Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode
Authors
Lović, Jelena
Stevanović, Sanja

Anđelković, Boban D.

Petrovic, S.
Vukovic, D.
Prlainović, Nevena Ž.

Mijin, Dušan

Nikolić, Nebojša D.

Avramov Ivić, Milka

Article (Published version)
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Show full item recordAbstract
Glucose biosensor containing cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is constructed and already electrochemically tested. Now, the electrochemical behavior of biosensor in human serum is further investigated and supported by morphological characterization of layers for the first time. The morphology and microstructure of layers was examined by Fourier transformed infra red spectroscopy (FTIR), atomic force (AFM) and optical microscopy (OM). The electrochemical indication that the Cys-GA-GOx film on Au surface is not compact and that there were some bare regions which remain catalytically active is supported by AFM and OM results. The construction and the nature of bonding of Au-Cys-GA-GOx biosensor layers is confirmed by the FTIR study.
Keywords:
Biosensor / Electrochemical glucose sensing / Microstructural characterization / FTIR / AFM / OM microstructural characterizationSource:
International Journal of Electrochemical Science, 2018, 13, 12, 12340-12348Publisher:
- Esg, Belgrade
Funding / projects:
- Study of the Synthesis, Structure and Activity of Natural and Synthetic Organic Compounds (RS-172013)
- New approach in designing materials for energy conversion and energy storage systems (RS-172060)
DOI: 10.20964/2018.12.59
ISSN: 1452-3981
WoS: 000452744500080
Scopus: 2-s2.0-85058157122
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IHTMTY - JOUR AU - Lović, Jelena AU - Stevanović, Sanja AU - Anđelković, Boban D. AU - Petrovic, S. AU - Vukovic, D. AU - Prlainović, Nevena Ž. AU - Mijin, Dušan AU - Nikolić, Nebojša D. AU - Avramov Ivić, Milka PY - 2018 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/2364 AB - Glucose biosensor containing cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is constructed and already electrochemically tested. Now, the electrochemical behavior of biosensor in human serum is further investigated and supported by morphological characterization of layers for the first time. The morphology and microstructure of layers was examined by Fourier transformed infra red spectroscopy (FTIR), atomic force (AFM) and optical microscopy (OM). The electrochemical indication that the Cys-GA-GOx film on Au surface is not compact and that there were some bare regions which remain catalytically active is supported by AFM and OM results. The construction and the nature of bonding of Au-Cys-GA-GOx biosensor layers is confirmed by the FTIR study. PB - Esg, Belgrade T2 - International Journal of Electrochemical Science T1 - Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode VL - 13 IS - 12 SP - 12340 EP - 12348 DO - 10.20964/2018.12.59 ER -
@article{ author = "Lović, Jelena and Stevanović, Sanja and Anđelković, Boban D. and Petrovic, S. and Vukovic, D. and Prlainović, Nevena Ž. and Mijin, Dušan and Nikolić, Nebojša D. and Avramov Ivić, Milka", year = "2018", abstract = "Glucose biosensor containing cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is constructed and already electrochemically tested. Now, the electrochemical behavior of biosensor in human serum is further investigated and supported by morphological characterization of layers for the first time. The morphology and microstructure of layers was examined by Fourier transformed infra red spectroscopy (FTIR), atomic force (AFM) and optical microscopy (OM). The electrochemical indication that the Cys-GA-GOx film on Au surface is not compact and that there were some bare regions which remain catalytically active is supported by AFM and OM results. The construction and the nature of bonding of Au-Cys-GA-GOx biosensor layers is confirmed by the FTIR study.", publisher = "Esg, Belgrade", journal = "International Journal of Electrochemical Science", title = "Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode", volume = "13", number = "12", pages = "12340-12348", doi = "10.20964/2018.12.59" }
Lović, J., Stevanović, S., Anđelković, B. D., Petrovic, S., Vukovic, D., Prlainović, N. Ž., Mijin, D., Nikolić, N. D.,& Avramov Ivić, M.. (2018). Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode. in International Journal of Electrochemical Science Esg, Belgrade., 13(12), 12340-12348. https://doi.org/10.20964/2018.12.59
Lović J, Stevanović S, Anđelković BD, Petrovic S, Vukovic D, Prlainović NŽ, Mijin D, Nikolić ND, Avramov Ivić M. Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode. in International Journal of Electrochemical Science. 2018;13(12):12340-12348. doi:10.20964/2018.12.59 .
Lović, Jelena, Stevanović, Sanja, Anđelković, Boban D., Petrovic, S., Vukovic, D., Prlainović, Nevena Ž., Mijin, Dušan, Nikolić, Nebojša D., Avramov Ivić, Milka, "Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode" in International Journal of Electrochemical Science, 13, no. 12 (2018):12340-12348, https://doi.org/10.20964/2018.12.59 . .