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dc.creatorLennicke, Claudia
dc.creatorRahn, Jette
dc.creatorKipp, Anna P.
dc.creatorDojčinović, Biljana
dc.creatorMueler, Andreas S.
dc.creatorWessjohann, Ludger A.
dc.creatorLichtenfels, Rudolf
dc.creatorSeliger, Barbara
dc.date.accessioned2019-01-30T17:52:39Z
dc.date.available2019-01-30T17:52:39Z
dc.date.issued2017
dc.identifier.issn0304-4165
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/2061
dc.description.abstractBackground: Selenium (Se) exerts its biological activity largely via selenoproteins, which are key enzymes for maintaining the cellular redox homeostasis. However, besides these beneficial effects there is also evidence that an oversupply of Se might increase the risk towards developing metabolic disorders. To address this in more detail, we directly compared effects of feeding distinct Se compounds and concentrations on hepatic metabolism and expression profiles of mice. Methods: Male C57BL6/J mice received either a selenium-deficient diet or diets enriched with adequate or high doses of selenite, selenate or selenomethionine for 20 weeks. Subsequently, metabolic parameters, enzymatic activities and expression levels of hepatic selenoproteins, Nrf2 targets, and additional redox-sensitive proteins were analyzed. Furthermore, 2D-DIGE-based proteomic profiling revealed Se compound-specific differentially expressed proteins. Results: Whereas heterogeneous effects between high concentrations of the Se compounds were observed with regard to body weight and metabolic activities, selenoproteins were only marginally increased by high Se concentrations in comparison to the respective adequate feeding. In particular the high-SeMet group showed a unique response compromising higher hepatic Se levels in comparison to all other groups. Accordingly, hepatic glutathione (GSH) levels, glutathione S-transferase (GST) activity, and GSTpi1 expression were comparably high in the high-SeMet and Se-deficient group, indicating that compound-specific effects of high doses appear to be independent of selenoproteins. Conclusions: Not only the nature, but also the concentration of Se compounds differentially affect biological processes. General significance: Thus, it is important to consider Se compound-specific effects when supplementing with selenium.en
dc.publisherElsevier Science Bv, Amsterdam
dc.relationDFG - LI1527/3-1
dc.relationDFG - WE1467/13-1
dc.relationDFG - MU3275/3-1
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172030/RS//
dc.rightsrestrictedAccess
dc.sourceBiochimica et Biophysica Acta-General Subjects
dc.subjectSeleniumen
dc.subjectLiveren
dc.subjectRedox statusen
dc.subjectEnergy metabolismen
dc.titleIndividual effects of different selenocompounds on the hepatic proteome and energy metabolism of miceen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractКипп, Aнна П.; Ленницке, Цлаудиа; Селигер, Барбара; Лицхтенфелс, Рудолф; Wессјоханн, Лудгер A.; Муелер, Aндреас С.; Рахн, Јетте; Дојчиновић, Биљана;
dc.citation.volume1861
dc.citation.issue1
dc.citation.spage3323
dc.citation.epage3334
dc.citation.other1861(1): 3323-3334
dc.citation.rankM21
dc.description.otherThe peer-reviewed version: [http://cer.ihtm.bg.ac.rs/handle/123456789/3035]
dc.identifier.pmid27565357
dc.identifier.doi10.1016/j.bbagen.2016.08.015
dc.identifier.rcubConv_3652
dc.identifier.scopus2-s2.0-84995529961
dc.identifier.wos000390736300038
dc.type.versionpublishedVersion


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