The method to develop a stable glucose biosensor with successive attachment of cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is presented. The cyclic voltammetry (CV) suggests the diffusion control of the glucose oxidation. The obtained biosensor shows a fast electron transfer of k(0) = 20.4 s(-1), high affinity for glucose with the apparent MichaelisMenten constant K-M(app) = 1.15 mM, a low detection limit of 0.94 mM in a linear range 1.5-7 mM. This biosensor exhibits good stability and reproducibility. Good biocompatibility of modified electrode surface, which enhances the covalent bonded enzyme and consequently glucose oxidation, resulted in biosensor with excellent performances. Biosensor was tested in samples containing human serum.
TY - JOUR
AU - Lović, Jelena
AU - Stevanović, Sanja
AU - Nikolić, Nebojša D.
AU - Petrovic, S.
AU - Vukovic, D.
AU - Prlainović, Nevena Ž.
AU - Mijin, Dušan
AU - Avramov Ivić, Milka
PY - 2017
UR - http://cer.ihtm.bg.ac.rs/handle/123456789/2141
AB - The method to develop a stable glucose biosensor with successive attachment of cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is presented. The cyclic voltammetry (CV) suggests the diffusion control of the glucose oxidation. The obtained biosensor shows a fast electron transfer of k(0) = 20.4 s(-1), high affinity for glucose with the apparent MichaelisMenten constant K-M(app) = 1.15 mM, a low detection limit of 0.94 mM in a linear range 1.5-7 mM. This biosensor exhibits good stability and reproducibility. Good biocompatibility of modified electrode surface, which enhances the covalent bonded enzyme and consequently glucose oxidation, resulted in biosensor with excellent performances. Biosensor was tested in samples containing human serum.
PB - Esg, Belgrade
T2 - International Journal of Electrochemical Science
T1 - Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode
VL - 12
IS - 7
SP - 5806
EP - 5817
DO - 10.20964/2017.07.65
ER -
@article{
author = "Lović, Jelena and Stevanović, Sanja and Nikolić, Nebojša D. and Petrovic, S. and Vukovic, D. and Prlainović, Nevena Ž. and Mijin, Dušan and Avramov Ivić, Milka",
year = "2017",
url = "http://cer.ihtm.bg.ac.rs/handle/123456789/2141",
abstract = "The method to develop a stable glucose biosensor with successive attachment of cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is presented. The cyclic voltammetry (CV) suggests the diffusion control of the glucose oxidation. The obtained biosensor shows a fast electron transfer of k(0) = 20.4 s(-1), high affinity for glucose with the apparent MichaelisMenten constant K-M(app) = 1.15 mM, a low detection limit of 0.94 mM in a linear range 1.5-7 mM. This biosensor exhibits good stability and reproducibility. Good biocompatibility of modified electrode surface, which enhances the covalent bonded enzyme and consequently glucose oxidation, resulted in biosensor with excellent performances. Biosensor was tested in samples containing human serum.",
publisher = "Esg, Belgrade",
journal = "International Journal of Electrochemical Science",
title = "Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode",
volume = "12",
number = "7",
pages = "5806-5817",
doi = "10.20964/2017.07.65"
}
Lović, J., Stevanović, S., Nikolić, N. D., Petrovic, S., Vukovic, D., Prlainović, N. Ž., Mijin, D.,& Avramov Ivić, M. (2017). Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode.
International Journal of Electrochemical Science
Esg, Belgrade., 12(7), 5806-5817.
https://doi.org/10.20964/2017.07.65
Lović J, Stevanović S, Nikolić ND, Petrovic S, Vukovic D, Prlainović NŽ, Mijin D, Avramov Ivić M. Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode. International Journal of Electrochemical Science. 2017;12(7):5806-5817
Lović Jelena, Stevanović Sanja, Nikolić Nebojša D., Petrovic S., Vukovic D., Prlainović Nevena Ž., Mijin Dušan, Avramov Ivić Milka, "Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode" International Journal of Electrochemical Science, 12, no. 7 (2017):5806-5817,
https://doi.org/10.20964/2017.07.65 .
