Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode

2017
Authors
Lović, Jelena
Stevanović, Sanja

Nikolić, Nebojša D.

Petrovic, S.
Vukovic, D.
Prlainović, Nevena Ž.

Mijin, Dušan

Avramov Ivić, Milka

Article (Published version)
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Show full item recordAbstract
The method to develop a stable glucose biosensor with successive attachment of cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is presented. The cyclic voltammetry (CV) suggests the diffusion control of the glucose oxidation. The obtained biosensor shows a fast electron transfer of k(0) = 20.4 s(-1), high affinity for glucose with the apparent MichaelisMenten constant K-M(app) = 1.15 mM, a low detection limit of 0.94 mM in a linear range 1.5-7 mM. This biosensor exhibits good stability and reproducibility. Good biocompatibility of modified electrode surface, which enhances the covalent bonded enzyme and consequently glucose oxidation, resulted in biosensor with excellent performances. Biosensor was tested in samples containing human serum.
Keywords:
Biosensors / Immobilization / Enzyme catalysis / Self-assemblySource:
International Journal of Electrochemical Science, 2017, 12, 7, 5806-5817Publisher:
- Esg, Belgrade
Funding / projects:
- Study of the Synthesis, Structure and Activity of Natural and Synthetic Organic Compounds (RS-172013)
- New approach in designing materials for energy conversion and energy storage systems (RS-172060)
DOI: 10.20964/2017.07.65
ISSN: 1452-3981
WoS: 000406090700001
Scopus: 2-s2.0-85023746929
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IHTMTY - JOUR AU - Lović, Jelena AU - Stevanović, Sanja AU - Nikolić, Nebojša D. AU - Petrovic, S. AU - Vukovic, D. AU - Prlainović, Nevena Ž. AU - Mijin, Dušan AU - Avramov Ivić, Milka PY - 2017 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/2141 AB - The method to develop a stable glucose biosensor with successive attachment of cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is presented. The cyclic voltammetry (CV) suggests the diffusion control of the glucose oxidation. The obtained biosensor shows a fast electron transfer of k(0) = 20.4 s(-1), high affinity for glucose with the apparent MichaelisMenten constant K-M(app) = 1.15 mM, a low detection limit of 0.94 mM in a linear range 1.5-7 mM. This biosensor exhibits good stability and reproducibility. Good biocompatibility of modified electrode surface, which enhances the covalent bonded enzyme and consequently glucose oxidation, resulted in biosensor with excellent performances. Biosensor was tested in samples containing human serum. PB - Esg, Belgrade T2 - International Journal of Electrochemical Science T1 - Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode VL - 12 IS - 7 SP - 5806 EP - 5817 DO - 10.20964/2017.07.65 ER -
@article{ author = "Lović, Jelena and Stevanović, Sanja and Nikolić, Nebojša D. and Petrovic, S. and Vukovic, D. and Prlainović, Nevena Ž. and Mijin, Dušan and Avramov Ivić, Milka", year = "2017", abstract = "The method to develop a stable glucose biosensor with successive attachment of cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is presented. The cyclic voltammetry (CV) suggests the diffusion control of the glucose oxidation. The obtained biosensor shows a fast electron transfer of k(0) = 20.4 s(-1), high affinity for glucose with the apparent MichaelisMenten constant K-M(app) = 1.15 mM, a low detection limit of 0.94 mM in a linear range 1.5-7 mM. This biosensor exhibits good stability and reproducibility. Good biocompatibility of modified electrode surface, which enhances the covalent bonded enzyme and consequently glucose oxidation, resulted in biosensor with excellent performances. Biosensor was tested in samples containing human serum.", publisher = "Esg, Belgrade", journal = "International Journal of Electrochemical Science", title = "Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode", volume = "12", number = "7", pages = "5806-5817", doi = "10.20964/2017.07.65" }
Lović, J., Stevanović, S., Nikolić, N. D., Petrovic, S., Vukovic, D., Prlainović, N. Ž., Mijin, D.,& Avramov Ivić, M.. (2017). Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode. in International Journal of Electrochemical Science Esg, Belgrade., 12(7), 5806-5817. https://doi.org/10.20964/2017.07.65
Lović J, Stevanović S, Nikolić ND, Petrovic S, Vukovic D, Prlainović NŽ, Mijin D, Avramov Ivić M. Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode. in International Journal of Electrochemical Science. 2017;12(7):5806-5817. doi:10.20964/2017.07.65 .
Lović, Jelena, Stevanović, Sanja, Nikolić, Nebojša D., Petrovic, S., Vukovic, D., Prlainović, Nevena Ž., Mijin, Dušan, Avramov Ivić, Milka, "Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode" in International Journal of Electrochemical Science, 12, no. 7 (2017):5806-5817, https://doi.org/10.20964/2017.07.65 . .