopy (OM). The electrochemical indication that the Cys-GA-GOx film on Au surface is not compact and that there were some bare regions which remain catalytically active is supported by AFM and OM results. The construction and the nature of bonding of Au-Cys-GA-GOx biosensor layers is confirmed by the FTIR study.",
publisher = "Esg, Belgrade",
journal = "International Journal of Electrochemical Science",
title = "Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode",
volume = "13",
number = "12",
pages = "12340-12348",
doi = "10.20964/2018.12.59"
}
Lović, J., Stevanović, S., Anđelković, B. D., Petrovic, S., Vukovic, D., Prlainović, N. Ž., Mijin, D., Nikolić, N. D.,& Avramov Ivić, M.. (2018). Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode. in International Journal of Electrochemical Science
Esg, Belgrade., 13(12), 12340-12348.
https://doi.org/10.20964/2018.12.59
Lović J, Stevanović S, Anđelković BD, Petrovic S, Vukovic D, Prlainović NŽ, Mijin D, Nikolić ND, Avramov Ivić M. Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode. in International Journal of Electrochemical Science. 2018;13(12):12340-12348.
doi:10.20964/2018.12.59 .
Lović, Jelena, Stevanović, Sanja, Anđelković, Boban D., Petrovic, S., Vukovic, D., Prlainović, Nevena Ž., Mijin, Dušan, Nikolić, Nebojša D., Avramov Ivić, Milka, "Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode" in International Journal of Electrochemical Science, 13, no. 12 (2018):12340-12348,
https://doi.org/10.20964/2018.12.59 . .

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