Diarylheptanoids from Alnus viridis ssp viridis and Alnus glutinosa: Modulation of Quorum Sensing Activity in Pseudomonas aeruginosa
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2017
Authors
Ilić-Tomić, Tatjana
Soković, Marina

Vojnovic, Sandra
Ciric, Ana
Veljić, Milan

Nikodinović-Runić, Jasmina

Novaković, Miroslav

Article (Published version)

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Diarylheptanoids from the barks of Alnus viridis ssp. viridis (green alder) and Alnus glutinosa (black alder) were explored for anti-quorum sensing activity. Chemicals with anti-quorum sensing activity have recently been examined for antimicrobial applications. The anti-quorum sensing activity of the selected diarylheptanoids was determined using two biosensors, namely Pseudomonas aeruginosa PAO1 and Chromobacterium violaceum CV026. Although all of the investigated compounds negatively influenced the motility of P. aeruginosa PAO1, four were able to inhibit biofilm formation of this human opportunistic pathogen for 40-70%. Three of the diarylheptanoids (3, 4, and 5) negatively influenced the biosynthesis of pyocyanin, which is under the control of quorum sensing. Platyphyllenone (7) and hirsutenone (5) were able to inhibit the biosynthesis of violacein in C. violaceum CV026, with 5 being able to inhibit the synthesis of both biopigments. Only one of the tested diarylheptanoids (1) was ...shown to significantly decrease the production of acyl homoserine lactones (AHL) in P. aeruginosa PAO1, more specifically, production of the long chain N-(3-oxododecanoyl)- l-HSL. On the other side, four diarylheptanoids (2-5) significantly reduced the synthesis of 2-alkyl-4-quinolones, part of the P. aeruginosa quinolone-mediated signaling system. To properly assess therapeutic potential of these compounds, their in vitro antiproliferative effect on normal human lung fibroblasts was determined, with doses affecting cell proliferation between 10 and 100 mu g/mL. This study confirms that the barks of green and black alders are rich source of phytochemicals with a wide range of biological activities that could further be exploited as natural agents against bacterial contaminations and infections.
Keywords:
Alnus viridis ssp viridis / Alnus glutinosa / Betulaceae / diarylheptanoids / quorum sensing / acyl homoserine lactone / Pseudomonas aeruginosaSource:
Planta Medica, 2017, 83, 01-02, 117-125Publisher:
- Georg Thieme Verlag Kg, Stuttgart
Funding / projects:
- Natural products of wild, cultivated and edible plants: structure and bioactivity determination (RS-172053)
- Microbial diversity study and characterization of beneficial environmental microorganisms (RS-173048)
DOI: 10.1055/s-0042-107674
ISSN: 0032-0943
PubMed: 27220074
WoS: 000393182100015
Scopus: 2-s2.0-84969933386
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IHTMTY - JOUR AU - Ilić-Tomić, Tatjana AU - Soković, Marina AU - Vojnovic, Sandra AU - Ciric, Ana AU - Veljić, Milan AU - Nikodinović-Runić, Jasmina AU - Novaković, Miroslav PY - 2017 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/2199 AB - Diarylheptanoids from the barks of Alnus viridis ssp. viridis (green alder) and Alnus glutinosa (black alder) were explored for anti-quorum sensing activity. Chemicals with anti-quorum sensing activity have recently been examined for antimicrobial applications. The anti-quorum sensing activity of the selected diarylheptanoids was determined using two biosensors, namely Pseudomonas aeruginosa PAO1 and Chromobacterium violaceum CV026. Although all of the investigated compounds negatively influenced the motility of P. aeruginosa PAO1, four were able to inhibit biofilm formation of this human opportunistic pathogen for 40-70%. Three of the diarylheptanoids (3, 4, and 5) negatively influenced the biosynthesis of pyocyanin, which is under the control of quorum sensing. Platyphyllenone (7) and hirsutenone (5) were able to inhibit the biosynthesis of violacein in C. violaceum CV026, with 5 being able to inhibit the synthesis of both biopigments. Only one of the tested diarylheptanoids (1) was shown to significantly decrease the production of acyl homoserine lactones (AHL) in P. aeruginosa PAO1, more specifically, production of the long chain N-(3-oxododecanoyl)- l-HSL. On the other side, four diarylheptanoids (2-5) significantly reduced the synthesis of 2-alkyl-4-quinolones, part of the P. aeruginosa quinolone-mediated signaling system. To properly assess therapeutic potential of these compounds, their in vitro antiproliferative effect on normal human lung fibroblasts was determined, with doses affecting cell proliferation between 10 and 100 mu g/mL. This study confirms that the barks of green and black alders are rich source of phytochemicals with a wide range of biological activities that could further be exploited as natural agents against bacterial contaminations and infections. PB - Georg Thieme Verlag Kg, Stuttgart T2 - Planta Medica T1 - Diarylheptanoids from Alnus viridis ssp viridis and Alnus glutinosa: Modulation of Quorum Sensing Activity in Pseudomonas aeruginosa VL - 83 IS - 01-02 SP - 117 EP - 125 DO - 10.1055/s-0042-107674 ER -
@article{ author = "Ilić-Tomić, Tatjana and Soković, Marina and Vojnovic, Sandra and Ciric, Ana and Veljić, Milan and Nikodinović-Runić, Jasmina and Novaković, Miroslav", year = "2017", abstract = "Diarylheptanoids from the barks of Alnus viridis ssp. viridis (green alder) and Alnus glutinosa (black alder) were explored for anti-quorum sensing activity. Chemicals with anti-quorum sensing activity have recently been examined for antimicrobial applications. The anti-quorum sensing activity of the selected diarylheptanoids was determined using two biosensors, namely Pseudomonas aeruginosa PAO1 and Chromobacterium violaceum CV026. Although all of the investigated compounds negatively influenced the motility of P. aeruginosa PAO1, four were able to inhibit biofilm formation of this human opportunistic pathogen for 40-70%. Three of the diarylheptanoids (3, 4, and 5) negatively influenced the biosynthesis of pyocyanin, which is under the control of quorum sensing. Platyphyllenone (7) and hirsutenone (5) were able to inhibit the biosynthesis of violacein in C. violaceum CV026, with 5 being able to inhibit the synthesis of both biopigments. Only one of the tested diarylheptanoids (1) was shown to significantly decrease the production of acyl homoserine lactones (AHL) in P. aeruginosa PAO1, more specifically, production of the long chain N-(3-oxododecanoyl)- l-HSL. On the other side, four diarylheptanoids (2-5) significantly reduced the synthesis of 2-alkyl-4-quinolones, part of the P. aeruginosa quinolone-mediated signaling system. To properly assess therapeutic potential of these compounds, their in vitro antiproliferative effect on normal human lung fibroblasts was determined, with doses affecting cell proliferation between 10 and 100 mu g/mL. This study confirms that the barks of green and black alders are rich source of phytochemicals with a wide range of biological activities that could further be exploited as natural agents against bacterial contaminations and infections.", publisher = "Georg Thieme Verlag Kg, Stuttgart", journal = "Planta Medica", title = "Diarylheptanoids from Alnus viridis ssp viridis and Alnus glutinosa: Modulation of Quorum Sensing Activity in Pseudomonas aeruginosa", volume = "83", number = "01-02", pages = "117-125", doi = "10.1055/s-0042-107674" }
Ilić-Tomić, T., Soković, M., Vojnovic, S., Ciric, A., Veljić, M., Nikodinović-Runić, J.,& Novaković, M.. (2017). Diarylheptanoids from Alnus viridis ssp viridis and Alnus glutinosa: Modulation of Quorum Sensing Activity in Pseudomonas aeruginosa. in Planta Medica Georg Thieme Verlag Kg, Stuttgart., 83(01-02), 117-125. https://doi.org/10.1055/s-0042-107674
Ilić-Tomić T, Soković M, Vojnovic S, Ciric A, Veljić M, Nikodinović-Runić J, Novaković M. Diarylheptanoids from Alnus viridis ssp viridis and Alnus glutinosa: Modulation of Quorum Sensing Activity in Pseudomonas aeruginosa. in Planta Medica. 2017;83(01-02):117-125. doi:10.1055/s-0042-107674 .
Ilić-Tomić, Tatjana, Soković, Marina, Vojnovic, Sandra, Ciric, Ana, Veljić, Milan, Nikodinović-Runić, Jasmina, Novaković, Miroslav, "Diarylheptanoids from Alnus viridis ssp viridis and Alnus glutinosa: Modulation of Quorum Sensing Activity in Pseudomonas aeruginosa" in Planta Medica, 83, no. 01-02 (2017):117-125, https://doi.org/10.1055/s-0042-107674 . .