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Electrochemical behavior of immobilized hemoglobin in alkaline solution

Authorized Users Only
2017
Authors
Jović-Jovičić, Nataša
Mojović, Zorica
Mojović, Miloš
Banković, Predrag
Ajduković, Marija
Milutinović Nikolić, Aleksandra
Jovanović, Dušan M.
Article (Published version)
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Abstract
Glassy carbon electrode was modified with different synthesized hybrid clay-based materials and tested in alkaline solution with and without H2O2. The hybrid materials were obtained by immobilizing hemoglobin (Hb) on acid activated (AA) clay, or on AA clay modified with different sodium dodecyl sul-fate (SDS) loadings. The obtained materials were characterized using DR UV vis and ESR spectroscopy, elemental analysis, and SEM. The characterization confirmed higher degree of hemoglobin incorpora-tion in the presence of SDS. The presence of SDS on the surface of clay particles resulted in the partial oxidation/denaturation of hemoglobin and formation of hemichrome. Cyclic voltammetry was used for the investigation of the electrochemical behavior of immobilized hemoglobin in alkaline solution. Two cathodic peaks at -0.45 V and -0.70 V were recorded and ascribed to the reduction of heme Fe(III)/Fe(II), and formation of HbFe(I) - highly reduced form of hemoglobin respectively. The latter pea...k reflects hemoglobin denaturation. The presence of H2O2 in the alkaline solution increased current intensities corresponding to both peaks (-0.45 V and -0.7 V). Linear response of peak current intensity vs. H2O2 concentration was monitored for all investigated samples within different H2O2 concentration ranges. The AA-SDSI.0-Hb electrode exhibited the highest current response with linear regression equation in the following form: I(mu A)= 7.99 +1.056 x1f1202] (mM) (R=0.996). The limit of detection of 28 mu M was estimated using the 3 sigma method. Different modified electrodes exhibited different degrees of denaturation resistance. The obtained val-ues of Michaelis-Menten constant indicated that prolonged cycling in the presence of SDS increases protein denaturation.

Keywords:
Acid activated clay / Alkaline electrolyte / Hemoglobin / Modified electrode / Sodium dodecyl sulfate
Source:
Applied Surface Science, 2017, 400, 347-354
Publisher:
  • Elsevier
Funding / projects:
  • Nanostructured Functional and Composite Materials in Catalytic and Sorption Processes (RS-45001)
  • Biomarkers in neurodegenerative and malignant processes (RS-41005)

DOI: 10.1016/j.apsusc.2016.12.151

ISSN: 0169-4332

WoS: 000394201800043

Scopus: 2-s2.0-85007609871
[ Google Scholar ]
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URI
https://cer.ihtm.bg.ac.rs/handle/123456789/2196
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - JOUR
AU  - Jović-Jovičić, Nataša
AU  - Mojović, Zorica
AU  - Mojović, Miloš
AU  - Banković, Predrag
AU  - Ajduković, Marija
AU  - Milutinović Nikolić, Aleksandra
AU  - Jovanović, Dušan M.
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2196
AB  - Glassy carbon electrode was modified with different synthesized hybrid clay-based materials and tested in alkaline solution with and without H2O2. The hybrid materials were obtained by immobilizing hemoglobin (Hb) on acid activated (AA) clay, or on AA clay modified with different sodium dodecyl sul-fate (SDS) loadings. The obtained materials were characterized using DR UV vis and ESR spectroscopy, elemental analysis, and SEM. The characterization confirmed higher degree of hemoglobin incorpora-tion in the presence of SDS. The presence of SDS on the surface of clay particles resulted in the partial oxidation/denaturation of hemoglobin and formation of hemichrome. Cyclic voltammetry was used for the investigation of the electrochemical behavior of immobilized hemoglobin in alkaline solution. Two cathodic peaks at -0.45 V and -0.70 V were recorded and ascribed to the reduction of heme Fe(III)/Fe(II), and formation of HbFe(I) - highly reduced form of hemoglobin respectively. The latter peak reflects hemoglobin denaturation. The presence of H2O2 in the alkaline solution increased current intensities corresponding to both peaks (-0.45 V and -0.7 V). Linear response of peak current intensity vs. H2O2 concentration was monitored for all investigated samples within different H2O2 concentration ranges. The AA-SDSI.0-Hb electrode exhibited the highest current response with linear regression equation in the following form: I(mu A)= 7.99 +1.056 x1f1202] (mM) (R=0.996). The limit of detection of 28 mu M was estimated using the 3 sigma method. Different modified electrodes exhibited different degrees of denaturation resistance. The obtained val-ues of Michaelis-Menten constant indicated that prolonged cycling in the presence of SDS increases protein denaturation.
PB  - Elsevier
T2  - Applied Surface Science
T1  - Electrochemical behavior of immobilized hemoglobin in alkaline solution
VL  - 400
SP  - 347
EP  - 354
DO  - 10.1016/j.apsusc.2016.12.151
ER  - 
@article{
author = "Jović-Jovičić, Nataša and Mojović, Zorica and Mojović, Miloš and Banković, Predrag and Ajduković, Marija and Milutinović Nikolić, Aleksandra and Jovanović, Dušan M.",
year = "2017",
abstract = "Glassy carbon electrode was modified with different synthesized hybrid clay-based materials and tested in alkaline solution with and without H2O2. The hybrid materials were obtained by immobilizing hemoglobin (Hb) on acid activated (AA) clay, or on AA clay modified with different sodium dodecyl sul-fate (SDS) loadings. The obtained materials were characterized using DR UV vis and ESR spectroscopy, elemental analysis, and SEM. The characterization confirmed higher degree of hemoglobin incorpora-tion in the presence of SDS. The presence of SDS on the surface of clay particles resulted in the partial oxidation/denaturation of hemoglobin and formation of hemichrome. Cyclic voltammetry was used for the investigation of the electrochemical behavior of immobilized hemoglobin in alkaline solution. Two cathodic peaks at -0.45 V and -0.70 V were recorded and ascribed to the reduction of heme Fe(III)/Fe(II), and formation of HbFe(I) - highly reduced form of hemoglobin respectively. The latter peak reflects hemoglobin denaturation. The presence of H2O2 in the alkaline solution increased current intensities corresponding to both peaks (-0.45 V and -0.7 V). Linear response of peak current intensity vs. H2O2 concentration was monitored for all investigated samples within different H2O2 concentration ranges. The AA-SDSI.0-Hb electrode exhibited the highest current response with linear regression equation in the following form: I(mu A)= 7.99 +1.056 x1f1202] (mM) (R=0.996). The limit of detection of 28 mu M was estimated using the 3 sigma method. Different modified electrodes exhibited different degrees of denaturation resistance. The obtained val-ues of Michaelis-Menten constant indicated that prolonged cycling in the presence of SDS increases protein denaturation.",
publisher = "Elsevier",
journal = "Applied Surface Science",
title = "Electrochemical behavior of immobilized hemoglobin in alkaline solution",
volume = "400",
pages = "347-354",
doi = "10.1016/j.apsusc.2016.12.151"
}
Jović-Jovičić, N., Mojović, Z., Mojović, M., Banković, P., Ajduković, M., Milutinović Nikolić, A.,& Jovanović, D. M.. (2017). Electrochemical behavior of immobilized hemoglobin in alkaline solution. in Applied Surface Science
Elsevier., 400, 347-354.
https://doi.org/10.1016/j.apsusc.2016.12.151
Jović-Jovičić N, Mojović Z, Mojović M, Banković P, Ajduković M, Milutinović Nikolić A, Jovanović DM. Electrochemical behavior of immobilized hemoglobin in alkaline solution. in Applied Surface Science. 2017;400:347-354.
doi:10.1016/j.apsusc.2016.12.151 .
Jović-Jovičić, Nataša, Mojović, Zorica, Mojović, Miloš, Banković, Predrag, Ajduković, Marija, Milutinović Nikolić, Aleksandra, Jovanović, Dušan M., "Electrochemical behavior of immobilized hemoglobin in alkaline solution" in Applied Surface Science, 400 (2017):347-354,
https://doi.org/10.1016/j.apsusc.2016.12.151 . .

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