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Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts

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2018
Authors
Aleksić, Ivana
Ristivojevic, Petar
Pavić, Aleksandar
Radojevic, Ivana
Čomić, Ljiljana R.
Vasiljevic, Branka
Opsenica, Dejan
Milojković-Opsenica, Dušanka
Senerovic, Lidija
Article (Published version)
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Abstract
Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composit...ion of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.

Keywords:
Trapa natans / Pseudomonas aeruginosa / Quorum sensing / Toxicity / Zebrafish
Source:
Journal of Ethnopharmacology, 2018, 222, 148-158
Publisher:
  • Elsevier Ireland Ltd, Clare
Projects:
  • Microbial diversity study and characterization of beneficial environmental microorganisms (RS-173048)
  • The synthesis of aminoquinoline-based antimalarials and botulinum neurotoxin A inhibitors (RS-172008)
  • Structure-properties relationships of natural and synthetic molecules and their metal complexes (RS-172017)
  • Preclinical investigation of bioactive substances (RS-41010)
Note:
  • The peer-reviewed version: http://cer.ihtm.bg.ac.rs/handle/123456789/3139

DOI: 10.1016/j.jep.2018.05.005

ISSN: 0378-8741

PubMed: 29733941

WoS: 000436224700014

Scopus: 2-s2.0-85046784871
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URI
http://cer.ihtm.bg.ac.rs/handle/123456789/2310
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