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dc.creatorJanović, Barbara
dc.creatorCollins, Andrew R.
dc.creatorVujčić, Zoran
dc.creatorVujčić, Miroslava
dc.date.accessioned2019-01-30T17:52:50Z
dc.date.available2019-01-30T17:52:50Z
dc.date.issued2017
dc.identifier.issn0304-3894
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/2070
dc.description.abstractThe aim of this study was to investigate the impact of dyes on DNA before and after enzymatic decolorization by acidic horseradish peroxidase (HRP-A). The comet assay is easy and feasible method widely used to measure DNA damage and repair. The medium-throughput comet assay was employed for assessment of genotoxic effects of 8 dyes in BEAS-2B cells. We have incorporated a digestion with bacterial endonuclease (formamidopyrimidine DNA glycosylase, FPG) to detect oxidized bases in the case of single and double azo dyes, Orange II (OR2) and Amido Black 10B (AB), respectively. This allowed detection 8-oxo7,8-dihydroguanine, one of most abundant oxidized bases in nuclear DNA. In the case of AB there was no indication of DNA damage, either strand brakes or FPG-sensitive sites before and after decolorization. The OR2 induced DNA damage (in terms of percentage of DNA in comet tails). Also, the frequency of FPG-sensitive sites increased with OR2 concentration. After decolorization no DNA damaging effects was seen at all. The interaction studies of OR2 and AB, before and after decolorization, with calf thymus DNA has been investigated by absorption and fluorescence spectroscopy. The results provide support for the idea that in some cases enzymatic decolorization contributes to lower genotoxicity potential.en
dc.publisherElsevier Science Bv, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172048/RS//
dc.relationFEBS Collaborative Experimental Scholarship, Oslo, Norway
dc.rightsrestrictedAccess
dc.sourceJournal of Hazardous Materials
dc.subjectDNA damageen
dc.subjectComet assayen
dc.subjectHorseradish peroxidase aen
dc.subjectDecolorizationen
dc.subjectDye degradationen
dc.subjectDNA interactionsen
dc.titleAcidic horseradish peroxidase activity abolishes genotoxicity of common dyesen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЈановић, Барбара; Цоллинс, Aндреw Р.; Вујчић, Мирослава; Вујциц, Зоран М.;
dc.citation.volume321
dc.citation.spage576
dc.citation.epage585
dc.citation.other321: 576-585
dc.citation.rankaM21
dc.description.otherThe peer-reviewed version: [http://cer.ihtm.bg.ac.rs/handle/123456789/3045]
dc.identifier.pmid27694021
dc.identifier.doi10.1016/j.jhazmat.2016.09.037
dc.identifier.rcubConv_3634
dc.identifier.scopus2-s2.0-84988963283
dc.identifier.wos000388777300061
dc.type.versionpublishedVersion


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