Amyloglucosidase from A. niger was covalently immobilized onto poly( GMA-co-EGDMA) by the glutaraldehyde and periodate method. The immobilization of amyloglucosidase after periodate oxidation gave a preparate with the highest specific activity reported so far on similar polymers. The obtained immobilized preparates show the same pH optimum, but a higher temperature optimum compared with the soluble enzyme. The kinetic parameters for the hydrolysis of soluble starch by free and both immobilized enzymes were determined.
Amiloglukozidaza iz A.niger je imobilizovana na poly(GMA-co-EGDMA) glutaraldehidnom i perjodatnom metodom. Imobilizacija amiloglukozidaze nakon perjodatne oksidacije daje preparat sa najvećom do sada objavljenom specifičnom aktivnosti na sličnim polimerima. Dobijeni imobilizovani preparat ima isti pH optimum ali povećani termooptimum u poređenju sa rastvornim enzimom. Određeni su i kinetički parametri za hidrolizu rastvornog skroba imobilizovanim kao i rastvornim enzimom.
TY - JOUR
AU - Milosavic, N
AU - Prodanović, Radivoje
AU - Jovanovic, S.
AU - Novaković, Irena
AU - Vujčić, Zoran
PY - 2005
UR - https://cer.ihtm.bg.ac.rs/handle/123456789/205
AB - Amyloglucosidase from A. niger was covalently immobilized onto poly( GMA-co-EGDMA) by the glutaraldehyde and periodate method. The immobilization of amyloglucosidase after periodate oxidation gave a preparate with the highest specific activity reported so far on similar polymers. The obtained immobilized preparates show the same pH optimum, but a higher temperature optimum compared with the soluble enzyme. The kinetic parameters for the hydrolysis of soluble starch by free and both immobilized enzymes were determined.
AB - Amiloglukozidaza iz A.niger je imobilizovana na poly(GMA-co-EGDMA) glutaraldehidnom i perjodatnom metodom. Imobilizacija amiloglukozidaze nakon perjodatne oksidacije daje preparat sa najvećom do sada objavljenom specifičnom aktivnosti na sličnim polimerima. Dobijeni imobilizovani preparat ima isti pH optimum ali povećani termooptimum u poređenju sa rastvornim enzimom. Određeni su i kinetički parametri za hidrolizu rastvornog skroba imobilizovanim kao i rastvornim enzimom.
PB - Serbian Chemical Soc, Belgrade
T2 - Journal of the Serbian Chemical Society
T1 - Pereparation and characterization of two types of covalently immobilized amyloglucosidase
T1 - Dobijanje i karakterizacija dva tipa imobilizovane amiloglukozidaze
VL - 70
IS - 5
SP - 713
EP - 719
DO - 10.2298/JSC0505713M
ER -
@article{
author = "Milosavic, N and Prodanović, Radivoje and Jovanovic, S. and Novaković, Irena and Vujčić, Zoran",
year = "2005",
abstract = "Amyloglucosidase from A. niger was covalently immobilized onto poly( GMA-co-EGDMA) by the glutaraldehyde and periodate method. The immobilization of amyloglucosidase after periodate oxidation gave a preparate with the highest specific activity reported so far on similar polymers. The obtained immobilized preparates show the same pH optimum, but a higher temperature optimum compared with the soluble enzyme. The kinetic parameters for the hydrolysis of soluble starch by free and both immobilized enzymes were determined., Amiloglukozidaza iz A.niger je imobilizovana na poly(GMA-co-EGDMA) glutaraldehidnom i perjodatnom metodom. Imobilizacija amiloglukozidaze nakon perjodatne oksidacije daje preparat sa najvećom do sada objavljenom specifičnom aktivnosti na sličnim polimerima. Dobijeni imobilizovani preparat ima isti pH optimum ali povećani termooptimum u poređenju sa rastvornim enzimom. Određeni su i kinetički parametri za hidrolizu rastvornog skroba imobilizovanim kao i rastvornim enzimom.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Pereparation and characterization of two types of covalently immobilized amyloglucosidase, Dobijanje i karakterizacija dva tipa imobilizovane amiloglukozidaze",
volume = "70",
number = "5",
pages = "713-719",
doi = "10.2298/JSC0505713M"
}
Milosavic, N., Prodanović, R., Jovanovic, S., Novaković, I.,& Vujčić, Z.. (2005). Pereparation and characterization of two types of covalently immobilized amyloglucosidase. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 70(5), 713-719.
https://doi.org/10.2298/JSC0505713M
Milosavic N, Prodanović R, Jovanovic S, Novaković I, Vujčić Z. Pereparation and characterization of two types of covalently immobilized amyloglucosidase. in Journal of the Serbian Chemical Society. 2005;70(5):713-719.
doi:10.2298/JSC0505713M .
Milosavic, N, Prodanović, Radivoje, Jovanovic, S., Novaković, Irena, Vujčić, Zoran, "Pereparation and characterization of two types of covalently immobilized amyloglucosidase" in Journal of the Serbian Chemical Society, 70, no. 5 (2005):713-719,
https://doi.org/10.2298/JSC0505713M . .
