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Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode

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2018
131212340.pdf (737.0Kb)
Authors
Lović, Jelena
Stevanović, Sanja
Anđelković, Boban D.
Petrovic, S.
Vukovic, D.
Prlainović, Nevena Ž.
Mijin, Dušan
Nikolić, Nebojša D.
Avramov Ivić, Milka
Article (Published version)
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Abstract
Glucose biosensor containing cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is constructed and already electrochemically tested. Now, the electrochemical behavior of biosensor in human serum is further investigated and supported by morphological characterization of layers for the first time. The morphology and microstructure of layers was examined by Fourier transformed infra red spectroscopy (FTIR), atomic force (AFM) and optical microscopy (OM). The electrochemical indication that the Cys-GA-GOx film on Au surface is not compact and that there were some bare regions which remain catalytically active is supported by AFM and OM results. The construction and the nature of bonding of Au-Cys-GA-GOx biosensor layers is confirmed by the FTIR study.
Keywords:
Biosensor / Electrochemical glucose sensing / Microstructural characterization / FTIR / AFM / OM microstructural characterization
Source:
International Journal of Electrochemical Science, 2018, 13, 12, 12340-12348
Publisher:
  • Esg, Belgrade
Projects:
  • Study of the Synthesis, Structure and Activity of Natural and Synthetic Organic Compounds (RS-172013)
  • New approach in designing materials for energy conversion and energy storage systems (RS-172060)

DOI: 10.20964/2018.12.59

ISSN: 1452-3981

WoS: 000452744500080

Scopus: 2-s2.0-85058157122
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URI
http://cer.ihtm.bg.ac.rs/handle/123456789/2364
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