Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models
Samo za registrovane korisnike
2017
Autori
Kolarević, Stoimir M.Milovanović, Dragana
Kračun-Kolarević, Margareta
Kostić, Jovana
Sunjog, K.
Martinović, Rajko
Đorđević, Jelena
Novaković, Irena
Sladić, Dušan
Vuković-Gačić, Branka
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In this study, mutagenic and genotoxic potential of anti-tumor compounds avarol, avarone, and its derivatives 3′-methoxyavarone, 4′-(methylamino)avarone and 3′-(methylamino)avarone was evaluated and compared to cytostatics commonly used in chemotherapy (5-fluorouracil, etoposid, and cisplatin). Mutagenic potential of selected hydroquinone and quinones was assessed in prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002. Genotoxic potential was also assessed in eukaryotic models using comet assay in human fetal lung cell line (MRC-5), human adenocarcinoma epithelial cell line (A549), and in human peripheral blood cells (HPBC). The results indicated that avarol and avarone do not exert mutagenic/genotoxic potential. Among the studied avarone derivatives, mutagenic potential was detected by SOS/umuC test for 3′-(methylamino)avarone, but only after metabolic activation. The results of comet assay indicated that 3′-methoxyavarone and 3′-(methylamino)avarone have ...a significant impact on the level of DNA damage in the MRC-5 cell line. Genotoxic potential was not observed in A549 cells or HPBC probably due to a different uptake rate for the compounds and lower in metabolism rate within these cells.
Ključne reči:
Avarol / avarone / comet assay / genotoxicity / mutagenicity / SOS/umuCIzvor:
Drug and Chemical Toxicology, 2017, 1-10Izdavač:
- Taylor and Francis Ltd
Finansiranje / projekti:
- Interakcije prirodnih proizvoda, njihovih derivata i kompleksnih jedinjenja sa proteinima i nukleinskim kiselinama (RS-172055)
- Biološki aktivni prirodni proizvodi kao potencijalni izvori novih lekova i dijetetskih suplemenata (RS-172058)
DOI: 10.1080/01480545.2017.1413108
ISSN: 0148-0545
WoS: 000459818600003
Scopus: 2-s2.0-85041102861
Institucija/grupa
IHTMTY - JOUR AU - Kolarević, Stoimir M. AU - Milovanović, Dragana AU - Kračun-Kolarević, Margareta AU - Kostić, Jovana AU - Sunjog, K. AU - Martinović, Rajko AU - Đorđević, Jelena AU - Novaković, Irena AU - Sladić, Dušan AU - Vuković-Gačić, Branka PY - 2017 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/2232 AB - In this study, mutagenic and genotoxic potential of anti-tumor compounds avarol, avarone, and its derivatives 3′-methoxyavarone, 4′-(methylamino)avarone and 3′-(methylamino)avarone was evaluated and compared to cytostatics commonly used in chemotherapy (5-fluorouracil, etoposid, and cisplatin). Mutagenic potential of selected hydroquinone and quinones was assessed in prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002. Genotoxic potential was also assessed in eukaryotic models using comet assay in human fetal lung cell line (MRC-5), human adenocarcinoma epithelial cell line (A549), and in human peripheral blood cells (HPBC). The results indicated that avarol and avarone do not exert mutagenic/genotoxic potential. Among the studied avarone derivatives, mutagenic potential was detected by SOS/umuC test for 3′-(methylamino)avarone, but only after metabolic activation. The results of comet assay indicated that 3′-methoxyavarone and 3′-(methylamino)avarone have a significant impact on the level of DNA damage in the MRC-5 cell line. Genotoxic potential was not observed in A549 cells or HPBC probably due to a different uptake rate for the compounds and lower in metabolism rate within these cells. PB - Taylor and Francis Ltd T2 - Drug and Chemical Toxicology T1 - Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models SP - 1 EP - 10 DO - 10.1080/01480545.2017.1413108 ER -
@article{ author = "Kolarević, Stoimir M. and Milovanović, Dragana and Kračun-Kolarević, Margareta and Kostić, Jovana and Sunjog, K. and Martinović, Rajko and Đorđević, Jelena and Novaković, Irena and Sladić, Dušan and Vuković-Gačić, Branka", year = "2017", abstract = "In this study, mutagenic and genotoxic potential of anti-tumor compounds avarol, avarone, and its derivatives 3′-methoxyavarone, 4′-(methylamino)avarone and 3′-(methylamino)avarone was evaluated and compared to cytostatics commonly used in chemotherapy (5-fluorouracil, etoposid, and cisplatin). Mutagenic potential of selected hydroquinone and quinones was assessed in prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002. Genotoxic potential was also assessed in eukaryotic models using comet assay in human fetal lung cell line (MRC-5), human adenocarcinoma epithelial cell line (A549), and in human peripheral blood cells (HPBC). The results indicated that avarol and avarone do not exert mutagenic/genotoxic potential. Among the studied avarone derivatives, mutagenic potential was detected by SOS/umuC test for 3′-(methylamino)avarone, but only after metabolic activation. The results of comet assay indicated that 3′-methoxyavarone and 3′-(methylamino)avarone have a significant impact on the level of DNA damage in the MRC-5 cell line. Genotoxic potential was not observed in A549 cells or HPBC probably due to a different uptake rate for the compounds and lower in metabolism rate within these cells.", publisher = "Taylor and Francis Ltd", journal = "Drug and Chemical Toxicology", title = "Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models", pages = "1-10", doi = "10.1080/01480545.2017.1413108" }
Kolarević, S. M., Milovanović, D., Kračun-Kolarević, M., Kostić, J., Sunjog, K., Martinović, R., Đorđević, J., Novaković, I., Sladić, D.,& Vuković-Gačić, B.. (2017). Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models. in Drug and Chemical Toxicology Taylor and Francis Ltd., 1-10. https://doi.org/10.1080/01480545.2017.1413108
Kolarević SM, Milovanović D, Kračun-Kolarević M, Kostić J, Sunjog K, Martinović R, Đorđević J, Novaković I, Sladić D, Vuković-Gačić B. Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models. in Drug and Chemical Toxicology. 2017;:1-10. doi:10.1080/01480545.2017.1413108 .
Kolarević, Stoimir M., Milovanović, Dragana, Kračun-Kolarević, Margareta, Kostić, Jovana, Sunjog, K., Martinović, Rajko, Đorđević, Jelena, Novaković, Irena, Sladić, Dušan, Vuković-Gačić, Branka, "Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models" in Drug and Chemical Toxicology (2017):1-10, https://doi.org/10.1080/01480545.2017.1413108 . .