Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai

2018
Authors
Izrael‑Živković, LidijaRikalović, Milena

Gojgić-Cvijović, Gordana

Kazazic, Sasa
Vrvić, Miroslav

Brčeski, Ilija

Beškoski, Vladimir

Lončarević, Branka

Gopcevic, Kristina
Karadžić, Ivanka

Article (Published version)
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Pseudomonas aeruginosa san ai is a promising candidate for bioremediation of cadmium pollution, as it resists a high concentration of up to 7.2 mM of cadmium. Leaving biomass of P. aeruginosa san ai exposed to cadmium has a large biosorption potential, implying its capacity to extract heavy metal from contaminated medium. In the present study, we investigated tolerance and accumulation of cadmium on protein level by shotgun proteomics approach based on liquid chromatography and tandem mass spectrometry coupled with bioinformatics to identify proteins. Size exclusion chromatography was used for protein prefractionation to preserve native forms of metalloproteins and protein complexes. Using this approach a total of 60 proteins were observed as up-regulated in cadmium-amended culture. Almost a third of the total numbers of up-regulated were metalloproteins. Particularly interesting are denitrification proteins which are over expressed but not active, suggesting their protective role in c...onditions of heavy metal exposure. P. aeruginosa san ai developed a complex mechanism to adapt to cadmium, based on: extracellular biosorption, bioaccumulation, the formation of biofilm, controlled siderophore production, enhanced respiration and modified protein profile. An increased abundance of proteins involved in: cell energy metabolism, including denitrification proteins; amino acid metabolism; cell motility and posttranslational modifications, primarily based on thiol-disulfide exchange, were observed. Enhanced oxygen consumption of biomass in cadmium-amended culture versus control was found. Our results signify that P. aeruginosa san ai is naturally well equipped to overcome and survive high doses of cadmium and, as such, has a great potential for application in bioremediation of cadmium polluted sites.
Source:
RSC Advances, 2018, 8, 19, 10549-10560Publisher:
- Royal Soc Chemistry, Cambridge
Funding / projects:
DOI: 10.1039/c8ra00371h
ISSN: 2046-2069
WoS: 000428581000023
Scopus: 2-s2.0-85044180168
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IHTMTY - JOUR AU - Izrael‑Živković, Lidija AU - Rikalović, Milena AU - Gojgić-Cvijović, Gordana AU - Kazazic, Sasa AU - Vrvić, Miroslav AU - Brčeski, Ilija AU - Beškoski, Vladimir AU - Lončarević, Branka AU - Gopcevic, Kristina AU - Karadžić, Ivanka PY - 2018 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/2347 AB - Pseudomonas aeruginosa san ai is a promising candidate for bioremediation of cadmium pollution, as it resists a high concentration of up to 7.2 mM of cadmium. Leaving biomass of P. aeruginosa san ai exposed to cadmium has a large biosorption potential, implying its capacity to extract heavy metal from contaminated medium. In the present study, we investigated tolerance and accumulation of cadmium on protein level by shotgun proteomics approach based on liquid chromatography and tandem mass spectrometry coupled with bioinformatics to identify proteins. Size exclusion chromatography was used for protein prefractionation to preserve native forms of metalloproteins and protein complexes. Using this approach a total of 60 proteins were observed as up-regulated in cadmium-amended culture. Almost a third of the total numbers of up-regulated were metalloproteins. Particularly interesting are denitrification proteins which are over expressed but not active, suggesting their protective role in conditions of heavy metal exposure. P. aeruginosa san ai developed a complex mechanism to adapt to cadmium, based on: extracellular biosorption, bioaccumulation, the formation of biofilm, controlled siderophore production, enhanced respiration and modified protein profile. An increased abundance of proteins involved in: cell energy metabolism, including denitrification proteins; amino acid metabolism; cell motility and posttranslational modifications, primarily based on thiol-disulfide exchange, were observed. Enhanced oxygen consumption of biomass in cadmium-amended culture versus control was found. Our results signify that P. aeruginosa san ai is naturally well equipped to overcome and survive high doses of cadmium and, as such, has a great potential for application in bioremediation of cadmium polluted sites. PB - Royal Soc Chemistry, Cambridge T2 - RSC Advances T1 - Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai VL - 8 IS - 19 SP - 10549 EP - 10560 DO - 10.1039/c8ra00371h ER -
@article{ author = "Izrael‑Živković, Lidija and Rikalović, Milena and Gojgić-Cvijović, Gordana and Kazazic, Sasa and Vrvić, Miroslav and Brčeski, Ilija and Beškoski, Vladimir and Lončarević, Branka and Gopcevic, Kristina and Karadžić, Ivanka", year = "2018", abstract = "Pseudomonas aeruginosa san ai is a promising candidate for bioremediation of cadmium pollution, as it resists a high concentration of up to 7.2 mM of cadmium. Leaving biomass of P. aeruginosa san ai exposed to cadmium has a large biosorption potential, implying its capacity to extract heavy metal from contaminated medium. In the present study, we investigated tolerance and accumulation of cadmium on protein level by shotgun proteomics approach based on liquid chromatography and tandem mass spectrometry coupled with bioinformatics to identify proteins. Size exclusion chromatography was used for protein prefractionation to preserve native forms of metalloproteins and protein complexes. Using this approach a total of 60 proteins were observed as up-regulated in cadmium-amended culture. Almost a third of the total numbers of up-regulated were metalloproteins. Particularly interesting are denitrification proteins which are over expressed but not active, suggesting their protective role in conditions of heavy metal exposure. P. aeruginosa san ai developed a complex mechanism to adapt to cadmium, based on: extracellular biosorption, bioaccumulation, the formation of biofilm, controlled siderophore production, enhanced respiration and modified protein profile. An increased abundance of proteins involved in: cell energy metabolism, including denitrification proteins; amino acid metabolism; cell motility and posttranslational modifications, primarily based on thiol-disulfide exchange, were observed. Enhanced oxygen consumption of biomass in cadmium-amended culture versus control was found. Our results signify that P. aeruginosa san ai is naturally well equipped to overcome and survive high doses of cadmium and, as such, has a great potential for application in bioremediation of cadmium polluted sites.", publisher = "Royal Soc Chemistry, Cambridge", journal = "RSC Advances", title = "Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai", volume = "8", number = "19", pages = "10549-10560", doi = "10.1039/c8ra00371h" }
Izrael‑Živković, L., Rikalović, M., Gojgić-Cvijović, G., Kazazic, S., Vrvić, M., Brčeski, I., Beškoski, V., Lončarević, B., Gopcevic, K.,& Karadžić, I.. (2018). Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai. in RSC Advances Royal Soc Chemistry, Cambridge., 8(19), 10549-10560. https://doi.org/10.1039/c8ra00371h
Izrael‑Živković L, Rikalović M, Gojgić-Cvijović G, Kazazic S, Vrvić M, Brčeski I, Beškoski V, Lončarević B, Gopcevic K, Karadžić I. Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai. in RSC Advances. 2018;8(19):10549-10560. doi:10.1039/c8ra00371h .
Izrael‑Živković, Lidija, Rikalović, Milena, Gojgić-Cvijović, Gordana, Kazazic, Sasa, Vrvić, Miroslav, Brčeski, Ilija, Beškoski, Vladimir, Lončarević, Branka, Gopcevic, Kristina, Karadžić, Ivanka, "Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai" in RSC Advances, 8, no. 19 (2018):10549-10560, https://doi.org/10.1039/c8ra00371h . .