Show simple item record

dc.creatorĐurić, Aleksandra
dc.creatorGojgić-Cvijović, Gordana
dc.creatorJakovljević, Dragica
dc.creatorKekez, Branka
dc.creatorKojic, Jovana Stefanovic
dc.creatorMattinen, Maija-Liisa
dc.creatorHarju, Inka Elina
dc.creatorVrvić, Miroslav
dc.creatorBeškoski, Vladimir
dc.date.accessioned2019-01-30T17:53:24Z
dc.date.available2019-01-30T17:53:24Z
dc.date.issued2017
dc.identifier.issn0141-8130
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/2097
dc.description.abstractVarious microorganisms isolated from polluted environments, such as Pseudomonas sp. and Micrococcus sp. can synthesize exopolysaccharides (EPSs) which are natural, non-toxic and biodegradable polymers. EPSs play a key role in protection of microbial cells under various external influences. For humans, these substances have potential use in many industries. EPSs can be applied as a flavor or a fragrance carrier, an emulsifier, a stabilizer, a prebiotic, an antioxidant or an antitumor agent. In this study, we characterized an environmental microorganism that produces EPS, optimized EPS production by this strain and characterized the EPS produced. Isolate CH-KOV3 was identified as Brachybacterium paraconglomeratum. The sucrose level in the growth medium greatly influenced EPS production, and the highest yield was when the microorganism was incubated in media with 500 g/L, of sucrose. The optimal temperature and pH were 28 degrees C and 7.0, respectively. The nuclear magnetic resonance (NMR) results and GC MS analysis confirmed that the residues were D-fructofuranosyl residues with beta-configuration, where fructose units are linked by beta-2,6-glycosidic bonds, with beta-2,1-linked branches. All these data indicate that the investigated EPS is a levan-type polysaccharide. Thus, it was concluded that Brachybacterium sp. CH-KOV3 could constitute a new source for production of the bioactive polysaccharide, levan.en
dc.publisherElsevier Science Bv, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43004/RS//
dc.relationFCUB ERA
dc.relationJapan International Cooperation Agency (JICA)
dc.rightsrestrictedAccess
dc.sourceInternational Journal of Biological Macromolecules
dc.subjectPolluted environmentsen
dc.subjectExopolysaccharidesen
dc.subjectBrachybacterium paraconglomeratumen
dc.titleBrachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levanen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЈаковљевић, Драгица; Врвиц, Мирослав М.; Маттинен, Маија-Лииса; Кекез, Бранка; Којиц, Јована Стефановиц; Харју, Инка Елина; Бескоски, Владимир П.; Ђурић, Aлександра; Гојгић Цвијовић, Гордана;
dc.citation.volume104
dc.citation.spage311
dc.citation.epage321
dc.citation.other104: 311-321
dc.citation.rankM21
dc.description.otherThe peer-reviewed version: [http://cer.ihtm.bg.ac.rs/handle/123456789/3017]
dc.identifier.pmid28602989
dc.identifier.doi10.1016/j.ijbiomac.2017.06.034
dc.identifier.rcubConv_3815
dc.identifier.scopus2-s2.0-85020640842
dc.identifier.wos000412959300035
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record