The genus Amphoricarpos Vis. is endemic of westpart of Balkan peninsula. In order to get more insight into the ambiguous taxonomy of the genus, the metabolomic study of the Amphoricarpos samples, has been undertaken. The(1)H NMR spectroscopy combined with multivariate data analysis has been applied. OPLS-DA has been shown to be the best method for clear discrimination of these samples based on the metabolites present in the extracts. The main difference between A. autariatus and A. neumayeri has been found to be the presence of characteristic sesquiterpene lactones of guaianolide type named amphoricarpolides. While the main sesquiterpene lactone in A. neumayeri was oxidized in position 2, those present in both subspecies of A. autariatus samples were oxidized in position 9. The use of sesquiterpene lactones as taxonomic characters for differentiating species of the Asteraceae family was reviewed elsewhere. For the differentiation of two subspecies, A. autariatus ssp. autariatus, and A.... autariatus ssp. bertisceus, chlorogenic and malic acid have been found to be decisive, but these compounds do not have chemotaxonomic significance. Our results were in accordance with above mentioned classification of Blecic and Mayer but they differentiate from those of the recent genomic study of the genus indicating a single, genetically, morphologically and ecologically variable species, without intraspecific taxa.
TY - JOUR
AU - Cvetković, Mirjana
AU - Anđelković, Boban D.
AU - Stevanovic, Vladimir
AU - Jadranin, Milka
AU - Đorđević, Iris
AU - Tešević, Vele
AU - Milosavljević, Slobodan
AU - Gođevac, Dejan
PY - 2018
UR - http://cer.ihtm.bg.ac.rs/handle/123456789/2316
AB - The genus Amphoricarpos Vis. is endemic of westpart of Balkan peninsula. In order to get more insight into the ambiguous taxonomy of the genus, the metabolomic study of the Amphoricarpos samples, has been undertaken. The(1)H NMR spectroscopy combined with multivariate data analysis has been applied. OPLS-DA has been shown to be the best method for clear discrimination of these samples based on the metabolites present in the extracts. The main difference between A. autariatus and A. neumayeri has been found to be the presence of characteristic sesquiterpene lactones of guaianolide type named amphoricarpolides. While the main sesquiterpene lactone in A. neumayeri was oxidized in position 2, those present in both subspecies of A. autariatus samples were oxidized in position 9. The use of sesquiterpene lactones as taxonomic characters for differentiating species of the Asteraceae family was reviewed elsewhere. For the differentiation of two subspecies, A. autariatus ssp. autariatus, and A. autariatus ssp. bertisceus, chlorogenic and malic acid have been found to be decisive, but these compounds do not have chemotaxonomic significance. Our results were in accordance with above mentioned classification of Blecic and Mayer but they differentiate from those of the recent genomic study of the genus indicating a single, genetically, morphologically and ecologically variable species, without intraspecific taxa.
PB - Elsevier Science Bv, Amsterdam
T2 - Phytochemistry Letters
T1 - NMR-based metabolomics study of Amphoricarpos species from Montenegro
VL - 25
SP - 1
EP - 5
DO - 10.1016/j.phytol.2018.03.013
ER -
@article{
author = "Cvetković, Mirjana and Anđelković, Boban D. and Stevanovic, Vladimir and Jadranin, Milka and Đorđević, Iris and Tešević, Vele and Milosavljević, Slobodan and Gođevac, Dejan",
year = "2018",
url = "http://cer.ihtm.bg.ac.rs/handle/123456789/2316",
abstract = "The genus Amphoricarpos Vis. is endemic of westpart of Balkan peninsula. In order to get more insight into the ambiguous taxonomy of the genus, the metabolomic study of the Amphoricarpos samples, has been undertaken. The(1)H NMR spectroscopy combined with multivariate data analysis has been applied. OPLS-DA has been shown to be the best method for clear discrimination of these samples based on the metabolites present in the extracts. The main difference between A. autariatus and A. neumayeri has been found to be the presence of characteristic sesquiterpene lactones of guaianolide type named amphoricarpolides. While the main sesquiterpene lactone in A. neumayeri was oxidized in position 2, those present in both subspecies of A. autariatus samples were oxidized in position 9. The use of sesquiterpene lactones as taxonomic characters for differentiating species of the Asteraceae family was reviewed elsewhere. For the differentiation of two subspecies, A. autariatus ssp. autariatus, and A. autariatus ssp. bertisceus, chlorogenic and malic acid have been found to be decisive, but these compounds do not have chemotaxonomic significance. Our results were in accordance with above mentioned classification of Blecic and Mayer but they differentiate from those of the recent genomic study of the genus indicating a single, genetically, morphologically and ecologically variable species, without intraspecific taxa.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Phytochemistry Letters",
title = "NMR-based metabolomics study of Amphoricarpos species from Montenegro",
volume = "25",
pages = "1-5",
doi = "10.1016/j.phytol.2018.03.013"
}
Cvetković, M., Anđelković, B. D., Stevanovic, V., Jadranin, M., Đorđević, I., Tešević, V., Milosavljević, S.,& Gođevac, D. (2018). NMR-based metabolomics study of Amphoricarpos species from Montenegro.
Phytochemistry Letters
Elsevier Science Bv, Amsterdam., 25, 1-5.
https://doi.org/10.1016/j.phytol.2018.03.013
Cvetković M, Anđelković BD, Stevanovic V, Jadranin M, Đorđević I, Tešević V, Milosavljević S, Gođevac D. NMR-based metabolomics study of Amphoricarpos species from Montenegro. Phytochemistry Letters. 2018;25:1-5
Cvetković Mirjana, Anđelković Boban D., Stevanovic Vladimir, Jadranin Milka, Đorđević Iris, Tešević Vele, Milosavljević Slobodan, Gođevac Dejan, "NMR-based metabolomics study of Amphoricarpos species from Montenegro" Phytochemistry Letters, 25 (2018):1-5,
https://doi.org/10.1016/j.phytol.2018.03.013 .
