Anti-hydroxyl radical activity, redox potential and anti-AChE activity of Amanita strobiliformis polysaccharide extract
Abstract
This study outlines antioxidant and anti-AChE activities of the polysaccharide (PSH) extract from the mushroom species Amanita strobiliformis. Both the presence of α and ß glucans within the aforementioned extract was recorded. PSH extract displayed a profound scavenging activity of OH radicals (IC50 value, 11.86 ± 0.59 μg/mL) and high potential for reduction of Fe3+ ions (174.11 ± 8.70 mg eq. AA/g d.w.) being almost 48- and 5-fold more effective than mannitol and butylated hydroxytoluene used as a positive control, respectively. Compared with galanthamine (0.001 μg), the same extract exhibited a moderate anti-AChE activity (10 μg) in solid. Since purified PSH extract exhibited higher bioactivity (IC50 value 7.27 ± 0.31 μg/mL, 197.68 ± 9.47 mg eq. AA/g d.w. and 0.1 μg, respectively), it can be predominantly ascribed to the polysaccharide compounds. A. strobiliformis PSH extract may be considered as a promising resource of potent bioactive polysaccharides of natural origin successfully ...addressing both oxidative stress and lack of acetylcholine.
Keywords:
Amanitaceae / biological activity / Mushrooms / polar extractSource:
Natural Product Research, 2017, 1-5Publisher:
- Taylor and Francis Ltd.
Funding / projects:
- Biosensing Technologies and Global System for Long-Term Research and Integrated Management of Ecosystems (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43002)
- Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43004)
- Natural products of wild, cultivated and edible plants: structure and bioactivity determination (RS-MESTD-Basic Research (BR or ON)-172053)
DOI: 10.1080/14786419.2017.1422183
ISSN: 1478-6419
WoS: 000469243700024
Scopus: 2-s2.0-85039854192
Collections
Institution/Community
IHTMTY - JOUR AU - Karaman, M. AU - Janjušević, L. AU - Jakovljević, Dragica AU - Šibul, F. AU - Pejin, Boris PY - 2017 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/2214 AB - This study outlines antioxidant and anti-AChE activities of the polysaccharide (PSH) extract from the mushroom species Amanita strobiliformis. Both the presence of α and ß glucans within the aforementioned extract was recorded. PSH extract displayed a profound scavenging activity of OH radicals (IC50 value, 11.86 ± 0.59 μg/mL) and high potential for reduction of Fe3+ ions (174.11 ± 8.70 mg eq. AA/g d.w.) being almost 48- and 5-fold more effective than mannitol and butylated hydroxytoluene used as a positive control, respectively. Compared with galanthamine (0.001 μg), the same extract exhibited a moderate anti-AChE activity (10 μg) in solid. Since purified PSH extract exhibited higher bioactivity (IC50 value 7.27 ± 0.31 μg/mL, 197.68 ± 9.47 mg eq. AA/g d.w. and 0.1 μg, respectively), it can be predominantly ascribed to the polysaccharide compounds. A. strobiliformis PSH extract may be considered as a promising resource of potent bioactive polysaccharides of natural origin successfully addressing both oxidative stress and lack of acetylcholine. PB - Taylor and Francis Ltd. T2 - Natural Product Research T1 - Anti-hydroxyl radical activity, redox potential and anti-AChE activity of Amanita strobiliformis polysaccharide extract SP - 1 EP - 5 DO - 10.1080/14786419.2017.1422183 ER -
@article{ author = "Karaman, M. and Janjušević, L. and Jakovljević, Dragica and Šibul, F. and Pejin, Boris", year = "2017", abstract = "This study outlines antioxidant and anti-AChE activities of the polysaccharide (PSH) extract from the mushroom species Amanita strobiliformis. Both the presence of α and ß glucans within the aforementioned extract was recorded. PSH extract displayed a profound scavenging activity of OH radicals (IC50 value, 11.86 ± 0.59 μg/mL) and high potential for reduction of Fe3+ ions (174.11 ± 8.70 mg eq. AA/g d.w.) being almost 48- and 5-fold more effective than mannitol and butylated hydroxytoluene used as a positive control, respectively. Compared with galanthamine (0.001 μg), the same extract exhibited a moderate anti-AChE activity (10 μg) in solid. Since purified PSH extract exhibited higher bioactivity (IC50 value 7.27 ± 0.31 μg/mL, 197.68 ± 9.47 mg eq. AA/g d.w. and 0.1 μg, respectively), it can be predominantly ascribed to the polysaccharide compounds. A. strobiliformis PSH extract may be considered as a promising resource of potent bioactive polysaccharides of natural origin successfully addressing both oxidative stress and lack of acetylcholine.", publisher = "Taylor and Francis Ltd.", journal = "Natural Product Research", title = "Anti-hydroxyl radical activity, redox potential and anti-AChE activity of Amanita strobiliformis polysaccharide extract", pages = "1-5", doi = "10.1080/14786419.2017.1422183" }
Karaman, M., Janjušević, L., Jakovljević, D., Šibul, F.,& Pejin, B.. (2017). Anti-hydroxyl radical activity, redox potential and anti-AChE activity of Amanita strobiliformis polysaccharide extract. in Natural Product Research Taylor and Francis Ltd.., 1-5. https://doi.org/10.1080/14786419.2017.1422183
Karaman M, Janjušević L, Jakovljević D, Šibul F, Pejin B. Anti-hydroxyl radical activity, redox potential and anti-AChE activity of Amanita strobiliformis polysaccharide extract. in Natural Product Research. 2017;:1-5. doi:10.1080/14786419.2017.1422183 .
Karaman, M., Janjušević, L., Jakovljević, Dragica, Šibul, F., Pejin, Boris, "Anti-hydroxyl radical activity, redox potential and anti-AChE activity of Amanita strobiliformis polysaccharide extract" in Natural Product Research (2017):1-5, https://doi.org/10.1080/14786419.2017.1422183 . .