Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode
Nikolić, Nebojša D.
Prlainović, Nevena Ž.
Avramov Ivić, Milka
Article (Published version)
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The method to develop a stable glucose biosensor with successive attachment of cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is presented. The cyclic voltammetry (CV) suggests the diffusion control of the glucose oxidation. The obtained biosensor shows a fast electron transfer of k(0) = 20.4 s(-1), high affinity for glucose with the apparent MichaelisMenten constant K-M(app) = 1.15 mM, a low detection limit of 0.94 mM in a linear range 1.5-7 mM. This biosensor exhibits good stability and reproducibility. Good biocompatibility of modified electrode surface, which enhances the covalent bonded enzyme and consequently glucose oxidation, resulted in biosensor with excellent performances. Biosensor was tested in samples containing human serum.
Keywords:Biosensors / Immobilization / Enzyme catalysis / Self-assembly
Source:International Journal of Electrochemical Science, 2017, 12, 7, 5806-5817
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