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dc.creatorNikolić, Jasna
dc.creatorNešić, Andrijana
dc.creatorČavić, Milena
dc.creatorĐorđević, Neda O.
dc.creatorAnđelković, Uroš
dc.creatorAtanasković-Marković, Marina
dc.creatorDrakulić, Branko
dc.creatorGavrović-Jankulović, Marija
dc.date.accessioned2019-01-30T17:52:36Z
dc.date.available2019-01-30T17:52:36Z
dc.date.issued2017
dc.identifier.issn0304-4165
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/2058
dc.description.abstractBackground: Protein oxidation can occur as a consequence of lipid peroxidation during food processing. The aim of this work was to explore the effect of malondialdehyde (MDA) modification of ovalbumin (OVA) on its interaction with T84 intestinal cells. Methods: Molecular dynamics simulation was employed for the prediction of MDA modification in the OVA, while introduced structural changes were evaluated by measurement of carbonyl group content, fluorescence spectra, MS/MS analysis, and IgE reactivity. Effects of MDA modified OVA on T84 epithelial cells were analyzed by gene expression for pro-inflammatory cytokines and protein secretion. Results: Out of 9 predicted, five modified Lys residues were confirmed by MS/MS analysis: (51)TQINKVVR(58), (85)DILNQITKPNDVYSFSLASR(104), (111)YPILPEYLQCVKELYR(126), (187)AFKDEDTQAMPFR(99), (KIKVYLPR284)-K-277, and (IKVYLPR284)-I-278. The introduced MDA modifications influenced profile of IgE reactivity to OVA. Treatment of T84 epithelial cells with OVA and OVA modified with 1 mM MDA, induced up-regulation of pro-inflammatory cytokines (IL-1 beta,IL-25, IL-33, TSLP and TNF alpha), while OVA modification with 10 mM MDA induced down regulation of the cytokine expression profile, except for IL-1 beta. OVA and OVA modified with 1 mM MDA induced secretion of epithelial cells specific cytokine IL-33. Conclusions: This finding indicated that OVA and its MDA modified form have the potential to trigger the innate immunity by inducing up-regulation and secretion of pro-allergenic IL-33 in T84 intestinal epithelial cells. General significance: Interactions of ovalbumin and its MDA modified form with intestinal epithelial cells can induce a specific immunological priming necessary for the downstream activation of innate immunity.en
dc.publisherElsevier Science Bv, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//
dc.rightsrestrictedAccess
dc.sourceBiochimica et Biophysica Acta-General Subjects
dc.subjectEpithelial cellsen
dc.subjectlnterleukin-33en
dc.subjectMalondialdehydeen
dc.subjectMolecular dynamicsen
dc.subjectOvalbuminen
dc.titleEffect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cellsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractДракулиц, Бранко; Дордевиц, Неда; Гавровиц-Јанкуловиц, Марија; Николиц, Јасна; Aнђелковић, Урош; Aтанасковић-Марковић, Марина; Несиц, Aндријана; Цавиц, Милена;
dc.citation.volume1861
dc.citation.issue2
dc.citation.spage126
dc.citation.epage134
dc.citation.other1861(2): 126-134
dc.citation.rankM21
dc.description.otherThe peer-reviewed version:[http://cer.ihtm.bg.ac.rs/handle/123456789/3042]
dc.identifier.pmid27864150
dc.identifier.doi10.1016/j.bbagen.2016.11.021
dc.identifier.rcubConv_3664
dc.identifier.scopus2-s2.0-84997501265
dc.identifier.wos000392680200013
dc.type.versionpublishedVersion


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