Determination of Microelements in Human Milk and Infant Formula Without Digestion by ICP-OES

2017
Authors
Đurović, DijanaMilisavljevic, Branka
Nedovic-Vukovic, Mirjana
Potkonjak, Branislav
Spasić, Snežana

Vrvić, Miroslav

Article (Published version)
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The concentrations of zinc (Zn), iron (Fe) and copper (Cu) in both human milk and infant formula were determined using a new sample preparation method, by inductively coupled plasma -optical emission spectometry (ICP-OES) and flame atomic absorption spectrometry (FAAS). Human milk samples were diluted in ultrapure water. The infant formula of powder samples (suitable for an infant 1-6 months of age) and standard reference material (SRM-1849) were analyzed in parallel. The results have shown that FAAS method was more sensitive for Fe determination in human milk while ICP-OES was more sensitive for both Zn and Cu detection. The limit of quantification for both Zn and Cu was 5 mu g L-1 and 10 mu g L-1 for Fe and the recovery for Zn, Fe and Cu was ranged from 90% to 94%, 97% to 103% and 90% to 102%, respectively. Mean concentrations of Zn, Fe, and Cu in human milk samples were 5.35, 0.47 and 0.83 mg L-1, respectively while these values in infant formula were ranged from 3.52-4.75 mg L-1, 3....37-4.56 mg L-1 and 0.28-0.41 mg L-1, respectively. Despite the sample complexity, the proposed method using dilution of milk samples with water was simple, rapid, effective and accurate. ICP-OES was a better method for Zn determination while FAAS was a better method for Fe determination. In the case of Cu both methods were comparable.
Keywords:
Microelements / human milk / method validation / sample preparationSource:
Acta Chimica Slovenica, 2017, 64, 2, 276-282Publisher:
- Slovenian Chemical Society
Funding / projects:
DOI: 10.17344/acsi.2016.2582
ISSN: 1318-0207
PubMed: 28621407
WoS: 000405558200003
Scopus: 2-s2.0-85020856021
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IHTMTY - JOUR AU - Đurović, Dijana AU - Milisavljevic, Branka AU - Nedovic-Vukovic, Mirjana AU - Potkonjak, Branislav AU - Spasić, Snežana AU - Vrvić, Miroslav PY - 2017 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/2055 AB - The concentrations of zinc (Zn), iron (Fe) and copper (Cu) in both human milk and infant formula were determined using a new sample preparation method, by inductively coupled plasma -optical emission spectometry (ICP-OES) and flame atomic absorption spectrometry (FAAS). Human milk samples were diluted in ultrapure water. The infant formula of powder samples (suitable for an infant 1-6 months of age) and standard reference material (SRM-1849) were analyzed in parallel. The results have shown that FAAS method was more sensitive for Fe determination in human milk while ICP-OES was more sensitive for both Zn and Cu detection. The limit of quantification for both Zn and Cu was 5 mu g L-1 and 10 mu g L-1 for Fe and the recovery for Zn, Fe and Cu was ranged from 90% to 94%, 97% to 103% and 90% to 102%, respectively. Mean concentrations of Zn, Fe, and Cu in human milk samples were 5.35, 0.47 and 0.83 mg L-1, respectively while these values in infant formula were ranged from 3.52-4.75 mg L-1, 3.37-4.56 mg L-1 and 0.28-0.41 mg L-1, respectively. Despite the sample complexity, the proposed method using dilution of milk samples with water was simple, rapid, effective and accurate. ICP-OES was a better method for Zn determination while FAAS was a better method for Fe determination. In the case of Cu both methods were comparable. PB - Slovenian Chemical Society T2 - Acta Chimica Slovenica T1 - Determination of Microelements in Human Milk and Infant Formula Without Digestion by ICP-OES VL - 64 IS - 2 SP - 276 EP - 282 DO - 10.17344/acsi.2016.2582 ER -
@article{ author = "Đurović, Dijana and Milisavljevic, Branka and Nedovic-Vukovic, Mirjana and Potkonjak, Branislav and Spasić, Snežana and Vrvić, Miroslav", year = "2017", abstract = "The concentrations of zinc (Zn), iron (Fe) and copper (Cu) in both human milk and infant formula were determined using a new sample preparation method, by inductively coupled plasma -optical emission spectometry (ICP-OES) and flame atomic absorption spectrometry (FAAS). Human milk samples were diluted in ultrapure water. The infant formula of powder samples (suitable for an infant 1-6 months of age) and standard reference material (SRM-1849) were analyzed in parallel. The results have shown that FAAS method was more sensitive for Fe determination in human milk while ICP-OES was more sensitive for both Zn and Cu detection. The limit of quantification for both Zn and Cu was 5 mu g L-1 and 10 mu g L-1 for Fe and the recovery for Zn, Fe and Cu was ranged from 90% to 94%, 97% to 103% and 90% to 102%, respectively. Mean concentrations of Zn, Fe, and Cu in human milk samples were 5.35, 0.47 and 0.83 mg L-1, respectively while these values in infant formula were ranged from 3.52-4.75 mg L-1, 3.37-4.56 mg L-1 and 0.28-0.41 mg L-1, respectively. Despite the sample complexity, the proposed method using dilution of milk samples with water was simple, rapid, effective and accurate. ICP-OES was a better method for Zn determination while FAAS was a better method for Fe determination. In the case of Cu both methods were comparable.", publisher = "Slovenian Chemical Society", journal = "Acta Chimica Slovenica", title = "Determination of Microelements in Human Milk and Infant Formula Without Digestion by ICP-OES", volume = "64", number = "2", pages = "276-282", doi = "10.17344/acsi.2016.2582" }
Đurović, D., Milisavljevic, B., Nedovic-Vukovic, M., Potkonjak, B., Spasić, S.,& Vrvić, M.. (2017). Determination of Microelements in Human Milk and Infant Formula Without Digestion by ICP-OES. in Acta Chimica Slovenica Slovenian Chemical Society., 64(2), 276-282. https://doi.org/10.17344/acsi.2016.2582
Đurović D, Milisavljevic B, Nedovic-Vukovic M, Potkonjak B, Spasić S, Vrvić M. Determination of Microelements in Human Milk and Infant Formula Without Digestion by ICP-OES. in Acta Chimica Slovenica. 2017;64(2):276-282. doi:10.17344/acsi.2016.2582 .
Đurović, Dijana, Milisavljevic, Branka, Nedovic-Vukovic, Mirjana, Potkonjak, Branislav, Spasić, Snežana, Vrvić, Miroslav, "Determination of Microelements in Human Milk and Infant Formula Without Digestion by ICP-OES" in Acta Chimica Slovenica, 64, no. 2 (2017):276-282, https://doi.org/10.17344/acsi.2016.2582 . .