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dc.creatorKaluđerović, Goran N.
dc.creatorBulatović, Mirna
dc.creatorKrajnović, Tamara T.
dc.creatorPaschke, Reinhard
dc.creatorZmejkovski, Bojana
dc.creatorMaksimović-Ivanić, Danijela D.
dc.creatorMijatović, Sanja
dc.date.accessioned2019-01-30T17:55:54Z
dc.date.available2019-01-30T17:55:54Z
dc.date.issued2017
dc.identifier.issn2304-6740
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/2222
dc.description.abstractSynthesis of platinum(II) conjugate with acetylated betulinic acid tris(hydroxymethyl) aminomethane ester (BATRIS) is presented (BATRISPt). HR-ESI-MS and multinuclear NMR spectroscopy, as well as elemental analysis were used for characterization of BATRISPt. Cytotoxicity (3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), crystal violet (CV), and sulforhodamine B (SRB) assays) of BA, BATRIS, BATRISPt, and cisplatin were assessed on seven different tumor cell lines: melanoma B16, colon HCT116 and DLD-1, adenocarcinoma HeLa, breast MCF-7, and anaplastic thyroid tumor 8505C and SW1736; as well as normal MRC-5 fibroblasts. Furthermore, the effect of the mentioned compounds on the apoptosis (Annexin V/PI assay) and autophagy induction (acridine orange (AO) assay) as well as caspase 3, 8, and 9 activation were investigated on the selected B16 melanoma cell line. BATRISPt showed lower activity than BA, BATRIS, or cisplatin. All tested compounds triggered apoptosis in B16 cells. Induction of autophagy was observed in B16 cells exposed only to BATRIS. On the other hand, new conjugate activates caspases 8 and 9 in B16 cells with higher impact than BATRIS or cisplatin alone.en
dc.publisherMDPI AG
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172035/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173013/RS//
dc.rightsopenAccess
dc.sourceInorganics
dc.subjectApoptosisen
dc.subjectAutophagyen
dc.subjectBetulinic aciden
dc.subjectCisplatinen
dc.subjectDrug conjugateen
dc.title(18-Crown-6)potassium(I) trichlorido[28-acetyl-3- (tris-(hydroxylmethyl)amino-ethane)betulinic ester-κN]platinum(II): Synthesis and in vitro antitumor activityen
dc.typearticle
dc.rights.licenseBY
dcterms.abstractБулатовић, М.; Максимовић-Иванић, Д.; Мијатовић, С.; Змејковски, Бојана; Калудеровић, Г.Н.; Пасцхке, Р.; Крајновић, Т.;
dc.citation.volume5
dc.citation.issue3
dc.citation.other5(3):
dc.identifier.doi10.3390/inorganics5030056
dc.identifier.rcubConv_4453
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs//bitstream/id/8622/2220.pdf
dc.identifier.scopus2-s2.0-85045409497
dc.identifier.wos000412058700017
dc.type.versionpublishedVersion


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